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AB327288

Human FGF1 ELISA Kit, Chemiluminescent

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Human FGF1 ELISA Kit, Chemiluminescent is a 55-min single-wash SimpleStep Ignite™ ELISA for the rapid quantification of Human FGF1 with higher sensitivity and broader measuring range.

- Single 30 min incubation and single-wash step for results in less than an hour.
- Chemiluminescent detection allows a broad dynamic range: 3.43 - 2500 pg/ml with a sensitivity of 1.63 pg/mL.
- Different formats for different needs: 10x 96-well plates for bulk orders
- Design your own immunoassay: we also offer the conjugation-ready antibody pair.
- Patent pending
6 Images
Sandwich ELISA - Human FGF1 ELISA Kit, Chemiluminescent (AB327288)
  • sELISA

Supplier Data

Sandwich ELISA - Human FGF1 ELISA Kit, Chemiluminescent (AB327288)

Interpolated concentrations of native FGF1 in human mouse and rat brain samples based on 20 µg/mL extract loads for each sample type.

The concentrations of FGF1 were measured in duplicate and interpolated from the FGF1 standard curve. The interpolated values are plotted (mean +/- SD n=2). The mean FGF1 concentration was determined to be 445 pg/mL in human brain extract 161 pg/mL in mouse brain extract and 61 pg/mL in rat brain extract.

Sandwich ELISA - Human FGF1 ELISA Kit, Chemiluminescent (AB327288)
  • sELISA

Supplier Data

Sandwich ELISA - Human FGF1 ELISA Kit, Chemiluminescent (AB327288)

Interpolated concentrations of native FGF1 in human PBMC unstimulated and stimulated media samples.

The concentrations of FGF1 were measured in duplicates and interpolated from the FGF1 standard curves. Undiluted samples are as follows : unstimulated PBMC media 100% and stimulated PBMC media 100%. The interpolated values are plotted (mean +/- SD n=2). The level of FGF1 in neat unstimulated PBMC media was below the 7th point of the standard curve and could not be interpolated. The mean FGF1 concentration was determined to be 27 pg/mL in neat stimulated PBMC media. PBMC media samples were cultured in RPMI media with 10% fetal bovine serum (unstimulated) for 24 hours and then stimulated for 48 hours with 1.5% PHAM.

Sandwich ELISA - Human FGF1 ELISA Kit, Chemiluminescent (AB327288)
  • sELISA

Supplier Data

Sandwich ELISA - Human FGF1 ELISA Kit, Chemiluminescent (AB327288)

Interpolated concentrations of native FGF1 in human mouse and rat brain samples based on 40 µg/mL 20 µg/mL and 20 µg/mL extract loads respectively.

The concentrations of FGF1 were measured in duplicate and interpolated from the FGF1 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD n=2). The mean FGF1 concentration was determined to be 886 pg/mL in human brain extract 179 pg/mL in mouse brain extract and 68 pg/mL in rat brain extract.

Sandwich ELISA - Human FGF1 ELISA Kit, Chemiluminescent (AB327288)
  • sELISA

Lab

Sandwich ELISA - Human FGF1 ELISA Kit, Chemiluminescent (AB327288)

Standard curve comparison between the Chemiluminescent Human FGF1 SimpleStep ELISA and colorimetric Human FGF1 SimpleStep ELISA (ab219636) in Sample Diluent NS. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human FGF1 ELISA Kit, Chemiluminescent (AB327288)
  • sELISA

Lab

Sandwich ELISA - Human FGF1 ELISA Kit, Chemiluminescent (AB327288)

Dynamic range comparison between the Chemiluminescent Human FGF1 SimpleStep ELISA, colorimetric Human FGF1 SimpleStep ELISA (ab219636), and leading colorimetric competitor. The Chemiluminescent SimpleStep ELISA kit shows increased sensitivity and increased dynamic range.

Sandwich ELISA - Human FGF1 ELISA Kit, Chemiluminescent (AB327288)
  • sELISA

Lab

Sandwich ELISA - Human FGF1 ELISA Kit, Chemiluminescent (AB327288)

Interpolated concentrations of spiked FGF1 in human serum, saliva, and stimulated PBMC supernatant samples. The concentrations of FGF1 were measured in duplicates, interpolated from the FGF1 standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 50%, saliva 50%, and stimulated PBMC supernatant 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean FGF1 concentration was determined to be 868 pg/mL in neat serum, 903 pg/mL in neat saliva, and 518 pg/mL in neat stimulated PBMC culture supernatant.

Key facts

Detection method

Luminescent

Sample types

Serum, Saliva, Tissue Extracts, Cell culture media

Reacts with

Human, Mouse, Rat

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 1.63 pg/mL

Range

3.43 - 2500 pg/mL

Assay time

55m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human FGF1 ELISA Kit, Chemiluminescent (ab327288) is a rapid single-wash sandwich 55-min ELISA to measure Human FGF1 in Serum, Saliva, Tissue Extracts, Cell culture media with a sensitivity of 1.63 pg/mL.

How the assay works

Human FGF1 ELISA Kit, Chemiluminescent (ab327288) SimpleStep Ignite™ ELISA employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step during a 30 min only incubation, significantly reducing assay time. By utilizing a chemiluminscent detection method this improves the sensitivity and dynamic range when compared to traditional colorimetric ELISAs.

Assay Specificity

Our SimpleStep Ignite™ ELISA kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human FGF1 (ab327288) protocol summary

1. Mix : add samples / standards to the wells together with the capture and detector antibody cocktail. Incubate 30 min at room temperature
2. Wash
3. Add ChemiHRP development solution - incubate for 2 min
4. Read the results on a plate reader capable of luminescence detection

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-FGF1 antibody [EPR19989-72] - BSA and Azide free (Capture) ab242964
- Anti-FGF1 antibody [EPR19989-176] - BSA and Aside free (Detector) ab242998

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Extract", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.4" }, { "reproducibilityType": "Inter", "sample": "Extract", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.8" } ]
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Recovery

[ { "sample": "Serum", "range": "89 - 94 %", "average": "= 91" }, { "sample": "Saliva", "range": "87 - 91 %", "average": "= 88" }, { "sample": "Tissue Extracts", "range": "101 - 104 %", "average": "= 103" }, { "sample": "Cell culture media", "range": "112 - 115 %", "average": "= 114" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

Plays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. Functions as a potent mitogen in vitro. Acts as a ligand for FGFR1 and integrins. Binds to FGFR1 in the presence of heparin leading to FGFR1 dimerization and activation via sequential autophosphorylation on tyrosine residues which act as docking sites for interacting proteins, leading to the activation of several signaling cascades. Binds to integrin ITGAV : ITGB3. Its binding to integrin, subsequent ternary complex formation with integrin and FGFR1, and the recruitment of PTPN11 to the complex are essential for FGF1 signaling. Induces the phosphorylation and activation of FGFR1, FRS2, MAPK3/ERK1, MAPK1/ERK2 and AKT1 (PubMed : 18441324, PubMed : 20422052). Can induce angiogenesis (PubMed : 23469107).
See full target information FGF1

Alternative Names

FGFA, FGF1, Fibroblast growth factor 1, FGF-1, Acidic fibroblast growth factor, Endothelial cell growth factor, Heparin-binding growth factor 1, aFGF, ECGF, HBGF-1
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websiteProtocolBooklet
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com