Human FGF16 ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Human FGF16 in Human in Cell culture supernatant, Plasma, Serum samples.
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Key facts
- Detection method
- Colorimetric
- Sample types
- Cell culture supernatant, Plasma, Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 0.82 - 200 ng/mL
- Sensitivity
- = 0.82 ng/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Plays an important role in the regulation of embryonic development, cell proliferation and cell differentiation, and is required for normal cardiomyocyte proliferation and heart development.
Alternative names
Fibroblast growth factor 16, FGF-16, FGF16
What's included?
Human FGF16 ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Human FGF16 in Human in Cell culture supernatant, Plasma, Serum samples.
Key facts
- Detection method
- Colorimetric
- Sample types
- Cell culture supernatant, Plasma, Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 0.82 - 200 ng/mL
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 0.82 ng/mL
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Serum | Average % = 89.05 | Range 85 - 96 % |
Sample type Plasma | Average % = 92.03 | Range 68 - 119 % |
Sample type Cell culture media | Average % = 119.5 | Range 111 - 131 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- -20°C
Notes
Human FGF16 ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human FGF16 in serum, plasma and cell culture supernatants. This assay employs an antibody specific for human FGF16 coated on a 96-well plate.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Fibroblast Growth Factor 16 (FGF16) also known as FGF-16 is a protein belonging to the fibroblast growth factor family. It weighs approximately 27 kDa. FGF16 participates in cellular activities like proliferation and differentiation by binding to fibroblast growth factor receptors. Its expression appears primarily in the heart and skeletal muscles though it may also occur in other tissues under specific conditions.
- Biological function summary
FGF16 plays a significant role in tissue repair and development. It does not function alone; rather it acts as part of a signaling complex involving fibroblast growth factor receptors. This interaction is important for cardiac muscle development suggesting its importance in maintaining heart health. Furthermore FGF16 supports the healing processes in damaged muscle tissue contributing to tissue homeostasis.
- Pathways
FGF16 is active in both the PI3K-Akt signaling pathway and the MAPK signaling pathway. These pathways manage processes associated with cell survival growth and division. In these pathways FGF16 interacts with proteins like Akt (protein kinase B) and ERK (extracellular signal-regulated kinase) indicating its involvement in the regulation of cell cycle and cellular responses to external stimuli.
- Associated diseases and disorders
FGF16 is linked to cardiac hypertrophy and skeletal muscle-related conditions. Altered FGF16 expression can exacerbate heart muscle enlargement often leading to heart failure. ABI1 another protein associates with FGF16 in cellular signaling affected by these conditions. Moreover any disruption in FGF16's role in skeletal muscle repair may contribute to muscular dystrophies suggesting a potential target for therapeutic intervention.
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1 product image
Sandwich ELISA - Human FGF16 ELISA Kit (ab313605)
Example of standard curve using ab313605. This standard curve is for demonstrative purposes only. A standard curve must be run with each assay.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
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