AB108842

Human Fibrinogen ELISA Kit

Name: Human Fibrinogen ELISA Kit (ab108842) | Abcam
Brand: Abcam Limited
SKU: AB108842

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(10 Publications)

Human Fibrinogen ELISA Kit is a competitive ELISA designed to quantify Human Fibrinogen with a sensitivity of 0.35 µg/mL

- Colorimetric competitive ELISA - 450 nm readout - works on any plate reader
- Wide dynamic range - quantifies 0.494 - 20 µg/ml
- Cited in over 10 publications

View Alternative Names

Fibrinogen alpha chain, FGA

2 Images

Competitive ELISA - Human Fibrinogen ELISA Kit (AB108842)

cELISA

Unknown

Competitive ELISA - Human Fibrinogen ELISA Kit (AB108842)

Representative Standard Curve using ab108842.

Sandwich ELISA - Human Fibrinogen ELISA Kit (AB108842)

sELISA

Lab

Sandwich ELISA - Human Fibrinogen ELISA Kit (AB108842)

Fibrinogen measured in various samples showing quantity (micrograms) per mL of tested sample

Key facts

Detection method

Colorimetric

Sample types

Plasma

Reacts with

Human

Assay type

Competitive

Sensitivity

= 0.35 µg/mL

Range

0.494 - 20 µg/mL

Assay time

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "cELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Human Fibrinogen in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Fibrinogen levels in plasma.

A Fibrinogen specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently biotinylated Fibrinogen is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is inversely proportional to the amount of Fibrinogen captured in plate.

Get better reproducibility in only 90 minutes with Human Fibrinogen ELISA Kit (ab208036) from our SimpleStep ELISA^® range.

The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "10.4" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.9" } ]

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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab108842-1x96Tests", "assetComponentDetails": [ { "size":"1 x 30 mL", "name":"20X Wash Buffer Concentrate", "number":"AB108842-CMP06", "productcode":"" }, { "size":"1 x 80 µL", "name":"100X Streptavidin-Peroxidase Conjugate", "number":"AB108842-CMP09", "productcode":"" }, { "size":"1 x 1 Vial", "name":"2X Biotinylated Human Fibrinogen (Lyophilized)", "number":"AB108842-CMP01", "productcode":"" }, { "size":"1 x 11 mL", "name":"Stop Solution", "number":"AB108842-CMP08", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Fibrinogen Microplate (12 x 8 well strips)", "number":"AB108842-CMP04", "productcode":"" }, { "size":"1 x 7 mL", "name":"Chromogen Substrate", "number":"AB108842-CMP03", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Fibrinogen Standard", "number":"AB108842-CMP05", "productcode":"" }, { "size":"3 x 1 Unit", "name":"Sealing Tapes", "number":"AB108842-CMP07", "productcode":"" }, { "size":"1 x 30 mL", "name":"10X Diluent N Concentrate", "number":"AB108842-CMP02", "productcode":"" } ] } } }

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

Multi

Storage information

Please refer to protocols

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Product protocols

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Target data

Cleaved by the protease thrombin to yield monomers which, together with fibrinogen beta (FGB) and fibrinogen gamma (FGG), polymerize to form an insoluble fibrin matrix. Fibrin has a major function in hemostasis as one of the primary components of blood clots. In addition, functions during the early stages of wound repair to stabilize the lesion and guide cell migration during re-epithelialization. Was originally thought to be essential for platelet aggregation, based on in vitro studies using anticoagulated blood. However, subsequent studies have shown that it is not absolutely required for thrombus formation in vivo. Enhances expression of SELP in activated platelets via an ITGB3-dependent pathway. Maternal fibrinogen is essential for successful pregnancy. Fibrin deposition is also associated with infection, where it protects against IFNG-mediated hemorrhage. May also facilitate the immune response via both innate and T-cell mediated pathways.

See full target information FGA

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Publications (10)

Recent publications for all applications. Explore the full list and refine your search

Animals : an open access journal from MDPI 13: PubMed36899749

2023

Immunological and Oxidative Biomarkers in Bovine Serum from Healthy, Clinical, and Sub-Clinical Mastitis Caused by and Infection.

Applications

Unspecified application

Species

Unspecified reactive species

Asmaa Sadat,Alshimaa M M Farag,Driss Elhanafi,Amal Awad,Ehab Kotb Elmahallawy,Noorah Alsowayeh,Manal F El-Khadragy,Gehad E Elshopakey

European journal of nutrition 62:1231-1238 PubMed36495341

2022

Dietary fat intake is associated with insulin resistance and an adverse vascular profile in patients with T1D: a pooled analysis.

Applications

Unspecified application

Species

Unspecified reactive species

Noppadol Kietsiriroje,Hanya Shah,Marios Zare,Lauren L O'Mahoney,Daniel J West,Sam M Pearson,Ramzi A Ajjan,Matthew D Campbell

Diabetes & vascular disease research 19:14791641221103217 PubMed35657731

2022

Glucose variability is associated with an adverse vascular profile but only in the presence of insulin resistance in individuals with type 1 diabetes: An observational study.

Applications

Unspecified application

Species

Unspecified reactive species

Noppadol Kietsiriroje,Sam M Pearson,Lauren L O'Mahoney,Daniel J West,Robert As Ariëns,Ramzi A Ajjan,Matthew D Campbell

International journal of molecular sciences 22: PubMed34445472

2021

Apheresis Platelet Rich-Plasma for Regenerative Medicine: An In Vitro Study on Osteogenic Potential.

Applications

Unspecified application

Species

Unspecified reactive species

Stefano Pulcini,Lucia Merolle,Chiara Marraccini,Eleonora Quartieri,Daniele Mori,Davide Schiroli,Pamela Berni,Barbara Iotti,Erminia Di Bartolomeo,Roberto Baricchi,Roberto Sala,Thelma A Pertinhez

Journal of endocrinological investigation 44:2417-2426 PubMed33730349

2021

Estimated glucose disposal rate as a candidate biomarker for thrombotic biomarkers in T1D: a pooled analysis.

Applications

Unspecified application

Species

Unspecified reactive species

L L O'Mahoney,N Kietsiriroje,S Pearson,D J West,M Holmes,R A Ajjan,M D Campbell

Oncotarget 9:14862-14881 PubMed29599912

2018

Coagulation cascade and complement system in systemic lupus erythematosus.

Applications

Unspecified application

Species

Unspecified reactive species

Yan Liang,Shang-Bo Xie,Chang-Hao Wu,Yuan Hu,Qin Zhang,Si Li,Yin-Guang Fan,Rui-Xue Leng,Hai-Feng Pan,Hua-Bao Xiong,Dong-Qing Ye

Environmental research 158:508-521 PubMed28709033

2017

Vegetated land cover near residence is associated with reduced allostatic load and improved biomarkers of neuroendocrine, metabolic and immune functions.

Applications

Unspecified application

Species

Unspecified reactive species

Andrey I Egorov,Shannon M Griffin,Reagan R Converse,Jennifer N Styles,Elizabeth A Sams,Anthony Wilson,Laura E Jackson,Timothy J Wade

Diabetes & vascular disease research 14:336-344 PubMed28322071

2017

An additional bolus of rapid-acting insulin to normalise postprandial cardiovascular risk factors following a high-carbohydrate high-fat meal in patients with type 1 diabetes: A randomised controlled trial.

Applications

Unspecified application

Species

Unspecified reactive species

Matthew D Campbell,Mark Walker,Ramzi A Ajjan,Karen M Birch,Javier T Gonzalez,Daniel J West

European journal of clinical investigation 46:1031-1040 PubMed27727459

2016

Sex-specific vitamin D effects on blood coagulation among overweight adults.

Applications

Unspecified application

Species

Unspecified reactive species

Nasser M Al-Daghri,Majed S Alokail,Antigoni Manousopoulou,Ashley Heinson,Omar Al-Attas,Yousef Al-Saleh,Shaun Sabico,Sobhy Yakout,Christopher H Woelk,George P Chrousos,Spiros D Garbis

International journal of nanomedicine 10 Spec Iss:155-63 PubMed26347331

2015

A regenerative label-free fiber optic sensor using surface plasmon resonance for clinical diagnosis of fibrinogen.

Applications

Unspecified application

Species

Unspecified reactive species

Tan Tai Nguyen,Sun Oh Bea,Dong Min Kim,Won Jung Yoon,Jin-Won Park,Seong Soo A An,Heongkyu Ju

View all publications

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websiteProtocolBooklet

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Human Fibrinogen ELISA Kit

Competitive ELISA - Human Fibrinogen ELISA Kit (AB108842)

Sandwich ELISA - Human Fibrinogen ELISA Kit (AB108842)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay time

Assay Platform

Reactivity data

Product details

Precision

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Product protocols

Target data

Publications (10)

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