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AB325257

Human Fibrinogen ELISA Kit

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Human Fibrinogen ELISA Kit is a competitive ELISA designed to quantify Human Fibrinogen with a sensitivity of 2.9 µg/mL

- Colorimetric competitive ELISA - 450 nm readout - works on any plate reader
- Wide dynamic range - quantifies 5 - 80 µg/ml
- Cited in over 10 publications

View Alternative Names

Fibrinogen alpha chain, FGA

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Competitive ELISA - Human Fibrinogen ELISA Kit (AB325257)
  • cELISA

Supplier Data

Competitive ELISA - Human Fibrinogen ELISA Kit (AB325257)

Example of Fibrinogen standard curve. The standard curve was prepared as described in Section 10 of the protocol booklet. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Plasma

Reacts with

Human

Assay type

Competitive

Results type

Quantitative

Sensitivity

= 2.9 µg/mL

Range

5 - 80 µg/mL

Assay time

3h

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "cELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human Fibrinogen ELISA Kit is designed for the quantitative measurement of Fibrinogen levels in plasma.

A Fibrinogen specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently biotinylated Fibrinogen is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is inversely proportional to the amount of Fibrinogen captured in plate.

Get better reproducibility in only 90 minutes with Human Fibrinogen ELISA Kit (ab208036) from our SimpleStep ELISA® range.

The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 20, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 10.8" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 20, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 4.7" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
Multi
Storage information
Please refer to protocols
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Product protocols

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Target data

Cleaved by the protease thrombin to yield monomers which, together with fibrinogen beta (FGB) and fibrinogen gamma (FGG), polymerize to form an insoluble fibrin matrix. Fibrin has a major function in hemostasis as one of the primary components of blood clots. In addition, functions during the early stages of wound repair to stabilize the lesion and guide cell migration during re-epithelialization. Was originally thought to be essential for platelet aggregation, based on in vitro studies using anticoagulated blood. However, subsequent studies have shown that it is not absolutely required for thrombus formation in vivo. Enhances expression of SELP in activated platelets via an ITGB3-dependent pathway. Maternal fibrinogen is essential for successful pregnancy. Fibrin deposition is also associated with infection, where it protects against IFNG-mediated hemorrhage. May also facilitate the immune response via both innate and T-cell mediated pathways.
See full target information FGA
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websiteProtocolBooklet
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Product promise

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