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AB309321

Human FGFR2 ELISA Kit - Extracellular

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Human FGFR2 ELISA Kit - Extracellular is a single-wash 90-min Simplestep used to quantify Human FGFR2 with a sensitivity of 5.241 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
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Sandwich ELISA - Human FGFR2 ELISA Kit - Extracellular (AB309321)
  • sELISA

Supplier Data

Sandwich ELISA - Human FGFR2 ELISA Kit - Extracellular (AB309321)

Relative reactivity of human FGFR2 isoforms beta IIIc, alpha IIIb and alpha IIIc

Interpolated concentration of FGFR2 was measured in duplicate at different sample concentrations. The samples were recombinant extracellular domains of human FGFR2 isoforms beta IIIc, alpha IIIb (dimer) and alpha IIIc. The interpolated, sample concentration-corrected values are plotted as percentage of beta IIIc

Sandwich ELISA - Human FGFR2 ELISA Kit - Extracellular (AB309321)
  • sELISA

Supplier Data

Sandwich ELISA - Human FGFR2 ELISA Kit - Extracellular (AB309321)

Interpolated concentrations of human FGFR2 in serum, plasma (citrate), plasma (EDTA), and plasma (heparin)

Interpolated concentration of native FGFR2 was measured in duplicate at different sample concentrations. Undiluted samples are 50% serum, plasma (citrate), plasma (EDTA), and plasma (heparin). The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

Sandwich ELISA - Human FGFR2 ELISA Kit - Extracellular (AB309321)
  • sELISA

Supplier Data

Sandwich ELISA - Human FGFR2 ELISA Kit - Extracellular (AB309321)

Interpolated concentrations of FGFR2 in normal human serum donors

Serum of nine individual healthy human female donors was measured in duplicate. Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean FGFR2 concentration was determined to be 2,291 pg/mL with a range of 1,609 – 3,089 pg/mL.

Sandwich ELISA - Human FGFR2 ELISA Kit - Extracellular (AB309321)
  • sELISA

Supplier Data

Sandwich ELISA - Human FGFR2 ELISA Kit - Extracellular (AB309321)

Example of human FGFR2 standard curve in Sample Diluent NS. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human FGFR2 ELISA Kit - Extracellular (AB309321)
  • sELISA

Supplier Data

Sandwich ELISA - Human FGFR2 ELISA Kit - Extracellular (AB309321)

Example of human FGFR2 standard curve. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Sample types

Serum, Heparin Plasma, Citrate plasma, EDTA Plasma, Cell culture media

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 5.241 pg/mL

Range

75 - 4800 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human FGFR2 - Extracellular SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of FGFR2 - Extracellular protein in human serum, plasma, saliva and cell culture supernatant. Quantitate Human FGFR2 - Extracellular with 5.241 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "6.4" }, { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.8" } ]
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Recovery

[ { "sample": "Serum", "range": "97 - 112 %", "average": "= 103" }, { "sample": "Heparin Plasma", "range": "86 - 110 %", "average": "= 96" }, { "sample": "Citrate plasma", "range": "92 - 111 %", "average": "= 104" }, { "sample": "EDTA Plasma", "range": "94 - 103 %", "average": "= 98" }, { "sample": "Cell culture media", "range": "102 - 107 %", "average": "= 105" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The fibroblast growth factor receptor 2 (FGFR2) also known as CD332 is a protein that functions as a transmembrane receptor tyrosine kinase. FGFR2 is important in signal transduction where it binds to fibroblast growth factors triggering downstream intracellular pathways. FGFR2 has two main isoforms FGFR2b and FGFR2c created through alternative splicing. The molecular weight of FGFR2 varies by isoform typically around 100-110 kDa. It gets expressed in various tissues including epithelial and mesenchymal tissues.
Biological function summary

FGFR2 plays a role in diverse cellular processes like proliferation differentiation and migration. As part of a receptor-ligand complex FGFR2 partners with fibroblast growth factors to initiate signaling cascades. It contributes to embryonic development tissue repair and angiogenesis. FGFR2b expression predominantly appears in epithelial cells while FGFR2c is found in mesenchymal cells reflecting its distinct role in tissue-specific signaling.

Pathways

FGFR2 integrates into key signaling networks especially the MAPK and PI3K/AKT pathways. It interacts with proteins like FRS2 and GRB2 which facilitate the activation of downstream signals promoting cell survival and growth. FGFR2's engagement in these pathways is critical for maintaining normal cellular functions and contributes to its involvement in regulating complex cellular responses.

FGFR2 mutations have connections with certain cancers and craniosynostosis syndromes. Alterations in FGFR2 signaling pathways can lead to aberrant cellular growth and have been found in various cancers including breast and gastric cancer. In craniosynostosis syndromes mutations in FGFR2 affect bone development linking it with disorders like Apert syndrome. These conditions demonstrate the pivotal role of FGFR2 in maintaining cellular homeostasis and the consequences of its dysfunction.
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Product protocols

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Target data

Tyrosine-protein kinase that acts as a cell-surface receptor for fibroblast growth factors and plays an essential role in the regulation of cell proliferation, differentiation, migration and apoptosis, and in the regulation of embryonic development. Required for normal embryonic patterning, trophoblast function, limb bud development, lung morphogenesis, osteogenesis and skin development. Plays an essential role in the regulation of osteoblast differentiation, proliferation and apoptosis, and is required for normal skeleton development. Promotes cell proliferation in keratinocytes and immature osteoblasts, but promotes apoptosis in differentiated osteoblasts. Phosphorylates PLCG1, FRS2 and PAK4. Ligand binding leads to the activation of several signaling cascades. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. Phosphorylation of FRS2 triggers recruitment of GRB2, GAB1, PIK3R1 and SOS1, and mediates activation of RAS, MAPK1/ERK2, MAPK3/ERK1 and the MAP kinase signaling pathway, as well as of the AKT1 signaling pathway. FGFR2 signaling is down-regulated by ubiquitination, internalization and degradation. Mutations that lead to constitutive kinase activation or impair normal FGFR2 maturation, internalization and degradation lead to aberrant signaling. Over-expressed FGFR2 promotes activation of STAT1.
See full target information FGFR2

Alternative Names

CD332, BEK, KGFR, KSAM, FGFR2, Fibroblast growth factor receptor 2, FGFR-2, K-sam, Keratinocyte growth factor receptor
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Product promise

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For full details, please see our Terms & Conditions

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