AB230943

Human FGF19 ELISA Kit

Recombinant
SimpleStep
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(4 Publications)

Human FGF19 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human FGF19 with a sensitivity of 1.4 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

UNQ334/PRO533, FGF19, Fibroblast growth factor 19, FGF-19

6 Images

Sandwich ELISA - Human FGF19 ELISA Kit (AB230943)

sELISA

Supplier Data

Sandwich ELISA - Human FGF19 ELISA Kit (AB230943)

Interpolated concentrations of native FGF19 in human serum, and plasma samples.

The concentrations of FGF19 were measured in duplicates, interpolated from the FGF19 standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 12.5%, plasma (EDTA) 12.5%, plasma (heparin) 12.5% and plasma (citrate) 12.5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean FGF19 concentration was determined to be 153 pg/mL in serum , 91 pg/mL in plasma (EDTA), 187 pg/mL in plasma (heparin), and 182 pg/mL in plasma (citrate).

sELISA

Supplier Data

Sandwich ELISA - Human FGF19 ELISA Kit (AB230943)

Interpolated concentrations of native FGF19 in HT-29 cell culture supernatant.

The concentrations of FGF19 were measured in duplicates, interpolated from the FGF19 standard curves and corrected for sample dilution. Undiluted samples are as follows : HT-29 cell culture supernatant 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean FGF19 concentration was determined to be 741 pg/mL in HT-29 cell culture supernatant.

sELISA

Supplier Data

Sandwich ELISA - Human FGF19 ELISA Kit (AB230943)

Interpolated concentrations of spike FGF19 in human serum, and plasma samples.

sELISA

Supplier Data

Sandwich ELISA - Human FGF19 ELISA Kit (AB230943)

Human FGF19 Competitor Std Curve Comparison.

Standard curve comparison between human FGF19 SimpleStep ELISA^® kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.

sELISA

Supplier Data

Sandwich ELISA - Human FGF19 ELISA Kit (AB230943)

Serum from ten individual healthy human male donors was measured in duplicate.

Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean FGF19 concentration was determined to be 381 pg/mL with a range of 136 – 944 pg/mL.

sELISA

Supplier Data

Sandwich ELISA - Human FGF19 ELISA Kit (AB230943)

Example of human FGF19 standard curve in Sample Diluent NS.

Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 1.75 pg/mL

Range

3.91 - 250 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Human FGF19 ELISA Kit (ab230943) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of FGF19 protein in cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human FGF19 with 1.4 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Supernatant", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "6.6" }, { "reproducibilityType": "Intra", "sample": "Supernatant", "replicates": 5, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.8" } ]

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Recovery

[ { "sample": "Serum", "range": "106 - 117 %", "average": "= 112" }, { "sample": "Heparin Plasma", "range": "101 - 116 %", "average": "= 108" }, { "sample": "Citrate plasma", "range": "97 - 105 %", "average": "= 100" }, { "sample": "Cell culture media", "range": "107 - 110 %", "average": "= 108" }, { "sample": "EDTA Plasma", "range": "94 - 99 %", "average": null } ]

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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab230943-1x96Tests", "assetComponentDetails": [ { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB230943-CMP10", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB230943-CMP03", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB230943-CMP01", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB230943-CMP05", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB230943-CMP09", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BI", "number":"AB230943-CMP07", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human FGF19 Capture Antibody", "number":"AB230943-CMP08", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human FGF19 Lyophilized Recombinant Protein", "number":"AB230943-CMP06", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human FGF19 Detector Antibody", "number":"AB230943-CMP04", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB230943-CMP02", "productcode":"" } ] } } }

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

FGF19 also known as fibroblast growth factor 19 is a 251 amino acid protein with a molecular mass of approximately 27 kDa. It is mostly expressed in the liver small intestine and to some extent in the gallbladder. FGF19 is related to the FGF family of growth factors which are involved in a variety of developmental and physiological processes. Unlike many other FGF members FGF19 lacks a heparin-binding domain which influences its receptor binding characteristics. Researchers frequently use FGF19 ELISA kits and FGF19 assays to study this protein due to its significance in regulating bile acid metabolism.

Biological function summary

Fibroblast growth factor 19 participates in maintaining metabolic homeostasis and controlling bile acid synthesis. It acts by binding to a receptor complex composed of FGFR4 and beta-Klotho which signals the regulation of energy balance and interaction with other metabolic targets. FGF19 protein triggers pathways that modulate glucose and lipid metabolism making it an important factor in metabolic health. Its regulation of lipid and glucose processes emphasizes its role in metabolic control systems and potential impacts on obesity and diabetes management.

Pathways

The fibroblast growth factor 19 engages in two critical signaling routes: the MAPK/ERK and the PI3K/AKT pathways. These pathways are fundamental in cell proliferation differentiation and survival. FGF19 through FGFR4 activation influences regulatory proteins and factors downstream bridging interactions with other key molecules such as insulin and leptin pathways. This regulatory mechanism contributes to its ability to fine-tune a wide range of metabolic functions and influences energy balance.

Fibroblast growth factor 19 shows a significant connection to non-alcoholic fatty liver disease (NAFLD) and certain forms of cancer. Altered expression levels of FGF19 associate with liver conditions due to its role in bile acid regulation and energy homeostasis. In cancer particularly hepatocellular carcinoma the dysregulation of FGF19 and its receptor plays a part in unchecked cellular proliferation and tumorigenesis. Researchers have noted connections with proteins such as FGFR4 and beta-Klotho highlighting their collaborative roles in disease progression and potential therapeutic targeting strategies.

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Product protocols

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Target data

Involved in the suppression of bile acid biosynthesis through down-regulation of CYP7A1 expression, following positive regulation of the JNK and ERK1/2 cascades. Stimulates glucose uptake in adipocytes. Activity requires the presence of KLB and FGFR4.

See full target information FGF19

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Molecular metabolism 78:101823 PubMed37839774

2023

Changes in liver metabolic pathways demonstrate efficacy of the combined dietary and microbial therapeutic intervention in MASLD mouse model.

Applications

Unspecified application

Species

Unspecified reactive species

Valeria Iannone,Ambrin Farizah Babu,Johnson Lok,Carlos Gómez-Gallego,Giuseppe D'Auria,Ruben Vazquez-Uribe,Troels Holger Vaaben,Mareike Bongers,Santtu Mikkonen,Maija Vaittinen,Ida Tikkanen,Mikko Kettunen,Anton Klåvus,Ratika Sehgal,Dorota Kaminska,Jussi Pihlajamaki,Kati Hanhineva,Hani El-Nezami,Morten Otto Alexander Sommer,Marjukka Kolehmainen

Molecular and cellular endocrinology 570:111934 PubMed37085108

2023

Intestinal BMP-9 locally upregulates FGF19 and is down-regulated in obese patients with diabetes.

Applications

Unspecified application

Species

Unspecified reactive species

Stephan Drexler,Chen Cai,Anna-Lena Hartmann,Denise Moch,Haristi Gaitantzi,Theresa Ney,Malin Kraemer,Yuan Chu,Yuwei Zheng,Mohammad Rahbari,Annalena Treffs,Alena Reiser,Bénédicte Lenoir,Nektarios A Valous,Dirk Jäger,Emrullah Birgin,Tejas A Sawant,Qi Li,Keshu Xu,Lingyue Dong,Mirko Otto,Timo Itzel,Andreas Teufel,Norbert Gretz,Lukas J A C Hawinkels,Aránzazu Sánchez,Blanca Herrera,Rudolf Schubert,Han Moshage,Christoph Reissfelder,Matthias P A Ebert,Nuh N Rahbari,Katja Breitkopf-Heinlein

Science advances 9:eadf4490 PubMed37058568

2023

IL-6-GP130 signaling protects human hepatocytes against lipid droplet accumulation in humanized liver models.

Applications

Unspecified application

Species

Unspecified reactive species

Marisa Carbonaro,Kehui Wang,Hui Huang,Davor Frleta,Aditi Patel,Alexander Pennington,Mathieu Desclaux,Sven Moller-Tank,Justin Grindley,Judith Altarejos,Jun Zhong,Greg Polites,William Poueymirou,Stephen Jaspers,Christos Kyratsous,Brian Zambrowicz,Andrew Murphy,John C Lin,Lynn E Macdonald,Christopher Daly,Mark Sleeman,Gavin Thurston,Zhe Li

Nature communications 14:1305 PubMed36894566

2023

Gut microbiota alters host bile acid metabolism to contribute to intrahepatic cholestasis of pregnancy.

Applications

Unspecified application

Species

Unspecified reactive species

Bo Tang,Li Tang,Shengpeng Li,Shuang Liu,Jialin He,Pan Li,Sumin Wang,Min Yang,Longhui Zhang,Yuanyuan Lei,Dianji Tu,Xuefeng Tang,Hua Hu,Qin Ouyang,Xia Chen,Shiming Yang

View all publications

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websiteProtocolBooklet

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Human FGF19 ELISA Kit

Sandwich ELISA - Human FGF19 ELISA Kit (AB230943)

Sandwich ELISA - Human FGF19 ELISA Kit (AB230943)

Sandwich ELISA - Human FGF19 ELISA Kit (AB230943)

Sandwich ELISA - Human FGF19 ELISA Kit (AB230943)

Sandwich ELISA - Human FGF19 ELISA Kit (AB230943)

Sandwich ELISA - Human FGF19 ELISA Kit (AB230943)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay time

Assay Platform

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

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