JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB314358

Human FGE ELISA Kit

Be the first to review this product! Submit a review

|

(0 Publication)

Human FGE ELISA Kit is a Sandwich ELISA for the measurement of Human FGE in Human in Cell Culture Media, Biofluids samples.

View Alternative Names

PSEC0152, UNQ3037/PRO9852, SUMF1, Formylglycine-generating enzyme, FGE, C-alpha-formylglycine-generating enzyme 1, Sulfatase-modifying factor 1

1 Images
Sandwich ELISA - Human FGE ELISA Kit (AB314358)
  • sELISA

Supplier Data

Sandwich ELISA - Human FGE ELISA Kit (AB314358)

Example of standard curve using ab314358. This standard curve is for demonstrative purposes only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Cell culture supernatant, Plasma, Serum

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 1.45 ng/mL

Range

1.45 - 400 ng/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

style
left-column

Complementary Products

Proteins & Peptides

AB151647

Recombinant Human FGE protein

  • 0
  • 0
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

Product details

Human FGE ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human FGE in serum, plasma and cell culture supernatants. This assay employs an antibody specific for human FGE coated on a 96-well plate.
style
left-column

Recovery

[ { "sample": "Serum", "range": "104 - 140 %", "average": "= 123.9" }, { "sample": "Plasma", "range": "101 - 139 %", "average": "= 121.4" }, { "sample": "Cell culture media", "range": "100 - 136 %", "average": "= 118.6" } ]
style
left-column

What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab314358-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB314358-CMP04", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Human FGE Detection Antibody", "number":"AB314358-CMP10", "productcode":"" }, { "size":"1 x 30 mL", "name":"Assay Diluent C (Item L)", "number":"AB314358-CMP06", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB314358-CMP08", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Human FGE Antibody-coated ELISA Plate", "number":"AB314358-CMP09", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B (Item E)", "number":"AB314358-CMP05", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Lyophilized Human FGE Protein Standard", "number":"AB314358-CMP11", "productcode":"" }, { "size":"1 x 200 µL", "name":"150X HRP-Streptavidin", "number":"AB314358-CMP03", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution; 0.2M Sulfuric Acid", "number":"AB314358-CMP07", "productcode":"" } ] } } }
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The FGE or Formylglycine-generating enzyme also known as SUMF1 plays a mechanical role by catalyzing the post-translational activation of sulfatases. This enzyme exists within the endoplasmic reticulum and possesses a molecular weight of approximately 35 kDa. FGE expression occurs broadly across various tissues reflecting its necessity for cellular function. Its action involves converting a specific cysteine residue to formylglycine in sulfatase enzymes essential for their functional activity.
Biological function summary

Formylglycine-generating enzyme contributes significantly to the functionality of sulfatases which carry out essential steps in the degradation of sulfate-containing molecules. This conversion is not a part of a larger complex but enables critical activities through sulfatases. Sulfatases require formylglycine for activity highlighting FGE's role in regulating their presence and function within the cell.

Pathways

Formylglycine-generating enzyme integrates within metabolic pathways involved in glycosaminoglycan degradation. Through this action it connects to proteins like arylsulfatases essential in hydrolyzing sulfate groups from complex carbohydrates. This hydrolysis occurs within the lysosomal degradation pathway where FGE-activated sulfatases function bridging metabolic processes important for cellular maintenance.

Mutations or deficiencies in formylglycine-generating enzyme lead to multiple sulfatase deficiency (MSD) a disorder affecting lysosomal function and causing progressive neurological damage. In this context alterations in this enzyme impact related proteins such as ARSA and other sulfatases disrupting normal catabolic processes and leading to the accumulation of sulfated compounds. Additionally FGE deficiencies connect to skeletal dysplasias illustrating the broad physiological impact this enzyme holds when its function is compromised.
style
left-column

Product protocols

style
left-column

Target data

Oxidase that catalyzes the conversion of cysteine to 3-oxoalanine on target proteins, using molecular oxygen and an unidentified reducing agent (PubMed : 12757706, PubMed : 15657036, PubMed : 15907468, PubMed : 16368756, PubMed : 21224894, PubMed : 25931126). 3-oxoalanine modification, which is also named formylglycine (fGly), occurs in the maturation of arylsulfatases and some alkaline phosphatases that use the hydrated form of 3-oxoalanine as a catalytic nucleophile (PubMed : 12757706, PubMed : 15657036, PubMed : 15907468, PubMed : 16368756, PubMed : 25931126). Known substrates include GALNS, ARSA, STS and ARSE (PubMed : 12757706, PubMed : 15657036, PubMed : 15907468).
See full target information SUMF1
style
left-column
style
left-column
websiteProtocolBooklet
en
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com