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AB325729

Human FGF21 ELISA Kit, Chemiluminescent

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Human FGF21 ELISA Kit, Chemiluminescent is a 55 min single-wash SimpleStep Ignite™ ELISA for the rapid quantification of Human FGF21 with higher sensitivity and broader measuring range.

- Single 30 min incubation and single -wash step for results in less than an hour
- Chemiluminescent detection allows a broad dynamic range : 2.29 - 5000 pg/ml with a sensitivity of 1.9 pg/mL
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
- Patent pending

View Alternative Names

UNQ3115/PRO10196, FGF21, Fibroblast growth factor 21, FGF-21

5 Images
Sandwich ELISA - Human FGF21 ELISA Kit, Chemiluminescent (AB325729)
  • sELISA

Lab

Sandwich ELISA - Human FGF21 ELISA Kit, Chemiluminescent (AB325729)

SimpleStep ELISA® Ignite technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 55 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

Sandwich ELISA - Human FGF21 ELISA Kit, Chemiluminescent (AB325729)
  • sELISA

Lab

Sandwich ELISA - Human FGF21 ELISA Kit, Chemiluminescent (AB325729)

Standard curve comparison between the Chemiluminescent Human FGF21 SimpleStep ELISA and colorimetric Human FGF21 SimpleStep ELISA (ab222506) in Sample Diluent NS. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human FGF21 ELISA Kit, Chemiluminescent (AB325729)
  • sELISA

Lab

Sandwich ELISA - Human FGF21 ELISA Kit, Chemiluminescent (AB325729)

Dynamic range comparison between the Chemiluminescent Human FGF21 SimpleStep ELISA, colorimetric Human FGF21 SimpleStep ELISA (ab222506), and leading colorimetric competitor. The Chemiluminescent SimpleStep ELISA kit shows increased range and sensitivity.

Sandwich ELISA - Human FGF21 ELISA Kit, Chemiluminescent (AB325729)
  • sELISA

Lab

Sandwich ELISA - Human FGF21 ELISA Kit, Chemiluminescent (AB325729)

Interpolated concentrations of native FGF21 in human serum and plasma samples. The concentrations of FGF21 were measured in duplicates, interpolated from the FGF21 standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 100%, plasma (citrate) 100%, plasma (EDTA) 100%, and plasma (heparin) 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean FGF21 concentration was determined to be 657 pg/mL in neat serum, 772 pg/mL in neat plasma (citrate), 289 pg/mL in neat plasma (EDTA), and 711 pg/mL in neat plasma (heparin).

Sandwich ELISA - Human FGF21 ELISA Kit, Chemiluminescent (AB325729)
  • sELISA

Lab

Sandwich ELISA - Human FGF21 ELISA Kit, Chemiluminescent (AB325729)

Neat serum samples from ten individual healthy human female donors were measured in duplicate. Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean FGF21 concentration was determined to be 413 pg/mL with a range of 59.4 – 1,537 pg/mL.

Key facts

Detection method

Luminescent

Sample types

Serum, Citrate plasma, EDTA Plasma, Heparin Plasma, Milk

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 1.9 pg/mL

Range

2.29 - 5000 pg/mL

Assay time

55m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

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Product details

Human FGF21 ELISA Kit, Chemiluminescent (ab325729) is a rapid single-wash sandwich 55 min ELISA to measure Human FGF21 in Serum, Citrate Plasma, EDTA Plasma, Heparin Plasma, Milk with a sensitivity of 1.9 pg/mL.

How the assay works

Human FGF21 ELISA Kit, Chemiluminescent (ab325729) SimpleStep Ignite™ ELISA employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step during a 30 min only incubation, significantly reducing assay time. By utilizing a chemiluminscent detection method this improves the sensitivity and dynamic range when compared to traditional colormetric ELISAs.

Assay Specificity

Our SimpleStep Ignite™ ELISA kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human FGF21 (ab325729) protocol summary

1. Mix : add samples / standards to the wells together with the capture and detector antibody cocktail. Incubate 30 min at room temperature
2. Wash
3. Add ChemiHRP development solution - incubate for 2 min
4. Read the results on a plate reader capable of luminescence detection

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-FGF21 antibody [EPR20586-230] - BSA and Azide free (Capture) ab244655
- Anti-FGF21 antibody [EPR20586-14] - BSA and Azide free (Detector) ab244917
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Precision

[ { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 5.3" }, { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 5.5" } ]
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Recovery

[ { "sample": "Serum", "range": "86 - 104 %", "average": "= 93" }, { "sample": "Citrate plasma", "range": "72 - 77 %", "average": "= 74" }, { "sample": "EDTA Plasma", "range": "85 - 97 %", "average": "= 92" }, { "sample": "Heparin Plasma", "range": "90 - 93 %", "average": "= 91" }, { "sample": "Milk", "range": "107 - 110 %", "average": "= 108" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

Stimulates glucose uptake in differentiated adipocytes via the induction of glucose transporter SLC2A1/GLUT1 expression (but not SLC2A4/GLUT4 expression). Activity requires the presence of KLB. Regulates systemic glucose homeostasis and insulin sensitivity.
See full target information FGF21
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websiteProtocolBooklet
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com