Human FGF basic ELISA Kit (FGF2) is a Sandwich (quantitative) ELISA kit for the measurement of Human FGF basic (FGF2) in Human in Plasma, Cell culture supernatant, Serum, Cell Lysate samples.
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- In vivo (Athens, Greece) 37:2057-20692023Effects of the Foam Massage Roller on VEGF-A and FGF-2 Blood Levels in Young Men.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAdam Roslanowski,Aleksandra Partynska,Katarzyna Ratajczak-Wielgomas,Alicja Kmiecik,Jedrzej Grzegrzolka,Piotr Dziegiel,Adam Januszko,Dariusz Lenart,Waldemar AndrzejewskiPubMed 37652524
- Stem cell research & therapy 11:5352020Hypoxia-preconditioned adipose-derived stem cells combined with scaffold promote urethral reconstruction by upregulation of angiogenesis and glycolysis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiang Wan et. al.PubMed 33308306
- PloS one 15:e02426262020TGF-β-induced activation of conjunctival fibroblasts is modulated by FGF-2 and substratum stiffness.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTomoyo Matsumura et. al.PubMed 33206726
- International journal of molecular medicine 46:311-3192020Cyclopamine functions as a suppressor of benign prostatic hyperplasia by inhibiting epithelial and stromal cell proliferation via suppression of the Hedgehog signaling pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYi-Feng Yuan,Wen-Xiong Zhu,Tao Liu,Ju-Qiao He,Qing Zhou,Xing Zhou,Xi Zhang,Jing YangPubMed 32319534
- Journal of cellular and molecular medicine 24:3931-39472020Inhibition of PLK4 might enhance the anti-tumour effect of bortezomib on glioblastoma via PTEN/PI3K/AKT/mTOR signalling pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJing Wang,Dengpeng Ren,Yan Sun,Chao Xu,Chunhong Wang,Rui Cheng,Lina Wang,Guijun Jia,Jinrui Ren,Jiuhong Ma,Yue Tu,Hongming JiPubMed 32126150
- Cytotherapy 22:106-1132020Rhynchophylline promotes stem cell autonomous metabolic homeostasis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYuji Kaneko et. al.PubMed 31983606
- IET nanobiotechnology 14:73-772020Efficient co-cultivation of human fibroblast cells (HFCs) and adipose-derived stem cells (ADSs) on gelatin/PLCL nanofiber.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMarziyeh Ranjbar-Mohammadi,Elham Mousavi,Mohammad Mostakhdem Hashemi,Mahdi Abbasian,Jahanbakhsh Asadi,Ehsan Esmaili,Mehrafarin Fesharaki,Pouyan Asadi,Zahra Arab-BafraniPubMed 31935681
- Cancer science 109:3816-38252018Fibroblast growth factor receptor 3-mediated reactivation of ERK signaling promotes head and neck squamous cancer cell insensitivity to MEK inhibition.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMyung Jin Ban et. al.PubMed 30343534
- Journal of cellular physiology 234:3425-34352018Decellularized breast matrix as bioactive microenvironment for in vitro three-dimensional cancer culture.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQin Jin,Gang Liu,Shubin Li,Haihan Yuan,Zhizhong Yun,Wenqi Zhang,Shu Zhang,Yanfeng Dai,Yuzhen MaPubMed 30387128
- Biomedicine & pharmacotherapy = Biomedecine & phar 107:359-3672018MiR-148b-3p inhibits renal carcinoma cell growth and pro-angiogenic phenotype of endothelial cell potentially by modulating FGF2.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHui Zhang et. al.PubMed 30099339
Key facts
- Detection method
- Colorimetric
- Sample types
- Plasma, Cell culture supernatant, Serum, Cell Lysate
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 102.4 - 10000 pg/mL
- Sensitivity
- < 50 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Acts as a ligand for FGFR1, FGFR2, FGFR3 and FGFR4 (PubMed:8663044). Also acts as an integrin ligand which is required for FGF2 signaling (PubMed:28302677). Binds to integrin ITGAV:ITGB3 (PubMed:28302677). Plays an important role in the regulation of cell survival, cell division, cell differentiation and cell migration (PubMed:28302677, PubMed:8663044). Functions as a potent mitogen in vitro (PubMed:1721615, PubMed:3732516, PubMed:3964259). Can induce angiogenesis (PubMed:23469107, PubMed:28302677). Mediates phosphorylation of ERK1/2 and thereby promotes retinal lens fiber differentiation (PubMed:29501879).
Alternative names
FGFB, FGF2, Fibroblast growth factor 2, FGF-2, Basic fibroblast growth factor, Heparin-binding growth factor 2, bFGF, HBGF-2
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Recommended products
Human FGF basic ELISA Kit (FGF2) is a Sandwich (quantitative) ELISA kit for the measurement of Human FGF basic (FGF2) in Human in Plasma, Cell culture supernatant, Serum, Cell Lysate samples.
Key facts
- Detection method
- Colorimetric
- Sample types
- Plasma, Cell culture supernatant, Serum, Cell Lysate
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 102.4 - 10000 pg/mL
- Assay Platform
- Microplate
- Sensitivity
- < 50 pg/mL
- General recovery
- 94%
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Cell culture supernatant | Average % = 93.48 | Range 83 - 103 % |
Sample type Serum | Average % = 95.46 | Range 84 - 104 % |
Sample type Plasma | Average % = 92.64 | Range 82 - 103 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- -20°C
Notes
Abcam's FGF basic (FGF2) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human FGF basic in serum, plasma, and cell culture supernatants.
This assay employs an antibody specific for Human FGF basic coated on a 96-well plate. Standards and samples are pipetted into the wells and FGF basic present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human FGF basic antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of FGF basic bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Optimization may be required with urine samples.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
FGF basic also known as FGF-2 or bFGF is a member of the fibroblast growth factor family. This protein is approximately 18 kDa in mass and is expressed in various tissues including the brain skin and vascular endothelium. Its alternate forms include FGF-2 and FGF-basic which serve similar functional roles. FGF basic acts mechanically by binding to fibroblast growth factor receptors (FGFRs) initiating a cascade of intracellular signaling that leads to cell growth and differentiation.
- Biological function summary
Fibroblast growth factor 2 is important in development and wound healing. It does not form part of a larger complex but interacts directly with receptors on the cell surface to activate downstream signaling pathways. FGF basic plays an important role in angiogenesis facilitating the growth of new blood vessels. Additionally this protein supports the proliferation of a wide range of cell types including fibroblasts and endothelial cells.
- Pathways
Fibroblast growth factor 2 participates mainly in the MAPK/ERK and PI3K/AKT signaling pathways. These pathways are essential for controlling cell division and survival. In the MAPK/ERK pathway FGF basic's interaction with FGFR involves proteins such as Ras and Raf. Similarly in the PI3K/AKT pathway it engages with proteins including PI3K and Akt coordinating various cellular responses to environmental stimuli.
- Associated diseases and disorders
FGF basic links to various cancers and cardiovascular diseases. In cancer abnormal FGF basic signaling can lead to uncontrolled cell growth where it interacts with oncogenic proteins like Ras. In cardiovascular disorders altered FGF basic expression affects vascular integrity often in conjunction with other proteins like VEGF contributing to pathological conditions such as atherosclerosis.
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4 product images
Sandwich ELISA - Human FGF basic ELISA Kit (FGF2) (ab99979)
Sandwich ELISA - Human FGF basic ELISA Kit (FGF2) (ab99979)
Sandwich ELISA - Human FGF basic ELISA Kit (FGF2) (ab99979)
Representative Standard Curve using ab99979.
Sandwich ELISA - Human FGF basic ELISA Kit (FGF2) (ab99979)
Representative Standard Curve using ab99979.
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