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AB316908

Human FGF-7/KGF ELISA Kit

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Human FGF-7/KGF ELISA Kit is a single-wash 90-min Simplestep used to quantify Human FGF-7/KGF with a sensitivity of 1.892 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader

View Alternative Names

KGF, FGF7, Fibroblast growth factor 7, FGF-7, Heparin-binding growth factor 7, Keratinocyte growth factor, HBGF-7

4 Images
Sandwich ELISA - Human FGF-7/KGF ELISA Kit (AB316908)
  • sELISA

Supplier Data

Sandwich ELISA - Human FGF-7/KGF ELISA Kit (AB316908)

Interpolated concentration of native FGF-7/KGF was measured in duplicate at different sample concentrations. Undiluted samples are 50% U-87 MG cell culture supernatant. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

Sandwich ELISA - Human FGF-7/KGF ELISA Kit (AB316908)
  • sELISA

Supplier Data

Sandwich ELISA - Human FGF-7/KGF ELISA Kit (AB316908)

Standard curve comparison between Human FGF-7/KGF SimpleStep ELISA kit (ab316908) and traditional ELISA kit from leading competitor. The SimpleStep ELISA kit shows increased sensitivity.

Sandwich ELISA - Human FGF-7/KGF ELISA Kit (AB316908)
  • sELISA

Supplier Data

Sandwich ELISA - Human FGF-7/KGF ELISA Kit (AB316908)

Standard curve comparison between the original Human FGF-7/KGF SimpleStep ELISA (ab183362) and current Human FGF-7/KGF SimpleStep ELISA (ab316908).

Sandwich ELISA - Human FGF-7/KGF ELISA Kit (AB316908)
  • sELISA

Supplier Data

Sandwich ELISA - Human FGF-7/KGF ELISA Kit (AB316908)

Example of human FGF-7/KGF standard curve. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Serum, EDTA Plasma, Citrate plasma, Cell culture media

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 1.892 pg/mL

Range

6.25 - 400 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human FGF-7/KGF SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of FGF-7/KGF protein in Serum, EDTA Plasma, Cit Plasma, and Cell culture media. Quantitate Human FGF-7/KGF with 1.892 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.6" }, { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "1.4" } ]
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Recovery

[ { "sample": "Serum", "range": "92 - 103 %", "average": "= 99" }, { "sample": "EDTA Plasma", "range": "91 - 104 %", "average": "= 99" }, { "sample": "Citrate plasma", "range": "92 - 107 %", "average": "= 100" }, { "sample": "Cell culture media", "range": "97 - 116 %", "average": "= 106" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab316908-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB316908-CMP08", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB316908-CMP06", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB316908-CMP07", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Lyophilized Human FGF-7/KGF Capture Antibody", "number":"AB316908-CMP01", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB316908-CMP05", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BI", "number":"AB316908-CMP04", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human FGF-7/KGF Lyophilized Recombinant Protein", "number":"AB316908-CMP03", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human FGF-7/KGF Detector Antibody", "number":"AB316908-CMP02", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB316908-CMP09", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB316908-CMP10", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

Plays an important role in the regulation of embryonic development, cell proliferation and cell differentiation. Required for normal branching morphogenesis. Growth factor active on keratinocytes. Possible major paracrine effector of normal epithelial cell proliferation.
See full target information FGF7
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websiteProtocolBooklet
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com