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Human FGE ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Human FGE in Human in Cell culture supernatant, Plasma, Serum samples.

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Key facts

Detection method

Colorimetric

Sample types

Cell culture supernatant, Plasma, Serum

Assay type

Sandwich (quantitative)

Reactive species

Human

Range

1.45 - 400 ng/mL

Sensitivity

= 1.45 ng/mL

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Reactivity data

Application

sELISA

Reactivity

Reacts

Dilution info

-

Notes

-

Target data

Function

Oxidase that catalyzes the conversion of cysteine to 3-oxoalanine on target proteins, using molecular oxygen and an unidentified reducing agent (PubMed:12757706, PubMed:15657036, PubMed:15907468, PubMed:25931126, PubMed:16368756, PubMed:21224894). 3-oxoalanine modification, which is also named formylglycine (fGly), occurs in the maturation of arylsulfatases and some alkaline phosphatases that use the hydrated form of 3-oxoalanine as a catalytic nucleophile (PubMed:12757706, PubMed:15657036, PubMed:15907468, PubMed:25931126, PubMed:16368756). Known substrates include GALNS, ARSA, STS and ARSE (PubMed:12757706, PubMed:15907468, PubMed:15657036).

Alternative names

What's included?

1 x 96 Tests
Components
150X HRP-Streptavidin
1 x 200 µL
20X Wash Buffer
1 x 25 mL
5X Assay Diluent B (Item E)
1 x 15 mL
Assay Diluent C (Item L)
1 x 30 mL
Biotinylated Human FGE Detection Antibody
2 x 1 Vial
Human FGE Antibody-coated ELISA Plate
1 x 1 Unit
Lyophilized Human FGE Protein Standard
2 x 1 Vial
Stop Solution; 0.2M Sulfuric Acid
1 x 8 mL
TMB One-Step Substrate Reagent
1 x 12 mL

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Human FGE ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Human FGE in Human in Cell culture supernatant, Plasma, Serum samples.

Alternative names

Key facts

Detection method

Colorimetric

Sample types

Cell culture supernatant, Plasma, Serum

Assay type

Sandwich (quantitative)

Reactive species

Human

Range

1.45 - 400 ng/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

Sensitivity

= 1.45 ng/mL

Recovery

Sample specific recovery

Sample type

Serum

Average %

= 123.9

Range

104 - 140 %

Sample type

Plasma

Average %

= 121.4

Range

101 - 139 %

Sample type

Cell culture media

Average %

= 118.6

Range

100 - 136 %

Storage

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

-20°C

Storage information

-20°C

Notes

Human FGE ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human FGE in serum, plasma and cell culture supernatants. This assay employs an antibody specific for human FGE coated on a 96-well plate.

Supplementary info

Biological function summary

Formylglycine-generating enzyme contributes significantly to the functionality of sulfatases which carry out essential steps in the degradation of sulfate-containing molecules. This conversion is not a part of a larger complex but enables critical activities through sulfatases. Sulfatases require formylglycine for activity highlighting FGE's role in regulating their presence and function within the cell.

Activity summary

The FGE or Formylglycine-generating enzyme also known as SUMF1 plays a mechanical role by catalyzing the post-translational activation of sulfatases. This enzyme exists within the endoplasmic reticulum and possesses a molecular weight of approximately 35 kDa. FGE expression occurs broadly across various tissues reflecting its necessity for cellular function. Its action involves converting a specific cysteine residue to formylglycine in sulfatase enzymes essential for their functional activity.

Pathways

Formylglycine-generating enzyme integrates within metabolic pathways involved in glycosaminoglycan degradation. Through this action it connects to proteins like arylsulfatases essential in hydrolyzing sulfate groups from complex carbohydrates. This hydrolysis occurs within the lysosomal degradation pathway where FGE-activated sulfatases function bridging metabolic processes important for cellular maintenance.

Associated diseases and disorders

Mutations or deficiencies in formylglycine-generating enzyme lead to multiple sulfatase deficiency (MSD) a disorder affecting lysosomal function and causing progressive neurological damage. In this context alterations in this enzyme impact related proteins such as ARSA and other sulfatases disrupting normal catabolic processes and leading to the accumulation of sulfated compounds. Additionally FGE deficiencies connect to skeletal dysplasias illustrating the broad physiological impact this enzyme holds when its function is compromised.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

1 product image

  • Sandwich ELISA - Human FGE ELISA Kit (ab314358), expandable thumbnail

    Sandwich ELISA - Human FGE ELISA Kit (ab314358)

    Example of standard curve using ab314358. This standard curve is for demonstrative purposes only. A standard curve must be run with each assay.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

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