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AB313604

Human FGF12 ELISA Kit

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Human FGF12 ELISA Kit is a Sandwich ELISA for the measurement of Human FGF12 in Human in Cell Culture Media, Biofluids samples.
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Sandwich ELISA - Human FGF12 ELISA Kit (AB313604)
  • sELISA

Supplier Data

Sandwich ELISA - Human FGF12 ELISA Kit (AB313604)

Example of standard curve using ab313604. This standard curve is for demonstrative purposes only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Cell culture supernatant, Plasma, Serum

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 32 pg/mL

Range

32 - 8000 pg/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human FGF12 ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human FGF12 in serum, plasma and cell culture supernatants. This assay employs an antibody specific for human FGF12 coated on a 96-well plate.

This ELISA kit shows no cross-reactivity with the following cytokines tested: human ALK-1, B7-H2, BLAME, BMP-8, CD28, Common beta Chain (CD131), Contactin-1, Desmoglein-1, Desmoglein-3, EDAR, EphA1, EphB6, Ephrin-B3, Epiregulin, FGF-17, FOLR2, Galectin-8, Glypican 1, Glypican 5, GHR, IFNgamma R1, IL-22 R alpha 1, IL-22BP, IL-23 R, IL-31 RA, IL-7 R alpha, Integrin alpha 5, MDM2, Nectin-1, NKp30, Nogo Receptor, Notch-3, OSM R beta, Prolactin R, RELT, Ryk, Semaphorin 6D, Semaphorin 7A, Siglec-11.

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Precision & recovery information

Recovery

[ { "sample": "Serum", "range": "89 - 113 %", "average": "= 95.82" }, { "sample": "Plasma", "range": "75 - 103 %", "average": "= 86.11" }, { "sample": "Cell culture media", "range": "69 - 108 %", "average": "= 88.64" } ]

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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab313604-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB313604-CMP04", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Human FGF-12 Antibody-coated ELISA Plate", "number":"AB313604-CMP08", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution; 0.2M Sulfuric Acid", "number":"AB313604-CMP06", "productcode":"" }, { "size":"1 x 200 µL", "name":"300X HRP-Streptavidin", "number":"AB313604-CMP03", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Lyophilized Human FGF-12 Protein Standard", "number":"AB313604-CMP10", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB313604-CMP07", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent (Item E2)", "number":"AB313604-CMP05", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Human FGF-12 Detection Antibody", "number":"AB313604-CMP09", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
Please refer to protocols
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

FGF12 also known as fibroblast growth factor 12 is a member of the fibroblast growth factor family and has a molecular mass of approximately 28 kDa. FGF12 is expressed in various human tissues notably in the brain and heart where it plays an essential role in cellular processes. Unlike many other FGF family members FGF12 does not have a heparin-binding domain. This distinction affects its interaction with local extracellular matrices and influences how it functions within cells.
Biological function summary

Fibroblast growth factors regulate cell differentiation growth and tissue repair. Though not a typical secreted growth factor FGF12 functions intracellularly where it contributes to neuronal signaling. FGF12 interacts with ion channels and proteins in the central nervous system suggesting its role in modifying electrical signaling pathways. It does not typically form part of a large complex but it associates with proteins that mediate such signaling functions.

Pathways

FGF12 plays a significant role in the intracellular signaling pathways especially those related to neurogenesis and cardiac conduction. In these pathways FGF12 regulates ion channel activity ensuring proper signal propagation. It connects with related pathways of other fibroblast growth factors such as FGF11 sharing key similarities in modulating electrophysiological properties within excitable cells like neurons and cardiomyocytes.

FGF12 has links to cardiac arrhythmias and epilepsy. Alterations in FGF12 expression can disrupt normal cardiac and neural electrical activity hinting at its involvement in these conditions. It interacts with several ion channels such as the sodium channel subunit SCN5A which can result in clinical manifestations when dysregulated. Understanding FGF12's role provides insights into potential therapeutic targets for managing arrhythmias and seizure disorders.
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Product protocols

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Target data

Involved in nervous system development and function. Involved in the positive regulation of voltage-gated sodium channel activity. Promotes neuronal excitability by elevating the voltage dependence of neuronal sodium channel SCN8A fast inactivation.
See full target information FGF12

Alternative Names

FGF12B, FHF1, FGF12, Fibroblast growth factor 12, FGF-12, Fibroblast growth factor homologous factor 1, Myocyte-activating factor, FHF-1
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Product promise

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