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AB108841

Human Fibrinogen ELISA Kit

5

(1 Review)

|

(9 Publications)

Human Fibrinogen ELISA Kit is a sandwich ELISA designed to quantify Human Fibrinogen with a sensitivity of 0.67 ng/mL.

- Colorimetric sandwich ELISA - 450 nm readout - works on any plate reader
- Validated on a number of sample types including cerebrospinal fluid (CSF)
- Wide dynamic range - quantifies 1.25 - 80 ng/mL

View Alternative Names

Fibrinogen alpha chain, FGA

1 Images
Sandwich ELISA - Human Fibrinogen ELISA Kit (AB108841)
  • sELISA

Unknown

Sandwich ELISA - Human Fibrinogen ELISA Kit (AB108841)

Representative Standard Curve using ab108841.

Key facts

Detection method

Colorimetric

Sample types

Cerebral Spinal Fluid, Saliva, Urine, Milk

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 0.67 ng/mL

Range

1.25 - 80 ng/mL

Assay time

4h

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human Fibrinogen ELISA Kit ab108841 is a sandwich ELISA to measure Human Fibrinogen in cerebral spinal fluid (CSF), saliva, urine, milk with a sensitivity of 0.67 ng/ml.

How the assay works

A Fibrinogen specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Fibrinogen specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Fibrinogen captured in plate.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.

Assay Specificity

Our ELISA kits are rigorously validated to ensure the highest level of consistency and reproducibility. Please check the protocol booklet for more details

Human Fibrinogen ELISA Kit ab108841 protocol summary

1. Add standard or sample to appropriate wells. Incubate at room temperature
2. Wash and add prepared biotin antibody to each well. Incubate at room temperature.
3. Wash and add prepared Streptavidin-Peroxidase Conjugate. Incubate at room temperature
4. Add Chromogen Substrate to each well. Incubate at room temperature
5. Add Stop Solution to each well. Read at 450 nm immediately.

Get results in 90 minutes with our SimpleStep ELISA® kit

Human Fibrinogen ELISA Kit (ab208036)
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Precision

[ { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.9" }, { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "10.3" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab108841-1x96Tests", "assetComponentDetails": [ { "size":"1 x 1 Vial", "name":"Fibrinogen Standard", "number":"AB108841-CMP05", "productcode":"" }, { "size":"2 x 30 mL", "name":"20X Wash Buffer Concentrate", "number":"AB108841-CMP06", "productcode":"" }, { "size":"1 x 11 mL", "name":"Stop Solution", "number":"AB108841-CMP08", "productcode":"" }, { "size":"1 x 7 mL", "name":"Chromogen Substrate", "number":"AB108841-CMP03", "productcode":"" }, { "size":"1 x 30 mL", "name":"10X Diluent N Concentrate", "number":"AB108841-CMP02", "productcode":"" }, { "size":"1 x 120 µL", "name":"50X Biotinylated Human Fibrinogen Antibody", "number":"AB108841-CMP01", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Fibrinogen Microplate (12 x 8 well strips)", "number":"AB108841-CMP04", "productcode":"" }, { "size":"3 x 1 Unit", "name":"Sealing Tapes", "number":"AB108841-CMP07", "productcode":"" }, { "size":"1 x 80 µL", "name":"100X Streptavidin-Peroxidase Conjugate", "number":"AB108841-CMP09", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
Multi
Storage information
Please refer to protocols
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Cleaved by the protease thrombin to yield monomers which, together with fibrinogen beta (FGB) and fibrinogen gamma (FGG), polymerize to form an insoluble fibrin matrix. Fibrin has a major function in hemostasis as one of the primary components of blood clots. In addition, functions during the early stages of wound repair to stabilize the lesion and guide cell migration during re-epithelialization. Was originally thought to be essential for platelet aggregation, based on in vitro studies using anticoagulated blood. However, subsequent studies have shown that it is not absolutely required for thrombus formation in vivo. Enhances expression of SELP in activated platelets via an ITGB3-dependent pathway. Maternal fibrinogen is essential for successful pregnancy. Fibrin deposition is also associated with infection, where it protects against IFNG-mediated hemorrhage. May also facilitate the immune response via both innate and T-cell mediated pathways.
See full target information FGA
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Publications (9)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 14:6380 PubMed37821447

2023

SARS-CoV-2 infection of human lung epithelial cells induces TMPRSS-mediated acute fibrin deposition.

Applications

Unspecified application

Species

Unspecified reactive species

Rachel Erickson,Chang Huang,Cameron Allen,Joanna Ireland,Gwynne Roth,Zhongcheng Zou,Jinghua Lu,Bernard A P Lafont,Nicole L Garza,Beniah Brumbaugh,Ming Zhao,Motoshi Suzuki,Lisa Olano,Joseph Brzostowski,Elizabeth R Fischer,Homer L Twigg,Reed F Johnson,Peter D Sun

American journal of physiology. Lung cellular and molecular physiology 322:L593-L606 PubMed35200041

2022

Fibrinogen mediates cadmium-induced macrophage activation and serves as a predictor of cadmium exposure in chronic obstructive pulmonary disease.

Applications

Unspecified application

Species

Unspecified reactive species

Fu Jun Li,Ranu Surolia,Pooja Singh,Kevin G Dsouza,Crystal T Stephens,Zheng Wang,Rui-Ming Liu,Sejong Bae,Young-Il Kim,Mohammad Athar,Mark T Dransfield,Veena B Antony

Scientific reports 11:4087 PubMed33602999

2021

Cerebrospinal fluid proteome shows disrupted neuronal development in multiple sclerosis.

Applications

Unspecified application

Species

Unspecified reactive species

Ellen F Mosleth,Christian Alexander Vedeler,Kristian Hovde Liland,Anette McLeod,Gerd Haga Bringeland,Liesbeth Kroondijk,Frode Steingrimsen Berven,Artem Lysenko,Christopher J Rawlings,Karim El-Hajj Eid,Jill Anette Opsahl,Bjørn Tore Gjertsen,Kjell-Morten Myhr,Sonia Gavasso

International journal of molecular sciences 22: PubMed33530649

2021

Toward Biofabrication of Resorbable Implants Consisting of a Calcium Phosphate Cement and Fibrin-A Characterization In Vitro and In Vivo.

Applications

Unspecified application

Species

Unspecified reactive species

Tilman Ahlfeld,Anja Lode,Richard Frank Richter,Winnie Pradel,Adrian Franke,Martina Rauner,Bernd Stadlinger,Günter Lauer,Michael Gelinsky,Paula Korn

Molecular & cellular proteomics : MCP 18:1796-1806 PubMed31253657

2019

Identification of Salivary Biomarkers for Oral Cancer Detection with Untargeted and Targeted Quantitative Proteomics Approaches.

Applications

Unspecified application

Species

Unspecified reactive species

Hao-Wei Chu,Kai-Ping Chang,Chia-Wei Hsu,Ian Yi-Feng Chang,Hao-Ping Liu,Yi-Ting Chen,Chih-Ching Wu

Transplantation 103:1821-1833 PubMed30964836

2019

Brain Death Enhances Activation of the Innate Immune System and Leads to Reduced Renal Metabolic Gene Expression.

Applications

Unspecified application

Species

Unspecified reactive species

Laura J Zitur,Peter J Chlebeck,Scott K Odorico,Juan S Danobeitia,Tiffany J Zens,Cees Van Kooten,Michael Eerhart,Jose A Reyes,Megan L Springer,Jennifer M Coonen,Kevin G Brunner,Saverio V Capuano,Anthony M D'Alessandro,Luis A Fernandez

Medical science monitor : international medical jo 24:4146-4153 PubMed29909421

2018

Application of Isobaric Tags for Relative and Absolute Quantification (iTRAQ) Coupled with Two-Dimensional Liquid Chromatography/Tandem Mass Spectrometry in Quantitative Proteomic Analysis for Discovery of Serum Biomarkers for Idiopathic Pulmonary Fibrosis.

Applications

Unspecified application

Species

Unspecified reactive species

Ying Zhang,Qian Xin,Zhen Wu,Chaochao Wang,Yongbin Wang,Qian Wu,Rui Niu

Clinical journal of the American Society of Nephro 12:1922-1929 PubMed28903970

2017

Urinary Fibrinogen as a Predictor of Progression of CKD.

Applications

Unspecified application

Species

Unspecified reactive species

Hongtian Wang,Chunxia Zheng,Yinghui Lu,Qi Jiang,Ru Yin,Ping Zhu,Minlin Zhou,Zhihong Liu

Scientific reports 5:11412 PubMed26081315

2015

Increased cerebrospinal fluid fibrinogen in major depressive disorder.

Applications

Unspecified application

Species

Unspecified reactive species

Kotaro Hattori,Miho Ota,Daimei Sasayama,Sumiko Yoshida,Ryo Matsumura,Tomoko Miyakawa,Yuuki Yokota,Shinobu Yamaguchi,Takamasa Noda,Toshiya Teraishi,Hiroaki Hori,Teruhiko Higuchi,Shinichi Kohsaka,Yu-ichi Goto,Hiroshi Kunugi
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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