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AB284622

Human FGL1 ELISA Kit

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Human FGL1 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human FGL1 with a sensitivity of 21.73 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

HFREP1, FGL1, Fibrinogen-like protein 1, HP-041, Hepassocin, Hepatocyte-derived fibrinogen-related protein 1, Liver fibrinogen-related protein 1, HPS, HFREP-1, LFIRE-1

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ELISA - Human FGL1 ELISA Kit (AB284622)
  • ELISA

Supplier Data

ELISA - Human FGL1 ELISA Kit (AB284622)
Sandwich ELISA - Human FGL1 ELISA Kit (AB284622)
  • sELISA

Supplier Data

Sandwich ELISA - Human FGL1 ELISA Kit (AB284622)

Interpolated concentrations of human FGL1 in serum, plasma (citrate), plasma (EDTA), plasma (heparin), and HepG2 cell culture supernatant.

Interpolated concentration of native FGL1 was measured in duplicate at different sample concentrations. Undiluted samples are as follows : serum 1%, plasma (citrate) 0.5%, plasma (EDTA) 0.5%, plasma (heparin) 0.5% and HepG2 cell culture supernatant 0.5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

Sandwich ELISA - Human FGL1 ELISA Kit (AB284622)
  • sELISA

Supplier Data

Sandwich ELISA - Human FGL1 ELISA Kit (AB284622)

Example of human FGL1 standard curve in Sample Diluent NS.

Example of human FGL1 standard curve. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human FGL1 ELISA Kit (AB284622)
  • sELISA

Supplier Data

Sandwich ELISA - Human FGL1 ELISA Kit (AB284622)

Interpolated concentrations of human FGL1 in HepG2 cell extract, A549 cell extract, and human liver tissue extract.

Interpolated concentration of native FGL1 was measured in duplicate at different sample concentrations. Undiluted samples are as follows : HepG2 cell extract 125 μg/mL, A549 cell extract 500 μg/mL, and human liver tissue extract 500 μg/mL. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer PTR.

Sandwich ELISA - Human FGL1 ELISA Kit (AB284622)
  • sELISA

Supplier Data

Sandwich ELISA - Human FGL1 ELISA Kit (AB284622)

Example of human FGL1 standard curve in 1X Cell Extraction Buffer PTR.

Example of human FGL1 standard curve. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, Cell Lysate, EDTA Plasma

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 21.73 pg/mL

Range

46.88 - 3000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human FGL1 ELISA Kit ab284622 is a rapid single-wash 90-min Sandwich ELISA to measure Human FGL1 in cell culture supernatant, cell lysate, citrate plasma, EDTA plasma, heparin plasma, serum. This SimpleStep sensitivity is 21.73 pg/mL.

How the assay works

Human FGL1 SimpleStep ELISA®employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human FGL1 ELISA Kit ab284622 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-FGL1 antibody [EPR24018-140] - BSA and Azide free (Capture) ab276021
- Anti-FGL1 antibody [EPR24018-18] - BSA and Azide free (Detector) ab276021
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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.5" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.3" } ]
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Recovery

[ { "sample": "Cell culture supernatant", "range": "96 - 118 %", "average": "= 105" }, { "sample": "Serum", "range": "95 - 117 %", "average": "= 106" }, { "sample": "EDTA Plasma", "range": "102 - 113 %", "average": "= 108" }, { "sample": "Heparin Plasma", "range": "92 - 99 %", "average": "= 95" }, { "sample": "Cell Lysate", "range": "95 - 104 %", "average": "= 96" }, { "sample": "Citrate plasma", "range": "87 - 103 %", "average": "= 96" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Fibrinogen-like protein 1 (FGL1) also known as Hepassocin plays a significant role in modulating immune responses. FGL1 is a member of the fibrinogen family with a molecular mass of approximately 31 kDa. This protein is widely expressed in the liver and is also found in other tissues like the pancreas and lungs. Its structural homology to fibrinogen suggests potential binding capabilities with similar ligands influencing its mechanical actions in cellular processes.
Biological function summary

FGL1 modulates the immune system and maintains its homeostasis. It functions primarily as a ligand for the immune checkpoint receptor LAG-3. This FGL1-LAG-3 interaction inhibits T-cell activity leading to immune tolerance and helping prevent autoimmunity. FGL1 while not a part of a complex demonstrates significant standalone biological activity by affecting immune responses especially under pathological conditions.

Pathways

FGL1 integrates into the immune regulatory framework. It exerts its effects mainly through the immune checkpoint pathway impacting T-cell regulation. In addition to LAG-3 FGL1 interacts closely with the PD-1/PD-L1 pathway which is also important in checkpoint inhibition and immune evasion mechanisms. These pathways together orchestrate the immune surveillance system and play roles in maintaining immune equilibrium.

FGL1 has received attention in cancer particularly in its role of suppressing anti-tumor immunity. Its interaction with LAG-3 makes it a potential target for cancer immunotherapy as high FGL1 levels correlate with poor prognosis in various cancers. Additionally it has implications in autoimmune diseases where its regulation of T-cell activity prevents excessive immune responses. By modulating LAG-3 activity FGL1 emerges as a therapeutic target of interest in autoimmune conditions presenting new opportunities for managing such disorders.
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Product protocols

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Target data

Immune suppressive molecule that inhibits antigen-specific T-cell activation by acting as a major ligand of LAG3 (PubMed : 30580966). Responsible for LAG3 T-cell inhibitory function (PubMed : 30580966). Binds LAG3 independently from MHC class II (MHC-II) (PubMed : 30580966). Secreted by, and promotes growth of, hepatocytes (PubMed : 11470158, PubMed : 19880967).
See full target information FGL1
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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