Human FGF2 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human FGF2 with a sensitivity of 4 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
- Cited in over 10 citations
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- Nature communications 14:76392023Acoustofluidic Interfaces for the Mechanobiological Secretome of MSCs.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYe He,Shujie Yang,Pengzhan Liu,Ke Li,Ke Jin,Ryan Becker,Jinxin Zhang,Chuanchuan Lin,Jianping Xia,Zhehan Ma,Zhiteng Ma,Ruoyu Zhong,Luke P Lee,Tony Jun HuangPubMed 37993431
- International journal of molecular sciences 24:2023The Synergistic Effect of Electrical Stimulation and Dermal Fibroblast Cells-Laden 3D Conductive Hydrogel for Full-Thickness Wound Healing.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYen-Hong Lin,En-Wei Liu,Yun-Jhen Lin,Hooi Yee Ng,Jian-Jr Lee,Tuan-Ti HsuPubMed 37511457
- Regenerative biomaterials 10:rbad0582023Chitin nanocrystal-assisted 3D bioprinting of gelatin methacrylate scaffolds.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhengyun Ling,Jian Zhao,Shiyu Song,Shuwei Xiao,Pengchao Wang,Ziyan An,Zhouyang Fu,Jinpeng Shao,Zhuang Zhang,Weijun Fu,Shenghan SongPubMed 37359730
- ACS biomaterials science & engineering 9:719-7312023Degradable Biocompatible Porous Microtube Scaffold for Extended Donor Cell Survival and Activity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHelen Nguyen,Chien-Chung Chen,Andreas Czosseck,Max M Chen,Thomashire A George,David J LundyPubMed 36595653
- Nature methods 19:1449-14602022Geometric engineering of organoid culture for enhanced organogenesis in a dish.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSunghee Estelle Park,Shawn Kang,Jungwook Paek,Andrei Georgescu,Jeehan Chang,Alex Yoon Yi,Benjamin J Wilkins,Tatiana A Karakasheva,Kathryn E Hamilton,Dan Dongeun HuhPubMed 36280722
- American journal of translational research 14:6712-67252022Transplantation of bFGF-transfected bone mesenchymal stem cells on collagen scaffolds promotes the regeneration of injured rat endometrium.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFang Chen,Yingxin Gong,Ninghong Jiang,Jingjing Xiao,Yaping Wang,Limei Chen,Long SuiPubMed 36247308
- Cellular and molecular life sciences : CMLS 79:2942022Prenatal glucocorticoid exposure selectively impairs neuroligin 1-dependent neurogenesis by suppressing astrocytic FGF2-neuronal FGFR1 axis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGee Euhn Choi,Chang Woo Chae,Mo Ran Park,Jee Hyeon Yoon,Young Hyun Jung,Hyun Jik Lee,Ho Jae HanPubMed 35562616
- American journal of translational research 14:2637-26462022Keap1-Nrf2/ARE signal pathway activated by butylphthalide in the treatment of ischemic stroke.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiaofeng Zhang,Qiang Wu,Zhihui Wang,Haimei Li,Jie DaiPubMed 35559381
- Bioelectrochemistry (Amsterdam, Netherlands) 144:1079802021Gene electrotransfer of FGF2 enhances collagen scaffold biocompatibility.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCarly Boye,Kyle Christensen,Kamal Asadipour,Scott DeClemente,Michael Francis,Anna BulyshevaPubMed 34847373
- Biomedicines 9:2021Biofabrication of Gingival Fibroblast Cell-Laden Collagen/Strontium-Doped Calcium Silicate 3D-Printed Bi-Layered Scaffold for Osteoporotic Periodontal Regeneration.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChen-Ying Wang,Yung-Cheng Chiu,Alvin Kai-Xing Lee,Yun-An Lin,Ping-Yi Lin,Ming-You ShiePubMed 33923505
Key facts
- Detection method
- Colorimetric
- Sample types
- Cell culture extracts, Tissue Extracts, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 15.625 - 2500 pg/mL
- Assay time
- 1h 30m
- Sensitivity
- = 4 pg/mL
Abcam Recommends
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Acts as a ligand for FGFR1, FGFR2, FGFR3 and FGFR4 (PubMed:8663044). Also acts as an integrin ligand which is required for FGF2 signaling (PubMed:28302677). Binds to integrin ITGAV:ITGB3 (PubMed:28302677). Plays an important role in the regulation of cell survival, cell division, cell differentiation and cell migration (PubMed:28302677, PubMed:8663044). Functions as a potent mitogen in vitro (PubMed:1721615, PubMed:3732516, PubMed:3964259). Can induce angiogenesis (PubMed:23469107, PubMed:28302677). Mediates phosphorylation of ERK1/2 and thereby promotes retinal lens fiber differentiation (PubMed:29501879).
Alternative names
FGFB, FGF2, Fibroblast growth factor 2, FGF-2, Basic fibroblast growth factor, Heparin-binding growth factor 2, bFGF, HBGF-2
What's included?
Recommended products
Human FGF2 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human FGF2 with a sensitivity of 4 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
- Cited in over 10 citations
Key facts
- Detection method
- Colorimetric
- Sample types
- Cell culture extracts, Tissue Extracts, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 15.625 - 2500 pg/mL
- Assay time
- 1h 30m
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 4 pg/mL
Precision
Intra assay
| Sample | n | C.V. |
|---|---|---|
Sample Extract | n 8 | C.V. 3.7 |
Inter assay
| Sample | n | C.V. |
|---|---|---|
Sample Extract | n 3 | C.V. 1.8 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Cell culture supernatant | Average % = 95 | Range 84 - 104 % |
Sample type Serum | Average % = 103 | Range 101 - 106 % |
Sample type Cell culture extracts | Average % = 117 | Range 103 - 125 % |
Sample type Heparin Plasma | Average % = 95 | Range 89 - 105 % |
Sample type Citrate plasma | Average % = 95 | Range 92 - 100 % |
Sample type Cell culture media | Average % = 110 | Range 101 - 115 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Human FGF2 ELISA Kit ab246531 is a rapid single-wash 90-min Sandwich ELISA to measure Human FGF2 in cell culture extracts, cell culture supernatant, citrate plasma, heparin plasma, serum, tissue extracts. This SimpleStep sensitivity is 4 pg/mL.
How the assay works
Human FGF2 SimpleStep ELISA®employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.
Assay Specificity
Our SimpleStep ELISA® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.
Human FGF2 ELISA Kit ab246531 protocol summary
1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm
Design your own immunoassay
We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:
- Anti-FGF2 antibody [EPR20145-213] - BSA and Azide free (Capture) Anti-FGF2 antibody [EPR20145-7] - BSA and Azide free (Detector) ab259600
- Anti-FGF2 antibody [EPR20145-7] - BSA and Azide free (Detector) Anti-FGF2 antibody [EPR20145-7] - BSA and Azide free (Detector) ab259600
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
FGF2 also known as FGF-2 or basic fibroblast growth factor is a protein that plays important roles in various cellular processes. It is a member of the fibroblast growth factor family and has a molecular weight of approximately 18 kDa. Scientists find FGF2 protein in many tissues including brain myocardium and bone. The protein can exist in different forms including recombinant variants. It interacts with cell surface receptors to exert its effects significantly influencing cell behavior.
- Biological function summary
Various functions characterize FGF2 such as cell proliferation differentiation and migration. It does not act alone but is part of a larger protein complex. This complex includes FGF receptors which help mediate the binding of the FGF2 peptide ensuring proper signaling within the cells. FGF2 is important for development and repair processes making it an essential factor in tissue maintenance and regeneration.
- Pathways
FGF2 plays a big role in critical pathways like the MAPK/ERK signaling pathway and PI3K/Akt pathway. These pathways help regulate the processes of cell growth and survival. FGF2 interacts with other protein molecules such as FGFR1 and FGFR2 which serve as receptors to propagate the signaling cascade initiated by FGF2 binding. Its interaction in these pathways underlines its role in modulating important cellular responses.
- Associated diseases and disorders
Scientists connect FGF2 with conditions such as cancer and atherosclerosis. The protein's deregulation may lead to abnormal cell proliferation contributing to tumor growth and vascular diseases. FGF2's interaction with proteins like FGFR1 can influence cancer progression and plaque formation in blood vessels. By understanding FGF2's role in these diseases researchers aim to develop targeted therapies that can potentially alter disease outcomes.
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6 product images
ELISA - Human FGF2 ELISA Kit (ab246531)
Sandwich ELISA - Human FGF2 ELISA Kit (ab246531)
Example of human FGF2 standard curve in Sample Diluent NS.
The FGF2 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human FGF2 ELISA Kit (ab246531)
Example of human FGF2 standard curve in 1X Cell Extraction Buffer PTR.
The FGF2 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human FGF2 ELISA Kit (ab246531)
Example of human FGF2 standard curve in Sample Diluent 25BP.
The FGF2 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human FGF2 ELISA Kit (ab246531)
Interpolated concentrations of native FGF2 in K562 cell culture supernatant samples.
The concentrations of FGF2 were measured in duplicates, interpolated from the FGF2 standard curves and corrected for sample dilution. Undiluted samples are as follows: K562 supernatant 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean FGF2 concentration was determined to be 52.66 pg/mL in neat K562 cell culture supernatant.
Sandwich ELISA - Human FGF2 ELISA Kit (ab246531)
Interpolated concentrations of native FGF2 in HeLa cell extract based on a 25 μg/mL extract load.
The concentrations of FGF2 were measured in duplicate and interpolated from the FGF2 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean FGF2 concentration was determined to be 227.9 pg/mL in HeLa cell extract.
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