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AB246531

Human FGF2 ELISA Kit

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(1 Review)

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(13 Publications)

Human FGF2 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human FGF2 with a sensitivity of 4 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
- Cited in over 10 citations

View Alternative Names

FGFB, FGF2, Fibroblast growth factor 2, FGF-2, Basic fibroblast growth factor, Heparin-binding growth factor 2, bFGF, HBGF-2

6 Images
ELISA - Human FGF2 ELISA Kit (AB246531)
  • ELISA

Supplier Data

ELISA - Human FGF2 ELISA Kit (AB246531)
Sandwich ELISA - Human FGF2 ELISA Kit (AB246531)
  • sELISA

Supplier Data

Sandwich ELISA - Human FGF2 ELISA Kit (AB246531)

Example of human FGF2 standard curve in Sample Diluent NS.

The FGF2 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human FGF2 ELISA Kit (AB246531)
  • sELISA

Supplier Data

Sandwich ELISA - Human FGF2 ELISA Kit (AB246531)

Interpolated concentrations of native FGF2 in K562 cell culture supernatant samples.

The concentrations of FGF2 were measured in duplicates, interpolated from the FGF2 standard curves and corrected for sample dilution. Undiluted samples are as follows : K562 supernatant 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean FGF2 concentration was determined to be 52.66 pg/mL in neat K562 cell culture supernatant.

Sandwich ELISA - Human FGF2 ELISA Kit (AB246531)
  • sELISA

Supplier Data

Sandwich ELISA - Human FGF2 ELISA Kit (AB246531)

Interpolated concentrations of native FGF2 in HeLa cell extract based on a 25 μg/mL extract load.

The concentrations of FGF2 were measured in duplicate and interpolated from the FGF2 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean FGF2 concentration was determined to be 227.9 pg/mL in HeLa cell extract.

Sandwich ELISA - Human FGF2 ELISA Kit (AB246531)
  • sELISA

Supplier Data

Sandwich ELISA - Human FGF2 ELISA Kit (AB246531)

Example of human FGF2 standard curve in Sample Diluent 25BP.

The FGF2 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human FGF2 ELISA Kit (AB246531)
  • sELISA

Supplier Data

Sandwich ELISA - Human FGF2 ELISA Kit (AB246531)

Example of human FGF2 standard curve in 1X Cell Extraction Buffer PTR.

The FGF2 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Cell culture extracts, Tissue Extracts, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 4 pg/mL

Range

15.625 - 2500 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human FGF2 ELISA Kit ab246531 is a rapid single-wash 90-min Sandwich ELISA to measure Human FGF2 in cell culture extracts, cell culture supernatant, citrate plasma, heparin plasma, serum, tissue extracts. This SimpleStep sensitivity is 4 pg/mL.

How the assay works

Human FGF2 SimpleStep ELISA®employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human FGF2 ELISA Kit ab246531 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-FGF2 antibody [EPR20145-213] - BSA and Azide free (Capture) ab259599
- Anti-FGF2 antibody [EPR20145-7] - BSA and Azide free (Detector) ab259600

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Extract", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "1.8" }, { "reproducibilityType": "Intra", "sample": "Extract", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.7" } ]
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Recovery

[ { "sample": "Cell culture supernatant", "range": "84 - 104 %", "average": "= 95" }, { "sample": "Serum", "range": "101 - 106 %", "average": "= 103" }, { "sample": "Cell culture extracts", "range": "103 - 125 %", "average": "= 117" }, { "sample": "Heparin Plasma", "range": "89 - 105 %", "average": "= 95" }, { "sample": "Citrate plasma", "range": "92 - 100 %", "average": "= 95" }, { "sample": "Cell culture media", "range": "101 - 115 %", "average": "= 110" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

FGF2 also known as FGF-2 or basic fibroblast growth factor is a protein that plays important roles in various cellular processes. It is a member of the fibroblast growth factor family and has a molecular weight of approximately 18 kDa. Scientists find FGF2 protein in many tissues including brain myocardium and bone. The protein can exist in different forms including recombinant variants. It interacts with cell surface receptors to exert its effects significantly influencing cell behavior.
Biological function summary

Various functions characterize FGF2 such as cell proliferation differentiation and migration. It does not act alone but is part of a larger protein complex. This complex includes FGF receptors which help mediate the binding of the FGF2 peptide ensuring proper signaling within the cells. FGF2 is important for development and repair processes making it an essential factor in tissue maintenance and regeneration.

Pathways

FGF2 plays a big role in critical pathways like the MAPK/ERK signaling pathway and PI3K/Akt pathway. These pathways help regulate the processes of cell growth and survival. FGF2 interacts with other protein molecules such as FGFR1 and FGFR2 which serve as receptors to propagate the signaling cascade initiated by FGF2 binding. Its interaction in these pathways underlines its role in modulating important cellular responses.

Scientists connect FGF2 with conditions such as cancer and atherosclerosis. The protein's deregulation may lead to abnormal cell proliferation contributing to tumor growth and vascular diseases. FGF2's interaction with proteins like FGFR1 can influence cancer progression and plaque formation in blood vessels. By understanding FGF2's role in these diseases researchers aim to develop targeted therapies that can potentially alter disease outcomes.
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Product protocols

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Target data

Acts as a ligand for FGFR1, FGFR2, FGFR3 and FGFR4 (PubMed : 8663044). Also acts as an integrin ligand which is required for FGF2 signaling (PubMed : 28302677). Binds to integrin ITGAV : ITGB3 (PubMed : 28302677). Plays an important role in the regulation of cell survival, cell division, cell differentiation and cell migration (PubMed : 28302677, PubMed : 8663044). Functions as a potent mitogen in vitro (PubMed : 1721615, PubMed : 3732516, PubMed : 3964259). Can induce angiogenesis (PubMed : 23469107, PubMed : 28302677). Mediates phosphorylation of ERK1/2 and thereby promotes retinal lens fiber differentiation (PubMed : 29501879).
See full target information FGF2
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Publications (13)

Recent publications for all applications. Explore the full list and refine your search

Heliyon 10:e37364 PubMed39296104

2024

Insulin-like growth factor-2 mRNA-binding protein 2 facilitates post-ischemic angiogenesis by increasing the stability of fibroblast growth factor 2 mRNA and its protein expression.

Applications

Unspecified application

Species

Unspecified reactive species

Shuai Ma,Yiqing Hu,Wangguo Xu,Weidong Xiong,Xinyu Xu,Yajie Hou,Ying Wang,Panke Chen,Wenbi Yang,Hao Lu,Yongchao Zhao

Bioactive materials 37:439-458 PubMed38698918

2024

Facile and rapid fabrication of a novel 3D-printable, visible light-crosslinkable and bioactive polythiourethane for large-to-massive rotator cuff tendon repair.

Applications

Unspecified application

Species

Unspecified reactive species

Xu Zhang,Ke Li,Chenyang Wang,Ying Rao,Rocky S Tuan,Dan Michelle Wang,Dai Fei Elmer Ker

Nature communications 14:7639 PubMed37993431

2023

Acoustofluidic Interfaces for the Mechanobiological Secretome of MSCs.

Applications

Unspecified application

Species

Unspecified reactive species

Ye He,Shujie Yang,Pengzhan Liu,Ke Li,Ke Jin,Ryan Becker,Jinxin Zhang,Chuanchuan Lin,Jianping Xia,Zhehan Ma,Zhiteng Ma,Ruoyu Zhong,Luke P Lee,Tony Jun Huang

International journal of molecular sciences 24: PubMed37511457

2023

The Synergistic Effect of Electrical Stimulation and Dermal Fibroblast Cells-Laden 3D Conductive Hydrogel for Full-Thickness Wound Healing.

Applications

Unspecified application

Species

Unspecified reactive species

Yen-Hong Lin,En-Wei Liu,Yun-Jhen Lin,Hooi Yee Ng,Jian-Jr Lee,Tuan-Ti Hsu

Regenerative biomaterials 10:rbad058 PubMed37359730

2023

Chitin nanocrystal-assisted 3D bioprinting of gelatin methacrylate scaffolds.

Applications

Unspecified application

Species

Unspecified reactive species

Zhengyun Ling,Jian Zhao,Shiyu Song,Shuwei Xiao,Pengchao Wang,Ziyan An,Zhouyang Fu,Jinpeng Shao,Zhuang Zhang,Weijun Fu,Shenghan Song

ACS biomaterials science & engineering 9:719-731 PubMed36595653

2023

Degradable Biocompatible Porous Microtube Scaffold for Extended Donor Cell Survival and Activity.

Applications

Unspecified application

Species

Unspecified reactive species

Helen Nguyen,Chien-Chung Chen,Andreas Czosseck,Max M Chen,Thomashire A George,David J Lundy

Nature methods 19:1449-1460 PubMed36280722

2022

Geometric engineering of organoid culture for enhanced organogenesis in a dish.

Applications

Unspecified application

Species

Unspecified reactive species

Sunghee Estelle Park,Shawn Kang,Jungwook Paek,Andrei Georgescu,Jeehan Chang,Alex Yoon Yi,Benjamin J Wilkins,Tatiana A Karakasheva,Kathryn E Hamilton,Dan Dongeun Huh

American journal of translational research 14:6712-6725 PubMed36247308

2022

Transplantation of bFGF-transfected bone mesenchymal stem cells on collagen scaffolds promotes the regeneration of injured rat endometrium.

Applications

Unspecified application

Species

Unspecified reactive species

Fang Chen,Yingxin Gong,Ninghong Jiang,Jingjing Xiao,Yaping Wang,Limei Chen,Long Sui

Cellular and molecular life sciences : CMLS 79:294 PubMed35562616

2022

Prenatal glucocorticoid exposure selectively impairs neuroligin 1-dependent neurogenesis by suppressing astrocytic FGF2-neuronal FGFR1 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Gee Euhn Choi,Chang Woo Chae,Mo Ran Park,Jee Hyeon Yoon,Young Hyun Jung,Hyun Jik Lee,Ho Jae Han

American journal of translational research 14:2637-2646 PubMed35559381

2022

Keap1-Nrf2/ARE signal pathway activated by butylphthalide in the treatment of ischemic stroke.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaofeng Zhang,Qiang Wu,Zhihui Wang,Haimei Li,Jie Dai
View all publications
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