AB256399

Human FGFR-4 ELISA Kit

Recombinant
SimpleStep
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Human FGFR-4 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human FGFR-4 with a sensitivity of 15 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

CD334, JTK2, TKF, FGFR4, Fibroblast growth factor receptor 4, FGFR-4

4 Images

Sandwich ELISA - Human FGFR-4 ELISA Kit (AB256399)

sELISA

Supplier Data

Sandwich ELISA - Human FGFR-4 ELISA Kit (AB256399)

Interpolated concentrations of native FGFR-4 in 250 µg/mL HepG2 cell extract.

The concentrations of FGFR-4 were measured in duplicate and interpolated from the FGFR-4 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean FGFR-4 concentration was determined to be 6,588 pg/mL in 250 μg/mL HepG2 cell extract.

sELISA

Supplier Data

Sandwich ELISA - Human FGFR-4 ELISA Kit (AB256399)

Serum from ten individual healthy human male donors was measured in duplicate.

Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean FGFR-4 concentration was determined to be 8,757 pg/mL with a range of 3,634 – 33,733 pg/mL.

sELISA

Supplier Data

Sandwich ELISA - Human FGFR-4 ELISA Kit (AB256399)

Interpolated concentrations of native FGFR-4 in human serum, plasma and cell culture supernatant samples.

The concentrations of FGFR-4 were measured in duplicates, interpolated from the FGFR-4 standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 25%, plasma (EDTA) 12.5% and HepG2 cell culture supernatant 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean FGFR-4 concentration was determined to be 11,887 pg/mL in neat serum, 27,375 pg/mL in neat plasma (EDTA) and 11,607 pg/mL in neat HepG2 cell culture supernatant.

sELISA

Supplier Data

Sandwich ELISA - Human FGFR-4 ELISA Kit (AB256399)

Example of human FGFR-4 standard curve in Sample Diluent NS.

The FGFR-4 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Cell culture media, Cell culture supernatant, Serum, Cell Lysate, EDTA Plasma

Reacts with

Human, Macaque monkey

Assay type

Sandwich (quantitative)

Sensitivity

= 15 pg/mL

Range

62.5 - 4000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Human FGFR-4 ELISA Kit (ab256399) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of FGFR-4 protein in cell culture supernatant, cell lysate, edta plasma, serum, and cell culture media. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human FGFR-4 with 15 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

FGFR-4 (Fibroblast growth factor receptor 4) is a tyrosine-protein kinase that acts as cell-surface receptor for fibroblast growth factors and plays a role in the regulation of cell proliferation, differentiation and migration, and in regulation of lipid metabolism, bile acid biosynthesis, glucose uptake, vitamin D metabolism and phosphate homeostasis. Binding of FGF family members together with heparan sulfate proteoglycan or heparin promotes receptor dimerization and autophosphorylation on tyrosine residues. FGFR-4 mutations that lead to constitutive kinase activation or impair normal FGFR-4 inactivation lead to aberrant signaling. FGFR-4 is highly homologous among the species, rhesus macaque FGFR-4 is 97% identical on amino acid level.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.8" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.7" } ]

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Recovery

[ { "sample": "Cell culture supernatant", "range": "104 - 115 %", "average": "= 109" }, { "sample": "Serum", "range": "115 - 119 %", "average": "= 116" }, { "sample": "EDTA Plasma", "range": "109 - 121 %", "average": "= 114" }, { "sample": "Cell culture media", "range": "83 - 93 %", "average": "= 86" }, { "sample": "Cell Lysate", "range": "143 - 156 %", "average": "= 151" } ]

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What's included?

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

FGFR4 or fibroblast growth factor receptor 4 is a receptor tyrosine kinase weighing approximately 110 kDa. This protein plays a role in cellular processes by binding to fibroblast growth factors. FGFR4 is expressed in many tissues including muscle liver and the developing epidermis. It is known by alternate names like CD334 or FRFR4. This receptor's activation leads to receptor dimerization and autophosphorylation triggering downstream signaling pathways that affect cell proliferation differentiation and migration.

Biological function summary

FGFR4 influences various developmental and metabolic processes. It is important in limb and skeletal development epithelial cell morphogenesis and muscle fiber regeneration. FGFR4 often partners with heparan sulfate proteoglycans to form a signaling complex which defines the specificity and regulation of fibroblast growth factor signaling. The receptor's interaction with other proteins like FRFR4 and 5b5 further modulates its biological activity.

Pathways

FGFR4 participates in the MAPK and PI3K-Akt signaling pathways both important for regulating cell survival and growth. These pathways allow FGFR4 to connect with several other proteins such as FGFR1 and BLU-554. FGFR4's activation within these pathways helps determine cellular responses to hormonal and mitogenic signals further influencing tissue homeostasis and repair mechanisms.

FGFR4 is linked to certain cancers including hepatocellular carcinoma and colorectal cancer notable in cell lines like HCT116 and others. Overexpression or mutations in FGFR4 can drive tumor progression promoting uncontrolled growth and metastasis. FGFR4's relation to other key proteins such as FRAF and 19h3 highlights its role in pathogenesis making it a potential target for therapeutic interventions. Studies involving FGFR4 ELISA kits enhance understanding of its role in disease development and progression.

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Product protocols

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Target data

Tyrosine-protein kinase that acts as a cell-surface receptor for fibroblast growth factors and plays a role in the regulation of cell proliferation, differentiation and migration, and in regulation of lipid metabolism, bile acid biosynthesis, glucose uptake, vitamin D metabolism and phosphate homeostasis. Required for normal down-regulation of the expression of CYP7A1, the rate-limiting enzyme in bile acid synthesis, in response to FGF19. Phosphorylates PLCG1 and FRS2. Ligand binding leads to the activation of several signaling cascades. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. Phosphorylation of FRS2 triggers recruitment of GRB2, GAB1, PIK3R1 and SOS1, and mediates activation of RAS, MAPK1/ERK2, MAPK3/ERK1 and the MAP kinase signaling pathway, as well as of the AKT1 signaling pathway. Promotes SRC-dependent phosphorylation of the matrix protease MMP14 and its lysosomal degradation. FGFR4 signaling is down-regulated by receptor internalization and degradation; MMP14 promotes internalization and degradation of FGFR4. Mutations that lead to constitutive kinase activation or impair normal FGFR4 inactivation lead to aberrant signaling.

See full target information FGFR4

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websiteProtocolBooklet

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Human FGFR-4 ELISA Kit

Sandwich ELISA - Human FGFR-4 ELISA Kit (AB256399)

Sandwich ELISA - Human FGFR-4 ELISA Kit (AB256399)

Sandwich ELISA - Human FGFR-4 ELISA Kit (AB256399)

Sandwich ELISA - Human FGFR-4 ELISA Kit (AB256399)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay time

Assay Platform

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

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Primary Antibodies

Assay Kits

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Product promise