AB219636

Human FGF1 ELISA Kit

Recombinant
SimpleStep
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(6 Publications)

Human FGF1 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human FGF1 with a sensitivity of 1.97 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
- Cited in over 5 citations

View Alternative Names

FGFA, FGF1, Fibroblast growth factor 1, FGF-1, Acidic fibroblast growth factor, Endothelial cell growth factor, Heparin-binding growth factor 1, aFGF, ECGF, HBGF-1

7 Images

Sandwich ELISA - Human FGF1 ELISA Kit (AB219636)

sELISA

Supplier Data

Sandwich ELISA - Human FGF1 ELISA Kit (AB219636)

Interpolated concentrations of native FGF1 in human PBMC unstimulated and stimulated media samples.

The concentrations of FGF1 were measured in duplicates and interpolated from the FGF1 standard curves. Undiluted samples are as follows : unstimulated PBMC media 100% and stimulated PBMC media 100%. The interpolated values are plotted (mean +/- SD, n=2). The level of FGF1 in neat unstimulated PBMC media was below the 7^th point of the standard curve and could not be interpolated. The mean FGF1 concentration was determined to be 27 pg/mL in neat stimulated PBMC media. PBMC media samples were cultured in RPMI media with 10% fetal bovine serum (unstimulated) for 24 hours and then stimulated for 48 hours with 1.5% PHAM.

sELISA

Supplier Data

Sandwich ELISA - Human FGF1 ELISA Kit (AB219636)

Example of human FGF1 standard curve in Sample Diluent 50BS.

Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Human FGF1 ELISA Kit (AB219636)

Interpolated concentrations of native FGF1 in human, mouse, and rat brain samples based on 20 μg/mL extract loads for each sample type.

The concentrations of FGF1 were measured in duplicate and interpolated from the FGF1 standard curve. The interpolated values are plotted (mean +/- SD, n=2). The mean FGF1 concentration was determined to be 445 pg/mL in human brain extract, 161 pg/mL in mouse brain extract, and 61 pg/mL in rat brain extract.

sELISA

Supplier Data

Sandwich ELISA - Human FGF1 ELISA Kit (AB219636)

Interpolated concentrations of native FGF1 in human, mouse, and rat brain samples based on 40 μg/mL, 20 μg/mL, and 20 μg/mL extract loads, respectively.

The concentrations of FGF1 were measured in duplicate and interpolated from the FGF1 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean FGF1 concentration was determined to be 886 pg/mL in human brain extract, 179 pg/mL in mouse brain extract, and 68 pg/mL in rat brain extract.

sELISA

Supplier Data

Sandwich ELISA - Human FGF1 ELISA Kit (AB219636)

Example of human FGF1 standard curve in Sample Diluent 1X Cell Extraction buffer PTR.

Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Human FGF1 ELISA Kit (AB219636)

Example of human FGF1 standard curve in Sample Diluent NS.

Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Human FGF1 ELISA Kit (AB219636)

Interpolated concentrations of spiked FGF1 in human serum, urine, saliva, and stimulated PBMC media samples.

The concentrations of FGF1 were measured in duplicates, interpolated from the FGF1 standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 50%, urine 50%, saliva 50%, and stimulated PBMC media 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean FGF1 concentration was determined to be 868 pg/mL in neat serum, 1,275 pg/mL in neat urine, 903 pg/mL in neat saliva, and 518 pg/mL in neat stimulated PBMC culture media.

Key facts

Detection method

Colorimetric

Sample types

Saliva, Urine, Tissue Extracts, Cell culture media, Cell culture supernatant, Serum

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 1.97 pg/mL

Range

18.75 - 1200 pg/mL

Assay time

1h 30m

Assay Platform

Microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Human FGF1 ELISA Kit (ab219636) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of FGF1 protein in cell culture supernatant, saliva, serum, tissue extracts, urine, and cell culture media. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human FGF1 with 1.97 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

Fibroblast growth factor 1 (FGF1) is a heparin-binding fibroblast growth factor that plays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. Specifically, FGF1 binds to FGFR1 in the presence of heparin leading to FGFR1 dimerization and activation. Endogenous FGF-1 is found in the nucleus of most cell types. Nuclear localization is required for FGF-1 mitogenic activity. FGF-1 promotes tumor development by promoting cancer cell proliferation and survival. Mouse and rat FGF1 are 95.5% identical to human FGF1.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Extract", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.8" }, { "reproducibilityType": "Intra", "sample": "Extract", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.4" } ]

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Recovery

[ { "sample": "Serum", "range": "89 - 94 %", "average": "= 91" }, { "sample": "Saliva", "range": "87 - 91 %", "average": "= 88" }, { "sample": "Urine", "range": "78 - 83 %", "average": "= 81" }, { "sample": "Tissue Extracts", "range": "101 - 104 %", "average": "= 103" }, { "sample": "Cell culture media", "range": "112 - 115 %", "average": "= 114" } ]

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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab219636-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB219636-CMP06", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB219636-CMP13", "productcode":"" }, { "size":"1 x 1 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB219636-CMP08", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB219636-CMP04", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB219636-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB219636-CMP12", "productcode":"" }, { "size":"1 x 20 mL", "name":"Sample Diluent 50BS", "number":"AB219636-CMP03", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BI", "number":"AB219636-CMP10", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human FGF1 Lyophilized Recombinant Protein", "number":"AB219636-CMP11", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human FGF1 Capture Antibody", "number":"AB219636-CMP01", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human FGF1 Detector Antibody", "number":"AB219636-CMP02", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB219636-CMP09", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB219636-CMP05", "productcode":"" } ] } } }

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

FGF1 also known as acidic fibroblast growth factor (aFGF) is a member of the fibroblast growth factor family. This protein has a molecular mass of approximately 17 kDa. FGF1 is expressed in a variety of tissues such as the brain liver and heart and plays an essential role in cell growth development and repair. The FGF1 protein is sometimes studied using techniques such as FGF1 ELISA which helps in quantifying and understanding its expression levels and activity in different contexts.

Biological function summary

FGF1 is involved in numerous cellular processes beyond simple cell proliferation. It binds to fibroblast growth factor receptors (FGFRs) on the cell surface initiating a cascade of downstream signaling events. This protein does not act alone; it is often a part of larger protein complexes that modulate cellular processes such as angiogenesis and wound healing. FGF1 is also implicated in nerve cell differentiation and survival through FGFR-mediated pathways.

Pathways

The FGF1 protein is integrally involved in the MAPK/ERK signaling pathway a critical pathway that regulates cell division growth and differentiation. Through this pathway FGF1 interacts with various proteins including Ras Raf and ERK to propagate the signal necessary for mitogenic and survival responses. FGF1 is also a player in the phosphoinositide 3-kinase (PI3K) pathway which helps control cellular responses to stress and metabolic regulation showing its role in cellular homeostasis.

FGF1 is associated with conditions such as cancer and cardiovascular diseases. The deregulation of FGF1 expression or its signaling pathways can lead to tumor development and progression with FGF1 often interacting with other proteins like the basic fibroblast growth factor (bFGF) in these contexts. In cardiovascular diseases altered FGF1 signaling might contribute to atherosclerosis and cardiac repair processes where its interaction with other growth factors like vascular endothelial growth factor (VEGF) is significant.

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Product protocols

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Target data

Plays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. Functions as a potent mitogen in vitro. Acts as a ligand for FGFR1 and integrins. Binds to FGFR1 in the presence of heparin leading to FGFR1 dimerization and activation via sequential autophosphorylation on tyrosine residues which act as docking sites for interacting proteins, leading to the activation of several signaling cascades. Binds to integrin ITGAV : ITGB3. Its binding to integrin, subsequent ternary complex formation with integrin and FGFR1, and the recruitment of PTPN11 to the complex are essential for FGF1 signaling. Induces the phosphorylation and activation of FGFR1, FRS2, MAPK3/ERK1, MAPK1/ERK2 and AKT1 (PubMed : 18441324, PubMed : 20422052). Can induce angiogenesis (PubMed : 23469107).

See full target information FGF1

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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Journal of human reproductive sciences 17:121-127 PubMed39091443

2024

Association of Fibroblast Growth Factor-1 Promoter Polymorphism and its Serum Concentrations with Repeated Implantation Failure after Fertilisation: A Cross-sectional Study.

Applications

Unspecified application

Species

Unspecified reactive species

Afshin Kharamani,Farhad Mashayekhi,Zivar Salehi

Cells 12: PubMed38067126

2023

An Exosome-Rich Conditioned Medium from Human Amniotic Membrane Stem Cells Facilitates Wound Healing via Increased Reepithelization, Collagen Synthesis, and Angiogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Chan Ho Noh,Sangryong Park,Hye-Rim Seong,Ah-Young Lee,Khan-Erdene Tsolmon,Dongho Geum,Soon-Cheol Hong,Tae Myoung Kim,Ehn-Kyoung Choi,Yun-Bae Kim

International journal of molecular sciences 24: PubMed37175778

2023

Intraocular Pressure-Lowering and Retina-Protective Effects of Exosome-Rich Conditioned Media from Human Amniotic Membrane Stem Cells in a Rat Model of Glaucoma.

Applications

Unspecified application

Species

Unspecified reactive species

Hye-Rim Seong,Chan Ho Noh,Sangryong Park,Sumin Cho,Seok-Jin Hong,Ah-Young Lee,Dongho Geum,Soon-Cheol Hong,Dongsun Park,Tae Myoung Kim,Ehn-Kyoung Choi,Yun-Bae Kim

Redox biology 38:101780 PubMed33171331

2020

Regulation of PD-L1 expression in K-ras-driven cancers through ROS-mediated FGFR1 signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Christophe Glorieux,Xiaojun Xia,Yong-Qiao He,Yumin Hu,Kelly Cremer,Annie Robert,Junchen Liu,Fen Wang,Jianhua Ling,Paul J Chiao,Peng Huang

Bioscience reports 39: PubMed31138757

2019

Low concentration of rutin treatment might alleviate the cardiotoxicity effect of pirarubicin on cardiomyocytes via activation of PI3K/AKT/mTOR signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Junjie Fei,Yi Sun,Yuyin Duan,Jianming Xia,Songhua Yu,Peigang Ouyang,Teng Wang,Guimin Zhang

Experimental and therapeutic medicine 18:1005-1012 PubMed31316598

2019

miR-628 reduces prostate cancer proliferation and invasion via the FGFR2 signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Jun Chen,Peng Hao,Tao Zheng,Yong Zhang

View all publications

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websiteProtocolBooklet

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Human FGF1 ELISA Kit

Sandwich ELISA - Human FGF1 ELISA Kit (AB219636)

Sandwich ELISA - Human FGF1 ELISA Kit (AB219636)

Sandwich ELISA - Human FGF1 ELISA Kit (AB219636)

Sandwich ELISA - Human FGF1 ELISA Kit (AB219636)

Sandwich ELISA - Human FGF1 ELISA Kit (AB219636)

Sandwich ELISA - Human FGF1 ELISA Kit (AB219636)

Sandwich ELISA - Human FGF1 ELISA Kit (AB219636)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay time

Assay Platform

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (6)

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Primary Antibodies

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Product promise