AB326383

Human Fibrinogen ELISA Kit, Chemiluminescent

SimpleStep Ignite™ ELISA
Recombinant
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Human Fibrinogen ELISA Kit, Chemiluminescent is a 55-min single-wash SimpleStep Ignite™ ELISA for the rapid quantification of Human Fibrinogen with higher sensitivity and broader measuring range.

- Single 30 min incubation and single-wash step for results in less than an hour.
- Chemiluminescent detection allows a broad dynamic range: 82.3 - 60000 pg/ml with a sensitivity of 40.37pg/mL.
- Design your own immunoassay: we also offer the conjugation-ready antibody pair.
-Patent pending

View Alternative Names

Fibrinogen alpha chain, FGA

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Sandwich ELISA - Human Fibrinogen ELISA Kit, Chemiluminescent (AB326383)

sELISA

Lab

Sandwich ELISA - Human Fibrinogen ELISA Kit, Chemiluminescent (AB326383)

SimpleStep ELISA® Ignite technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 55 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

sELISA

Lab

Sandwich ELISA - Human Fibrinogen ELISA Kit, Chemiluminescent (AB326383)

Standard curve comparison between the Chemiluminescent Human Fibrinogen SimpleStep ELISA and colorimetric Human Fibrinogen SimpleStep ELISA (ab241383) in Sample Diluent NS. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Lab

Sandwich ELISA - Human Fibrinogen ELISA Kit, Chemiluminescent (AB326383)

Dynamic range comparison between the Chemiluminescent Human Fibrinogen SimpleStep ELISA, colorimetric Human Fibrinogen SimpleStep ELISA (ab241383), and leading chemiluminescent competitor. The Chemiluminescent SimpleStep ELISA kit shows increased sensitivity and increased dynamic range.

sELISA

Supplier Data

Sandwich ELISA - Human Fibrinogen ELISA Kit, Chemiluminescent (AB326383)

Percent reactivity against related fibrinogen proteins.

The following recombinant/native proteins were prepared at 50 ng/mL and assayed for cross-reactivity. Percent reactivity is determined by interpolating the protein concentrations from the standard curve and expressing the interpolated concentrations as a percentage of the protein concentration assayed at the same concentration.

sELISA

Supplier Data

Sandwich ELISA - Human Fibrinogen ELISA Kit, Chemiluminescent (AB326383)

Serum from ten individual healthy human female donors was measured in duplicate.

Interpolated dilution factor corrected values are plotted (mean +/- SD n=2). The mean Fibrinogen concentration was determined to be 1070 ng/mL with a range of 647 – 2027 ng/mL.

sELISA

Supplier Data

Sandwich ELISA - Human Fibrinogen ELISA Kit, Chemiluminescent (AB326383)

Interpolated concentrations of native Fibrinogen in human serum and plasma samples.

The concentrations of Fibrinogen were measured in duplicates interpolated from the Fibrinogen standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 1 : 670 plasma (citrate) 1 : 5x10⁵ plasma (EDTA) 1 : 5x10⁵ and plasma (heparin) 1 : 5x10⁵. The interpolated dilution factor corrected values are plotted (mean +/- SD n=2). The mean Fibrinogen concentration was determined to be 3.45 µg/mL in serum 2.86 mg/mL in plasma (citrate) 2.96 mg/mL in plasma (EDTA) and 2.64 mg/mL in plasma (heparin).

sELISA

Supplier Data

Sandwich ELISA - Human Fibrinogen ELISA Kit, Chemiluminescent (AB326383)

Interpolated concentrations of native Fibrinogen in human urine saliva milk and cell culture supernatant samples.

The concentrations of Fibrinogen were measured in duplicates interpolated from the Fibrinogen standard curves and corrected for sample dilution. Undiluted samples are as follows : urine 1 : 1.7 saliva 1 : 133 milk 1 : 133 and HepG2 supernatant 1 : 500. The interpolated dilution factor corrected values are plotted (mean +/- SD n=2). The mean Fibrinogen concentration was determined to be 5.93 ng/mL in urine 934.5 ng/mL in saliva 199.7 ng/mL in milk and 1.35 µg/mL in HepG2 supernatant.

sELISA

Supplier Data

Sandwich ELISA - Human Fibrinogen ELISA Kit, Chemiluminescent (AB326383)

Linearity of dilution is determined based on interpolated values from the standard curve. Linearity of dilution defines a sample concentration interval in which interpolated target concentrations are directly proportional to sample dilution.

Native Fibrinogen was measured in the following biological samples in a 2-fold dilution series. Sample dilutions are made in Sample Diluent NS.

sELISA

Supplier Data

Sandwich ELISA - Human Fibrinogen ELISA Kit, Chemiluminescent (AB326383)

Native Fibrinogen was measured in the following biological samples in a 2-fold dilution series. Sample dilutions are made in Sample Diluent NS.

Key facts

Detection method

Luminescent

Sample types

Cell culture supernatant, Milk, Serum, EDTA Plasma, Urine, Saliva, Heparin Plasma, Citrate plasma

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 40.37 pg/mL

Range

82.3 - 60000 pg/mL

Assay time

55m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Human Fibrinogen ELISA Kit, Chemiluminescent (ab326383) is a rapid single-wash sandwich 55-min ELISA to measure Human Fibrinogen in Cell culture supernatant, Milk, Serum, EDTA Plasma, Urine, Saliva, Heparin Plasma, Citrate plasma with a sensitivity of 40.37 pg/mL.

How the assay works

Human Fibrinogen ELISA Kit, Chemiluminescent (ab326383) SimpleStep Ignite™ ELISA employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA^® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step during a 30 min only incubation, significantly reducing assay time. By utilizing a chemiluminscent detection method this improves the sensitivity and dynamic range when compared to traditional colorimetric ELISAs.

Assay Specificity

Our SimpleStep Ignite™ ELISA kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human Fibrinogen (ab326383) protocol summary

1. Mix : add samples / standards to the wells together with the capture and detector antibody cocktail. Incubate 30 min at room temperature
2. Wash
3. Add ChemiHRP development solution - incubate for 2 min
4. Read the results on a plate reader capable of luminescence detection

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-Fibrinogen antibody [EPR19765-189] - BSA and Azide free (Capture) ab244636
- Anti-Fibrinogen antibody [EPR19765-63] - BSA and Azide free (Detector) ab244897

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Precision

[ { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 3.4" }, { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 11.7" } ]

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Recovery

[ { "sample": "Citrate plasma", "range": "95 - 95 %", "average": "= 95" }, { "sample": "Cell culture supernatant", "range": "93 - 95 %", "average": "= 94" }, { "sample": "Milk", "range": "102 - 105 %", "average": "= 103" }, { "sample": "Serum", "range": "90 - 94 %", "average": "= 91" }, { "sample": "EDTA Plasma", "range": "91 - 94 %", "average": "= 93" }, { "sample": "Urine", "range": "99 - 100 %", "average": "= 100" }, { "sample": "Saliva", "range": "98 - 104 %", "average": "= 101" }, { "sample": "Heparin Plasma", "range": "91 - 94 %", "average": "= 96" } ]

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What's included?

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Product protocols

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Target data

Cleaved by the protease thrombin to yield monomers which, together with fibrinogen beta (FGB) and fibrinogen gamma (FGG), polymerize to form an insoluble fibrin matrix. Fibrin has a major function in hemostasis as one of the primary components of blood clots. In addition, functions during the early stages of wound repair to stabilize the lesion and guide cell migration during re-epithelialization. Was originally thought to be essential for platelet aggregation, based on in vitro studies using anticoagulated blood. However, subsequent studies have shown that it is not absolutely required for thrombus formation in vivo. Enhances expression of SELP in activated platelets via an ITGB3-dependent pathway. Maternal fibrinogen is essential for successful pregnancy. Fibrin deposition is also associated with infection, where it protects against IFNG-mediated hemorrhage. May also facilitate the immune response via both innate and T-cell mediated pathways.

See full target information FGA

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websiteProtocolBooklet

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Product promise

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