Human FGE ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Human FGE in Human in Cell culture supernatant, Plasma, Serum samples.
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Key facts
- Detection method
- Colorimetric
- Sample types
- Cell culture supernatant, Plasma, Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 1.45 - 400 ng/mL
- Sensitivity
- = 1.45 ng/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Oxidase that catalyzes the conversion of cysteine to 3-oxoalanine on target proteins, using molecular oxygen and an unidentified reducing agent (PubMed:12757706, PubMed:15657036, PubMed:15907468, PubMed:16368756, PubMed:21224894, PubMed:25931126). 3-oxoalanine modification, which is also named formylglycine (fGly), occurs in the maturation of arylsulfatases and some alkaline phosphatases that use the hydrated form of 3-oxoalanine as a catalytic nucleophile (PubMed:12757706, PubMed:15657036, PubMed:15907468, PubMed:16368756, PubMed:25931126). Known substrates include GALNS, ARSA, STS and ARSE (PubMed:12757706, PubMed:15657036, PubMed:15907468).
Alternative names
PSEC0152, UNQ3037/PRO9852, SUMF1, Formylglycine-generating enzyme, FGE, C-alpha-formylglycine-generating enzyme 1, Sulfatase-modifying factor 1
What's included?
Recommended products
Human FGE ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Human FGE in Human in Cell culture supernatant, Plasma, Serum samples.
Key facts
- Detection method
- Colorimetric
- Sample types
- Cell culture supernatant, Plasma, Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 1.45 - 400 ng/mL
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 1.45 ng/mL
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Serum | Average % = 123.9 | Range 104 - 140 % |
Sample type Plasma | Average % = 121.4 | Range 101 - 139 % |
Sample type Cell culture media | Average % = 118.6 | Range 100 - 136 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- -20°C
Notes
Human FGE ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human FGE in serum, plasma and cell culture supernatants. This assay employs an antibody specific for human FGE coated on a 96-well plate.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The FGE or Formylglycine-generating enzyme also known as SUMF1 plays a mechanical role by catalyzing the post-translational activation of sulfatases. This enzyme exists within the endoplasmic reticulum and possesses a molecular weight of approximately 35 kDa. FGE expression occurs broadly across various tissues reflecting its necessity for cellular function. Its action involves converting a specific cysteine residue to formylglycine in sulfatase enzymes essential for their functional activity.
- Biological function summary
Formylglycine-generating enzyme contributes significantly to the functionality of sulfatases which carry out essential steps in the degradation of sulfate-containing molecules. This conversion is not a part of a larger complex but enables critical activities through sulfatases. Sulfatases require formylglycine for activity highlighting FGE's role in regulating their presence and function within the cell.
- Pathways
Formylglycine-generating enzyme integrates within metabolic pathways involved in glycosaminoglycan degradation. Through this action it connects to proteins like arylsulfatases essential in hydrolyzing sulfate groups from complex carbohydrates. This hydrolysis occurs within the lysosomal degradation pathway where FGE-activated sulfatases function bridging metabolic processes important for cellular maintenance.
- Associated diseases and disorders
Mutations or deficiencies in formylglycine-generating enzyme lead to multiple sulfatase deficiency (MSD) a disorder affecting lysosomal function and causing progressive neurological damage. In this context alterations in this enzyme impact related proteins such as ARSA and other sulfatases disrupting normal catabolic processes and leading to the accumulation of sulfated compounds. Additionally FGE deficiencies connect to skeletal dysplasias illustrating the broad physiological impact this enzyme holds when its function is compromised.
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1 product image
Sandwich ELISA - Human FGE ELISA Kit (ab314358)
Example of standard curve using ab314358. This standard curve is for demonstrative purposes only. A standard curve must be run with each assay.
Downloads
Product protocols
- Visit the general protocols
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