Human FGF21 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human FGF21 with a sensitivity of 3.3 pg/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 10x96 plates for bulk orders
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
- Cited in over 10 citations
View Alternative Names
UNQ3115/PRO10196, FGF21, Fibroblast growth factor 21, FGF-21
- ELISA
Supplier Data
ELISA - Human FGF21 ELISA Kit (AB222506)
- sELISA
Supplier Data
Sandwich ELISA - Human FGF21 ELISA Kit (AB222506)
Example of human FGF-21 standard curve in Sample Diluent NS.
Background-subtracted data values (mean +/- SD) are graphed
- sELISA
Supplier Data
Sandwich ELISA - Human FGF21 ELISA Kit (AB222506)
Neat serum samples from ten individual healthy human female donors was measured in duplicate.
Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean FGF-21 concentration was determined to be 413 pg/mL with a range of 59.4 – 1,537 pg/mL.
- sELISA
Supplier Data
Sandwich ELISA - Human FGF21 ELISA Kit (AB222506)
Interpolated concentrations of spiked FGF-21 in human breast milk samples.
The concentrations of FGF-21 was measured in duplicates, interpolated from the FGF-21 standard curves and corrected for sample dilution. Undiluted samples are as follows : milk 12.5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
- sELISA
Supplier Data
Sandwich ELISA - Human FGF21 ELISA Kit (AB222506)
Example of human FGF-21 standard curve in Sample Diluent NS. The FGF-21 standard curve was prepared as described. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
- sELISA
Supplier Data
Sandwich ELISA - Human FGF21 ELISA Kit (AB222506)
Interpolated concentrations of native FGF-21 in human serum and plasma samples.
The concentrations of FGF-21 were measured in duplicates, interpolated from the FGF-21 standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 100%, plasma (citrate) 100%, plasma (EDTA) 100%, and plasma (heparin) 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean FGF-21 concentration was determined to be 657 pg/mL in neat serum, 772 pg/mL in neat plasma (citrate), 289 pg/mL in neat plasma (EDTA), and 711 pg/mL in neat plasma (heparin).
- sELISA
Supplier Data
Sandwich ELISA - Human FGF21 ELISA Kit (AB222506)
Linearity of dilution is determined based on interpolated values from the standard curve. Linearity of dilution defines a sample concentration interval in which interpolated target concentrations are directly proportional to sample dilution.
Native human FGF-21 was measured in serum and plasma samples in a 2-fold dilution series. Sample dilutions are made in Sample Diluent NS for serum and plasma samples.
Recombinant human FGF-21 was spiked into EDTA plasma samples and diluted in a 2-fold dilution series in Sample Diluent NS.
- sELISA
Supplier Data
Sandwich ELISA - Human FGF21 ELISA Kit (AB222506)
Linearity of dilution.
Recombinant human FGF-21 was spiked into human milk sample and diluted in a 2-fold dilution series in Sample Diluent NS.
Reactivity data
Product details
Human FGF21 ELISA Kit ab222506 is a rapid single-wash 90-min Sandwich ELISA to measure Human FGF21 in citrate plasma, EDTA plasma, heparin plasma, milk, serum. This SimpleStep sensitivity is 3.3 pg/mL.
How the assay works
Human FGF21 SimpleStep ELISA®employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.
Assay Specificity
Our SimpleStep ELISA® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.
Human FGF21 ELISA Kit ab222506 protocol summary
1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm
Design your own immunoassay
We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:
- Anti-FGF21 antibody [EPR20586-230] - BSA and Azide free (Capture) ab244917
- Anti-FGF21 antibody [EPR20586-14] - BSA and Azide free (Detector) ab244917
FGF-21, or Fibroblast growth factor 21, is a member of the fibroblast growth factor family encoded by the gene FGF21. FGF-21 helps regulate glucose homeostasis by mediating liver signaling to the brain by inducing phosphorylation of the mitogen-activated protein kinase extracellular signal-related kinase 1. FGF-21 is principally expressed in the liver in response to fasting, where it mediates several metabolic responses such as ketogenesis, hepatic fatty acid oxidation, and resistance to growth hormones. In addition, it is involved with embryonic development, cell growth, morphogenesis, and tissue repair. Serum levels of FGF-21 have been found to be elevated in patients with type 2 diabetes mellitus, and higher levels of FGF-21 also correlate with higher liver fat content.
Precision
Recovery
What's included?
Properties and storage information
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
FGF21 influences glucose and lipid metabolism. It does not act as part of a larger protein complex. Instead it directly affects metabolic processes enhancing insulin sensitivity and lipid oxidation while reducing body weight. These actions help maintain energy balance and homeostasis making FGF21 an important player in metabolic control.
Pathways
FGF21 plays a significant role in the insulin signaling pathway and PPARγ coactivator 1α (PGC-1α) pathway. These pathways regulate cellular energy metabolism and mitochondrial biogenesis respectively. FGF21 interacts with other molecules such as PPARα (peroxisome proliferator-activated receptor alpha) to exert its effects demonstrating its integration into broader metabolic networks. FGF21's interaction with these pathways highlights its function in energy utilization and storage.
Product protocols
- Visit the General protocols
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Target data
Publications (10)
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Biomedicines 11: PubMed38137540
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Orphanet journal of rare diseases 18:367 PubMed38017461
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Nutrients 15: PubMed37686725
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Critical care (London, England) 27:196 PubMed37218012
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Journal of healthcare engineering 2022:6777771 PubMed35242298
2022
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Iranian journal of pharmaceutical research : IJPR 20:316-332 PubMed34567165
2021
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Frontiers in endocrinology 12:630287 PubMed33995273
2021
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BMJ open diabetes research & care 9: PubMed33789910
2021
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American journal of physiology. Cell physiology 320:C57-C65 PubMed33151090
2020
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Aging 11:7847-7858 PubMed31548436
2019
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Complementary Products
Primary Antibodies
AB171941
Anti-FGF21 antibody [EPR8314(2)]
RabMAb|Recombinant|KO Validated|20ul selling size
![Western blot - Anti-FGF21 antibody [EPR8314(2)] (AB171941)](https://content.abcam.com/products/images/fgf21-antibody-epr83142-ab171941--western-blot-img437869.jpg)
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Product promise
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