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AB229419

Human Fibrinogen ELISA Kit, Fluorescent

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Human Fibrinogen ELISA Kit, Fluorescent is a single-wash 90-min Simplestep used to quantify Human Fibrinogen with a sensitivity of 29 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Fluorescent Sandwich ELISA - 530/570/590 nm readout : works on any standard plate reader

View Alternative Names

Fibrinogen alpha chain, FGA

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ELISA - Human Fibrinogen ELISA Kit, Fluorescent (AB229419)
  • ELISA

Supplier Data

ELISA - Human Fibrinogen ELISA Kit, Fluorescent (AB229419)
Sandwich ELISA - Human Fibrinogen ELISA Kit, Fluorescent (AB229419)
  • sELISA

Supplier Data

Sandwich ELISA - Human Fibrinogen ELISA Kit, Fluorescent (AB229419)

Example of human Fibrinogen standard curve in Sample Diluent NS.

The Fibrinogen standard curve was prepared as described in Section 10. Raw data generated on SpectraMax M4 Multi-Mode Microplate Reader is shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human Fibrinogen ELISA Kit, Fluorescent (AB229419)
  • sELISA

Supplier Data

Sandwich ELISA - Human Fibrinogen ELISA Kit, Fluorescent (AB229419)

Interpolated concentrations of native Fibrinogen in human serum and plasma samples.

The concentrations of Fibrinogen were measured in duplicates, interpolated from the Fibrinogen standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 1 : 670, plasma (citrate) 1 : 5x105, plasma (EDTA) 1 : 5x105, and plasma (heparin) 1 : 5x105. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Fibrinogen concentration was determined to be 3.45 μg/mL in serum, 2.86 mg/mL in plasma (citrate), 2.96 mg/mL in plasma (EDTA), and 2.64 mg/mL in plasma (heparin).

Sandwich ELISA - Human Fibrinogen ELISA Kit, Fluorescent (AB229419)
  • sELISA

Supplier Data

Sandwich ELISA - Human Fibrinogen ELISA Kit, Fluorescent (AB229419)

Serum from ten individual healthy human female donors was measured in duplicate.

Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Fibrinogen concentration was determined to be 1,070 ng/mL with a range of 647 – 2,027 ng/mL.

Sandwich ELISA - Human Fibrinogen ELISA Kit, Fluorescent (AB229419)
  • sELISA

Supplier Data

Sandwich ELISA - Human Fibrinogen ELISA Kit, Fluorescent (AB229419)

Interpolated concentrations of native Fibrinogen in human urine, saliva, milk and cell culture supernatant samples.

The concentrations of Fibrinogen were measured in duplicates, interpolated from the Fibrinogen standard curves and corrected for sample dilution. Undiluted samples are as follows : urine 1 : 1.7, saliva 1 : 133, milk 1 : 133 and HepG2 supernatant 1 : 500. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Fibrinogen concentration was determined to be 5.93 ng/mL in urine, 934.5 ng/mL in saliva, 199.7 ng/mL in milk and 1.35 μg/mL in HepG2 supernatant.

Sandwich ELISA - Human Fibrinogen ELISA Kit, Fluorescent (AB229419)
  • sELISA

Supplier Data

Sandwich ELISA - Human Fibrinogen ELISA Kit, Fluorescent (AB229419)

Percent reactivity against related fibrinogen proteins.

The following recombinant/native proteins were prepared at 50 ng/mL and assayed for cross-reactivity. Percent reactivity is determined by interpolating the protein concentrations from the standard curve and expressing the interpolated concentrations as a percentage of the protein concentration assayed at the same concentration.

Key facts

Detection method

Fluorescent

Sample types

Saliva, Urine, Heparin Plasma, Citrate plasma, Cell culture supernatant, Milk, Serum, EDTA Plasma

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 29 pg/mL

Range

15.63 - 64000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human Fibrinogen ELISA Kit, Fluorescent ab229419 is a rapid single-wash 90-min sandwich ELISA to measure Human Fibrinogen in serum, heparin plasma, citrate plasma, EDTA plasma, cell culture supernatant, saliva, urine. This CatchPoint SimpleStep sensitivity is 29 pg/mL.

How the assay works

Human Fibrinogen CatchPoint SimpleStep ELISA employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. CatchPoint HRP Development Solution containing the Stoplight Red Substrate is added. During incubation, the substrate is catalyzed by HRP generating a fluorescent product. Signal is generated proportionally to the amount of bound analyte and the intensity is measured in a fluorescence plater reader at 530/570/590 nm Excitation/Cutoff/Emission. This kit is optimised for Molecular Devices Readers: If using a Molecular Devices' plate reader supported by SoftMax® Pro software, a preconfigured protocol for these CatchPoint SimpleStep ELISA Kits is available with all the protocol and analysis settings at www.softmaxpro.org.

Assay Specificity

Our SimpleStep ELISA® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human Fibrinogen ELISA Kit, Fluorescent ab229419 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a fluorescent plate reader at 530/570/590 nm Excitation/Cutoff/Emission.

Colorimetric version

This kit is also available with a colorimetric readout as Human Fibrinogen ELISA Kit - high sensitivity ab241383.
Both the fluroescent and colorimetric version share the same antibody pair and protein standard.

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation

- Anti-Fibrinogen antibody [EPR19765-189] - BSA and Azide free (Capture) ab244636
- Anti-Fibrinogen antibody [EPR19765-63] - BSA and Azide free (Detector) ab244897
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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "11.7" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.4" } ]
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Recovery

[ { "sample": "Cell culture supernatant", "range": "93 - 95 %", "average": "= 94" }, { "sample": "Citrate plasma", "range": "91 - 96 %", "average": "= 94" }, { "sample": "EDTA Plasma", "range": "91 - 94 %", "average": "= 93" }, { "sample": "Heparin Plasma", "range": null, "average": "= 95" }, { "sample": "Milk", "range": "102 - 105 %", "average": "= 103" }, { "sample": "Saliva", "range": "98 - 104 %", "average": "= 101" }, { "sample": "Serum", "range": "90 - 94 %", "average": "= 91" }, { "sample": "Urine", "range": "99 - 100 %", "average": "= 100" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

Cleaved by the protease thrombin to yield monomers which, together with fibrinogen beta (FGB) and fibrinogen gamma (FGG), polymerize to form an insoluble fibrin matrix. Fibrin has a major function in hemostasis as one of the primary components of blood clots. In addition, functions during the early stages of wound repair to stabilize the lesion and guide cell migration during re-epithelialization. Was originally thought to be essential for platelet aggregation, based on in vitro studies using anticoagulated blood. However, subsequent studies have shown that it is not absolutely required for thrombus formation in vivo. Enhances expression of SELP in activated platelets via an ITGB3-dependent pathway. Maternal fibrinogen is essential for successful pregnancy. Fibrin deposition is also associated with infection, where it protects against IFNG-mediated hemorrhage. May also facilitate the immune response via both innate and T-cell mediated pathways.
See full target information FGA
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websiteProtocolBooklet
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