Human FGFR2 ELISA Kit - Intracellular is a single-wash 90-min Simplestep used to quantify Human FGFR2 with a sensitivity of 18.758 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
Images
Key facts
- Detection method
- Colorimetric
- Sample types
- Cell Lysate, Tissue Extracts
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 84.375 - 5400 pg/mL
- Assay time
- 1h 30m
- Sensitivity
- = 18.758 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Tyrosine-protein kinase that acts as a cell-surface receptor for fibroblast growth factors and plays an essential role in the regulation of cell proliferation, differentiation, migration and apoptosis, and in the regulation of embryonic development. Required for normal embryonic patterning, trophoblast function, limb bud development, lung morphogenesis, osteogenesis and skin development. Plays an essential role in the regulation of osteoblast differentiation, proliferation and apoptosis, and is required for normal skeleton development. Promotes cell proliferation in keratinocytes and immature osteoblasts, but promotes apoptosis in differentiated osteoblasts. Phosphorylates PLCG1, FRS2 and PAK4. Ligand binding leads to the activation of several signaling cascades. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. Phosphorylation of FRS2 triggers recruitment of GRB2, GAB1, PIK3R1 and SOS1, and mediates activation of RAS, MAPK1/ERK2, MAPK3/ERK1 and the MAP kinase signaling pathway, as well as of the AKT1 signaling pathway. FGFR2 signaling is down-regulated by ubiquitination, internalization and degradation. Mutations that lead to constitutive kinase activation or impair normal FGFR2 maturation, internalization and degradation lead to aberrant signaling. Over-expressed FGFR2 promotes activation of STAT1.
Alternative names
CD332, BEK, KGFR, KSAM, FGFR2, Fibroblast growth factor receptor 2, FGFR-2, K-sam, Keratinocyte growth factor receptor
What's included?
Recommended products
Human FGFR2 ELISA Kit - Intracellular is a single-wash 90-min Simplestep used to quantify Human FGFR2 with a sensitivity of 18.758 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
Key facts
- Detection method
- Colorimetric
- Sample types
- Cell Lysate, Tissue Extracts
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 84.375 - 5400 pg/mL
- Assay time
- 1h 30m
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 18.758 pg/mL
- General recovery
- 111%
Precision
Intra assay
| Sample | n | C.V. |
|---|---|---|
Sample Extract | n 8 | C.V. 7 |
Inter assay
| Sample | n | C.V. |
|---|---|---|
Sample Extract | n 3 | C.V. 5.5 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Cell Lysate | Average % = 111 | Range 96 - 122 pg/mL |
Sample type Tissue Extracts | Average % = 91 | Range 90 - 93 pg/mL |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Human FGFR2 - Intracellular SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of FGFR2 - Intracellular protein in human cell lysate and tissue extract samples. Quantitate Human FGFR2 - Intracellular with 18.758 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The fibroblast growth factor receptor 2 (FGFR2) also known as CD332 is a protein that functions as a transmembrane receptor tyrosine kinase. FGFR2 is important in signal transduction where it binds to fibroblast growth factors triggering downstream intracellular pathways. FGFR2 has two main isoforms FGFR2b and FGFR2c created through alternative splicing. The molecular weight of FGFR2 varies by isoform typically around 100-110 kDa. It gets expressed in various tissues including epithelial and mesenchymal tissues.
- Biological function summary
FGFR2 plays a role in diverse cellular processes like proliferation differentiation and migration. As part of a receptor-ligand complex FGFR2 partners with fibroblast growth factors to initiate signaling cascades. It contributes to embryonic development tissue repair and angiogenesis. FGFR2b expression predominantly appears in epithelial cells while FGFR2c is found in mesenchymal cells reflecting its distinct role in tissue-specific signaling.
- Pathways
FGFR2 integrates into key signaling networks especially the MAPK and PI3K/AKT pathways. It interacts with proteins like FRS2 and GRB2 which facilitate the activation of downstream signals promoting cell survival and growth. FGFR2's engagement in these pathways is critical for maintaining normal cellular functions and contributes to its involvement in regulating complex cellular responses.
- Associated diseases and disorders
FGFR2 mutations have connections with certain cancers and craniosynostosis syndromes. Alterations in FGFR2 signaling pathways can lead to aberrant cellular growth and have been found in various cancers including breast and gastric cancer. In craniosynostosis syndromes mutations in FGFR2 affect bone development linking it with disorders like Apert syndrome. These conditions demonstrate the pivotal role of FGFR2 in maintaining cellular homeostasis and the consequences of its dysfunction.
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3 product images
Sandwich ELISA - Human FGFR2 ELISA Kit - Intracellular (ab309322)
Example of human FGFR2 standard curve. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human FGFR2 ELISA Kit - Intracellular (ab309322)
Interpolated concentration of FGFR2 was measured in duplicate at different sample concentrations. The samples were recombinant extracellular domains of human FGFR2 isoforms beta IIIc, alpha IIIb (dimer) and alpha IIIc. The interpolated, sample concentration-corrected values are plotted as percentage of beta IIIc.
Sandwich ELISA - Human FGFR2 ELISA Kit - Intracellular (ab309322)
Interpolated concentration of native FGFR2 was measured in duplicate at different sample concentrations. Undiluted samples are 500 µg/mL Saos2 cell extract and 500 µg/mL MCF-7 cell extract. The interpolated dilution factor corrected values to undiluted are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer PTR.
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Product protocols
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