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AB99980

Human FGF basic ELISA Kit (FGF2)

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(1 Publication)

Human FGF basic ELISA Kit (FGF2) is a Sandwich ELISA for the measurement of Human FGF basic (FGF2) in Human in Cell/Tissue Extracts samples.

View Alternative Names

FGFB, FGF2, Fibroblast growth factor 2, FGF-2, Basic fibroblast growth factor, Heparin-binding growth factor 2, bFGF, HBGF-2

3 Images
Sandwich ELISA - Human FGF basic ELISA Kit (FGF2) (AB99980)
  • sELISA

Lab

Sandwich ELISA - Human FGF basic ELISA Kit (FGF2) (AB99980)

Standard curve : mean of duplicates (+/- SD) with background reads subtracted

Sandwich ELISA - Human FGF basic ELISA Kit (FGF2) (AB99980)
  • sELISA

Lab

Sandwich ELISA - Human FGF basic ELISA Kit (FGF2) (AB99980)

FGF2 measured in cell culture lysates showing quantity (pg) per 1 mln cells. Samples with the concentration of 1e7 cells/mL were used. Samples were diluted 200-2000 fold.

Sandwich ELISA - Human FGF basic ELISA Kit (FGF2) (AB99980)
  • sELISA

Lab

Sandwich ELISA - Human FGF basic ELISA Kit (FGF2) (AB99980)

FGF2 measured in tissue lysates showing quantity (pg) per 1 mg of extracted protein. Protein concentration for samples varied from 7 mg/mL to 17 mg/mL. Samples were diluted 200-2000 fold.

Key facts

Detection method

Colorimetric

Sample types

Cell culture extracts, Tissue Extracts

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

< 50 pg/mL

Range

102.4 - 10000 pg/mL

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's FGF basic (FGF2) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human FGF basic in cell lysates and tissue lysates.

This assay employs an antibody specific for Human FGF basic coated on a 96-well plate. Standards and samples are pipetted into the wells and FGF basic present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human FGF basic antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of FGF basic bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Recovery

[ { "sample": "Cell culture extracts", "range": "87 - 108 %", "average": "= 97.65" }, { "sample": "Tissue Extracts", "range": "86 - 107 %", "average": "= 95.87" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab99980-1x96Tests", "assetComponentDetails": [ { "size":"1 x 15 mL", "name":"5X Assay Diluent", "number":"AB99980-CMP04", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB99980-CMP03", "productcode":"" }, { "size":"1 x 5 mL", "name":"2X Cell Lysis Buffer", "number":"AB99980-CMP09", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB99980-CMP07", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB99980-CMP08", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Sample Diluent Buffer", "number":"AB99980-CMP02", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated anti-Human FGF basic", "number":"AB99980-CMP10", "productcode":"" }, { "size":"1 x 200 µL", "name":"120X HRP-Streptavidin Concentrate", "number":"AB99980-CMP01", "productcode":"" }, { "size":"1 x 1 Unit", "name":"FGF basic Microplate (12 x 8 wells)", "number":"AB99980-CMP06", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Recombinant Human FGF basic Standard (lyophilized)", "number":"AB99980-CMP05", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

FGF basic also known as FGF-2 or bFGF is a member of the fibroblast growth factor family. This protein is approximately 18 kDa in mass and is expressed in various tissues including the brain skin and vascular endothelium. Its alternate forms include FGF-2 and FGF-basic which serve similar functional roles. FGF basic acts mechanically by binding to fibroblast growth factor receptors (FGFRs) initiating a cascade of intracellular signaling that leads to cell growth and differentiation.
Biological function summary

Fibroblast growth factor 2 is important in development and wound healing. It does not form part of a larger complex but interacts directly with receptors on the cell surface to activate downstream signaling pathways. FGF basic plays an important role in angiogenesis facilitating the growth of new blood vessels. Additionally this protein supports the proliferation of a wide range of cell types including fibroblasts and endothelial cells.

Pathways

Fibroblast growth factor 2 participates mainly in the MAPK/ERK and PI3K/AKT signaling pathways. These pathways are essential for controlling cell division and survival. In the MAPK/ERK pathway FGF basic's interaction with FGFR involves proteins such as Ras and Raf. Similarly in the PI3K/AKT pathway it engages with proteins including PI3K and Akt coordinating various cellular responses to environmental stimuli.

FGF basic links to various cancers and cardiovascular diseases. In cancer abnormal FGF basic signaling can lead to uncontrolled cell growth where it interacts with oncogenic proteins like Ras. In cardiovascular disorders altered FGF basic expression affects vascular integrity often in conjunction with other proteins like VEGF contributing to pathological conditions such as atherosclerosis.
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Product protocols

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Target data

Acts as a ligand for FGFR1, FGFR2, FGFR3 and FGFR4 (PubMed : 8663044). Also acts as an integrin ligand which is required for FGF2 signaling (PubMed : 28302677). Binds to integrin ITGAV : ITGB3 (PubMed : 28302677). Plays an important role in the regulation of cell survival, cell division, cell differentiation and cell migration (PubMed : 28302677, PubMed : 8663044). Functions as a potent mitogen in vitro (PubMed : 1721615, PubMed : 3732516, PubMed : 3964259). Can induce angiogenesis (PubMed : 23469107, PubMed : 28302677). Mediates phosphorylation of ERK1/2 and thereby promotes retinal lens fiber differentiation (PubMed : 29501879).
See full target information FGF2
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Proceedings of the National Academy of Sciences of the United States of America 111:781-6 PubMed24379381

2014

Potentiation of cytotoxic chemotherapy by growth hormone-releasing hormone agonists.

Applications

Unspecified application

Species

Unspecified reactive species

Miklos Jaszberenyi,Ferenc G Rick,Petra Popovics,Norman L Block,Marta Zarandi,Ren-Zhi Cai,Irving Vidaurre,Luca Szalontay,Arumugam R Jayakumar,Andrew V Schally
View all publications
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websiteProtocolBooklet
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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