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AB325370

Human FGL1 ELISA Kit, Chemiluminescent

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Human FGL1 ELISA Kit, Chemiluminescent is a 55-min single-wash Simplestep IgniteTM ELISA for the rapid quantification of Human FGL1 with higher sensitivity and broader measuring range.

- Single 30 min incubation and single -wash step for results in less than an hour
- Chemiluminescent detection allows a broad dynamic range :4.6 - 10000 pg/mL with a sensitivity of 1.7 pg/mL
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
- Patent Pending

View Alternative Names

HFREP1, FGL1, Fibrinogen-like protein 1, HP-041, Hepassocin, Hepatocyte-derived fibrinogen-related protein 1, Liver fibrinogen-related protein 1, HPS, HFREP-1, LFIRE-1

5 Images
Sandwich ELISA - Human FGL1 ELISA Kit, Chemiluminescent (AB325370)
  • sELISA

Lab

Sandwich ELISA - Human FGL1 ELISA Kit, Chemiluminescent (AB325370)

SimpleStep ELISA® Ignite technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 55 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

Sandwich ELISA - Human FGL1 ELISA Kit, Chemiluminescent (AB325370)
  • sELISA

Lab

Sandwich ELISA - Human FGL1 ELISA Kit, Chemiluminescent (AB325370)

Dynamic range comparison between the Chemiluminescent Human FGL1 SimpleStep ELISA, colorimetric Human FGL1 SimpleStep ELISA (ab284622), and leading colorimetric competitor. The Chemiluminescent SimpleStep ELISA kit shows increased sensitivity.

Sandwich ELISA - Human FGL1 ELISA Kit, Chemiluminescent (AB325370)
  • sELISA

Lab

Sandwich ELISA - Human FGL1 ELISA Kit, Chemiluminescent (AB325370)

Standard curve comparison between the Chemiluminescent Human FGL1 SimpleStep ELISA and colorimetric Human FGL1 SimpleStep ELISA (ab284622) in Sample Diluent NS. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human FGL1 ELISA Kit, Chemiluminescent (AB325370)
  • sELISA

Lab

Sandwich ELISA - Human FGL1 ELISA Kit, Chemiluminescent (AB325370)

Serum of ten individual healthy human male donors was measured in duplicate. Interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). The mean FGL1 concentration was determined to be 122 ng/mL with a range of 26.3 – 338.1 ng/mL.

Sandwich ELISA - Human FGL1 ELISA Kit, Chemiluminescent (AB325370)
  • sELISA

Lab

Sandwich ELISA - Human FGL1 ELISA Kit, Chemiluminescent (AB325370)

Interpolated concentration of native FGL1 was measured in duplicate at different sample concentrations. Undiluted samples are as follows : serum 1%, plasma (citrate) 0.5%, plasma (EDTA) 0.5%, plasma (heparin) 0.5%, and HepG2 supernatant 0.5%. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

Key facts

Detection method

Luminescent

Sample types

Serum, Citrate plasma, EDTA Plasma, Heparin Plasma, Supernatant

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 1.7 pg/mL

Range

4.6 - 10000 pg/mL

Assay time

55m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human FGL1 ELISA Kit, Chemiluminescent (ab325370) is a rapid single-wash sandwich 55-min ELISA to measure Human FGL1 in Serum, Citrate Plasma, EDTA Plasma, Heparin Plasma, Supernatant with a sensitivity of 1.7 pg/ml.

How the assay works

Human FGL1 ELISA Kit, Chemiluminescent (ab325370) SimpleStep ELISA®  employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step during a 30 min only incubation, significantly reducing assay time.  By utilizing a chemiluminscent detection method this improves the sensitivity and dynamic range when compared to traditional colormetric ELISAs.

Assay Specificity

Our SimpleStep ELISA® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.

Please refer to our protocol booklet for more details.

Human FGL1 (ab325370) protocol summary

1. Mix : add samples / standards to the wells together with the capture and detector antibody cocktail. Incubate 30 min at room temperature

2. Wash

3. Add prepared ChemiHRP development solution - incubate for 2 min

4. Read the results on a plate reader capable of luminescence detection

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Rabbit monoclonal [EPR24018-140] to FGL1 - BSA and Azide free (Capture) ab276020

- Rabbit monoclonal [EPR24018-18] to FGL1 - BSA and Azide free (Detector) ab276021

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Precision

[ { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.3" }, { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.5" } ]
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Recovery

[ { "sample": "Serum", "range": "95 - 117 %", "average": "= 106" }, { "sample": "Citrate plasma", "range": "87 - 103 %", "average": "= 96" }, { "sample": "EDTA Plasma", "range": "102 - 113 %", "average": "= 108" }, { "sample": "Heparin Plasma", "range": "92 - 99 %", "average": "= 95" }, { "sample": "Cell culture supernatant", "range": "96 - 118 %", "average": "= 105" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab325370-1x96Tests", "assetComponentDetails": [ { "size":"1 x 3 mL", "name":"ChemiHRP Reagent A", "number":"AB325370-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated Black 96-Well Microplate", "number":"AB325370-CMP09", "productcode":"" }, { "size":"1 x 3 mL", "name":"ChemiHRP Reagent B", "number":"AB325370-CMP08", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 5BI", "number":"AB325370-CMP04", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB325370-CMP06", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB325370-CMP05", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human FGL1 Detector Antibody", "number":"AB325370-CMP02", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human FGL1 Capture Antibody", "number":"AB325370-CMP01", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seal", "number":"AB325370-CMP10", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human FGL1 Lyophilized Recombinant Protein", "number":"AB325370-CMP03", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

Immune suppressive molecule that inhibits antigen-specific T-cell activation by acting as a major ligand of LAG3 (PubMed : 30580966). Responsible for LAG3 T-cell inhibitory function (PubMed : 30580966). Binds LAG3 independently from MHC class II (MHC-II) (PubMed : 30580966). Secreted by, and promotes growth of, hepatocytes (PubMed : 11470158, PubMed : 19880967).
See full target information FGL1
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websiteProtocolBooklet
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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