Human FGFR3 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human FGFR3 with a sensitivity of 61 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
Images
Key facts
- Detection method
- Colorimetric
- Sample types
- Cell culture extracts, Tissue Extracts
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 0.312 - 20 ng/mL
- Assay time
- 1h 30m
- Sensitivity
- = 61 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Tyrosine-protein kinase that acts as a cell-surface receptor for fibroblast growth factors and plays an essential role in the regulation of cell proliferation, differentiation and apoptosis. Plays an essential role in the regulation of chondrocyte differentiation, proliferation and apoptosis, and is required for normal skeleton development. Regulates both osteogenesis and postnatal bone mineralization by osteoblasts. Promotes apoptosis in chondrocytes, but can also promote cancer cell proliferation. Required for normal development of the inner ear. Phosphorylates PLCG1, CBL and FRS2. Ligand binding leads to the activation of several signaling cascades. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. Phosphorylation of FRS2 triggers recruitment of GRB2, GAB1, PIK3R1 and SOS1, and mediates activation of RAS, MAPK1/ERK2, MAPK3/ERK1 and the MAP kinase signaling pathway, as well as of the AKT1 signaling pathway. Plays a role in the regulation of vitamin D metabolism. Mutations that lead to constitutive kinase activation or impair normal FGFR3 maturation, internalization and degradation lead to aberrant signaling. Over-expressed or constitutively activated FGFR3 promotes activation of PTPN11/SHP2, STAT1, STAT5A and STAT5B. Secreted isoform 3 retains its capacity to bind FGF1 and FGF2 and hence may interfere with FGF signaling.
Alternative names
CD333, JTK4, FGFR3, Fibroblast growth factor receptor 3, FGFR-3
What's included?
Recommended products
Human FGFR3 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human FGFR3 with a sensitivity of 61 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
Key facts
- Detection method
- Colorimetric
- Sample types
- Cell culture extracts, Tissue Extracts
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 0.312 - 20 ng/mL
- Assay time
- 1h 30m
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 61 pg/mL
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Overall | n 3 | mean - | SD - | C.V. 3 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Overall | n 5 | mean - | SD - | C.V. 7 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Cell culture extracts | Average % = 100 | Range 80 - 115 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Human FGFR3 ELISA Kit (ab214027) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of FGFR3 protein in cell culture extracts and tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human FGFR3 with 61 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
FGFR3 is a tyrosine-protein kinase that acts as cell-surface receptor for fibroblast growth factors and plays an essential role in the regulation of cell proliferation, differentiation and apoptosis. FGFR3 plays an essential role in the regulation of chondrocyte differentiation, proliferation and apoptosis, and is required for normal skeleton development. FGFR3 regulates both osteogenesis and postnatal bone mineralization by osteoblasts. FGFR3 promotes apoptosis in chondrocytes, but can also promote cancer cell proliferation. FGFR3 is required for normal development of the inner ear. FGFR3 interacts in vitro with FGF1, FGF2, FGF4, FGF6; FGF8, FGF9, FGF10, FGF17, FGF18, FGF19, FGF20 and FGF23. FGFR3 interacts with KLB. The affinity for fibroblast growth factors (FGFs) is increased by heparan sulfate glycosaminoglycans that function as co-receptors. Likewise, KLB increases the affinity for FGF19 and FGF21. FGFR3 interacts with PIK3R1, PLCG1, SOCS1 and SOCS3. FGFR3 isoform 3 forms disulfide-linked dimers. Ligand binding to FGFR3 leads to FGFR3 dimerization and to the activation of several signalling cascades. FGFR3 phosphorylates PLCG1, CBL and FRS2. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. Phosphorylation of FRS2 triggers recruitment of GRB2, GAB1, PIK3R1 and SOS1, and mediates activation of RAS, MAPK1/ERK2, MAPK3/ERK1 and the MAP kinase signaling pathway, as well as of the AKT1 signaling pathway. FGFR3 plays a role in the regulation of vitamin D metabolism. Mutations that lead to constitutive kinase activation or impair normal FGFR3 maturation, internalization and degradation lead to aberrant signaling. Over-expressed or constitutively activated FGFR3 promotes activation of PTPN11/SHP2, STAT1, STAT5A and STAT5B. Secreted isoform 3 of FGFR3 retains its capacity to bind FGF1 and FGF2 and hence may interfere with FGF signalling.
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Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
FGFR3 or fibroblast growth factor receptor 3 is a tyrosine kinase receptor with a molecular mass of approximately 95 kDa. This receptor also known as CD333 primarily interacts with fibroblast growth factors. It resides on the cell surface and is predominately expressed in bone cartilage and the brain. FGFR3 plays a major role in cell growth regulation and differentiation affecting how cells respond to external signals.
- Biological function summary
FGFR3 participates in important processes such as chondrogenesis and osteogenesis which are essential for bone growth and development. The receptor operates within a complex system involving interactions with ligand-induced dimerization and autophosphorylation. This phosphorylation event activates the receptor's intrinsic kinase activity further triggering downstream signaling pathways for cellular responses.
- Pathways
FGFR3 is involved with the MAPK/ERK and PI3K/AKT pathways which play significant roles in cell proliferation differentiation and survival. FGFR3 links closely with FGF ligands and similar receptor types such as FGFR1 and FGFR2 within these pathways. Differences in pathway activation can lead to diverse biological responses illustrating its pivotal role in maintaining cellular homeostasis.
- Associated diseases and disorders
FGFR3 mutations have significant links to skeletal disorders most notably achondroplasia characterized by short limbs and abnormal bone growth. FGFR3 also associates with bladder cancer where overactivity of the receptor leads to increased cell proliferation and survival. Mutations in FGFR3 often correlate with these conditions impacting signaling pathways and interacting with proteins such as PTPN11 highlighting its critical role in pathogenesis.
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3 product images
Sandwich ELISA - Human FGFR3 ELISA Kit (ab214027)
Example of human FGFR3 standard curve.
Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human FGFR3 ELISA Kit (ab214027)
Interpolated concentrations of FGFR3 in extract of HEK293T cells overexpressing FGFR3 samples and native FGFR3 in A549 cell extract samples.
HEK293T cells overexpressing FGFR3 samples based on a 2 μg/mL extract load and native FGFR3 in A549 cell extract samples based on a 300 μg/mL extract load. The concentrations of FGFR3 were measured in duplicates, interpolated from the FGFR3 standard curves and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Values for A549 cell extract at 8X and 16X diluted had O.D. values below the O.D. values of the MDD. The mean FGFR3 concentration was determined to be 14.2 ng/mL in extract of HEK293T cells overexpressing FGFR3 and 0.44 ng/mL in A549 cell extract.
Sandwich ELISA - Human FGFR3 ELISA Kit (ab214027)
Interpolated concentrations of FGFR3 in extracts of HEK293T cells overexpressing human FGFR3 and HEK293T cells transfected with the associated empty vector.
The concentrations of FGFR3 were measured in three different dilutions in duplicate and interpolated from the FGFR3 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted in ng FGFR3 per mg of extract (mean +/- SD, n=3). FGFR3 concentration was determined to be 7234 ng/mg in extract of HEK293T cells overexpressing FGFR3 and 56.9 ng/mg in the empty vector.
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