Human FGA ELISA kit, 90-min SimpleStep ELISA® (ab241383)

Human Fibrinogen (FGA) ELISA kit is a single-wash, 90-minute SimpleStep ELISA® used to quantify fibrinogen levels with a sensitivity of 29 pg/mL. The assay employs a simple Mix-Wash-Read protocol with just one incubation and wash step.

- Colorimetric assay – 450 nm readout, compatible with any standard plate reader
- Different formats for different needs – 10 x 96 well plates for bulk orders andn 384-well format available for high-throughput applications
- Design your own immunoassay: we also offer the conjugation-ready antibody pair in a carrier-free format

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Key facts

Detection method: Colorimetric
Sample types: Saliva, Urine, Heparin Plasma, Citrate plasma, Cell culture supernatant, Milk, Serum, EDTA Plasma
Assay type: Sandwich (quantitative)
Reactive species: Human
Range: 125 - 8000 pg/mL
Assay time: 1h 30m
Sensitivity: = 29 pg/mL

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Reactivity data

Application	Reactivity	Dilution info	Notes
Application sELISA	Reactivity Reacts	Dilution info -	Notes -

Target data

See full target information FGA

Function

Cleaved by the protease thrombin to yield monomers which, together with fibrinogen beta (FGB) and fibrinogen gamma (FGG), polymerize to form an insoluble fibrin matrix. Fibrin has a major function in hemostasis as one of the primary components of blood clots. In addition, functions during the early stages of wound repair to stabilize the lesion and guide cell migration during re-epithelialization. Was originally thought to be essential for platelet aggregation, based on in vitro studies using anticoagulated blood. However, subsequent studies have shown that it is not absolutely required for thrombus formation in vivo. Enhances expression of SELP in activated platelets via an ITGB3-dependent pathway. Maternal fibrinogen is essential for successful pregnancy. Fibrin deposition is also associated with infection, where it protects against IFNG-mediated hemorrhage. May also facilitate the immune response via both innate and T-cell mediated pathways.

Alternative names

Fibrinogen alpha chain, FGA

What's included?

1 x 384 Tests

Components

10X Human Fibrinogen Capture Antibody

1 x 600 µL

10X Human Fibrinogen Detector Antibody

1 x 600 µL

10X Wash Buffer PT (ab206977)

1 x 20 mL

384 well CaptSure™ microplates

1 x 1 Unit

Antibody Diluent 4BI

1 x 6 mL

Human Fibrinogen Lyophilized Purified Protein

2 x 1 Vial

Plate Seals

1 x 1 Unit

Sample Diluent NS (ab193972)

1 x 250 mL

Stop Solution

2 x 12 mL

TMB Development Solution

2 x 12 mL

Key facts

Detection method: Colorimetric
Sample types: Saliva, Urine, Heparin Plasma, Citrate plasma, Cell culture supernatant, Milk, Serum, EDTA Plasma
Assay type: Sandwich (quantitative)
Reactive species: Human
Range: 125 - 8000 pg/mL
Assay time: 1h 30m
Assay Platform: Pre-coated microplate (12 x 8 well strips)
Sensitivity: = 29 pg/mL

Precision

Intra assay

Sample	n	mean	SD	C.V.
Sample Serum	n 8	mean -	SD -	C.V. 3.4

Inter assay

Sample	n	mean	SD	C.V.
Sample Serum	n 3	mean -	SD -	C.V. 11.7

Recovery

Sample specific recovery

Sample type	Average %	Range
Sample type Cell culture supernatant	Average % = 94	Range 93 - 95 %
Sample type Milk	Average % = 103	Range 102 - 105 %
Sample type Serum	Average % = 91	Range 90 - 94 %
Sample type EDTA Plasma	Average % = 93	Range 91 - 94 %
Sample type Urine	Average % = 100	Range 99 - 100 %
Sample type Saliva	Average % = 101	Range 98 - 104 %
Sample type Heparin Plasma	Average % = 95	Range
Sample type Citrate plasma	Average % = 94	Range 91 - 96 %

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: +4°C
Storage information: +4°C

Notes

Human Fibrinogen ELISA Kit - high sensitivity ab241383 is a rapid single-wash 90-min ELISA kit to measure Human Fibrinogen in saliva, urine, cell culture supernatant, milk, plasma and serum. This SimpleStep ELISA® sensitivity is 29 pg/mL.

How the assay works

Human Fibrinogen SimpleStep ELISA® employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Assay Specificity

This kit recognizes both native and recombinant human Fibrinogen protein in serum, plasma, urine, saliva, milk, and cell culture supernatant only. Cell and tissue extract samples have not been tested with this kit.

Human Fibrinogen ELISA Kit - high sensitivity ab241383 protocol summary:

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate for 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add stop solution
5. Read the results on a plate reader at 450nm

How other researchers are using Human Fibrinogen ELISA Kit - high sensitivity ab241383

Human Fibrinogen ELISA Kit - high sensitivity ab241383 has been used to study fibrinogen function and the effects of a novel mutation on fibinogen synthesis, secretion, and polymerization⁽¹⁾, extracorporeal bioengineered liver grafts for treating acute liver failure⁽²⁾, and CRSPR/Cas9 to upregulate expression of coagulation factors II, VII, IX, X, and fibrinogen⁽³⁾.

References:

⁽¹⁾Fei Chen et al. 2024, PMID: 38233949,
⁽²⁾Jeff J Ross et al. 2023, PMID: 37892931,
⁽³⁾Mark J Osborn et al. 2022, PMID: 35563479

Save your precious samples

Our 384-well Human Fibrinogen ELISA Kit - high sensitivity only requires a maxmum of 12.5µL

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation

- Human Fibrinogen Antibody Pair - BSA and Azide free - high sensitivity Human Fibrinogen Antibody Pair - BSA and Azide free - high sensitivity ab256605
- Anti-Fibrinogen antibody [EPR19765-189] - BSA and Azide free (Capture) Anti-Fibrinogen antibody [EPR19765-189] - BSA and Azide free (Capture) ab244636
- Anti-Fibrinogen antibody [EPR19765-63] - BSA and Azide free (Detector) Anti-Fibrinogen antibody [EPR19765-63] - BSA and Azide free (Detector) ab244897

Related and recommended products

Human Fibrinogen ELISA Kit - high sensitivity ab241383 is commonly used to study fibrinogen levels in coagulation, fibrinolysis, and liver function.

Other ELISA kits to measure coagulation and liver health include:

- Human AST ELISA Kit (Aspartate Aminotransferase) Human AST ELISA Kit (Aspartate Aminotransferase) ab263881
-Human PAI1 ELISA Kit (SERPINE1) Human PAI1 ELISA Kit (SERPINE1) ab269373
- Human Plasminogen ELISA Kit (PLG) Human Plasminogen ELISA Kit (PLG) ab108893

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11 product images

Sandwich ELISA - Human Fibrinogen ELISA Kit - high sensitivity (ab241383)
Example of human Fibrinogen standard curve in Sample Diluent NS.
The Fibrinogen standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human Fibrinogen ELISA Kit - high sensitivity (ab241383)
Interpolated concentrations of native Fibrinogen in human serum and plasma samples.
The concentrations of Fibrinogen were measured in duplicates, interpolated from the Fibrinogen standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 1:670, plasma (citrate) 1:5x10⁵, plasma (EDTA) 1:5x10⁵, and plasma (heparin) 1:5x10⁵. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Fibrinogen concentration was determined to be 3.45 μg/mL in serum, 2.86 mg/mL in plasma (citrate), 2.96 mg/mL in plasma (EDTA), and 2.64 mg/mL in plasma (heparin).
Sandwich ELISA - Human Fibrinogen ELISA Kit - high sensitivity (ab241383)
Interpolated concentrations of native Fibrinogen in human urine, saliva, milk and cell culture supernatant samples.
The concentrations of Fibrinogen were measured in duplicates, interpolated from the Fibrinogen standard curves and corrected for sample dilution. Undiluted samples are as follows: urine 1:1.7, saliva 1:133, milk 1:133 and HepG2 supernatant 1:500. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Fibrinogen concentration was determined to be 5.93 ng/mL in urine, 934.5 ng/mL in saliva, 199.7 ng/mL in milk and 1.35 μg/mL in HepG2 supernatant.
Sandwich ELISA - Human Fibrinogen ELISA Kit - high sensitivity (ab241383)
Human Fibrinogen Standard Curve Comparison.
Standard Curve comparison between human Fibrinogen current (new) SimpleStep ELISA kit and original ELISA kit (ab208036). The current SimpleStep ELISA kit shows increased sensitivity.
Sandwich ELISA - Human Fibrinogen ELISA Kit - high sensitivity (ab241383)
Serum from ten individual healthy human female donors was measured in duplicate.
Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Fibrinogen concentration was determined to be 1,070 ng/mL with a range of 647 – 2,027 ng/mL.
Sandwich ELISA - Human Fibrinogen ELISA Kit - high sensitivity (ab241383)
Example of human Fibrinogen standard curve in Sample Diluent NS.
The Fibrinogen standard curve was prepared as described. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human Fibrinogen ELISA Kit - high sensitivity (ab241383)
Linearity of dilution is determined based on interpolated values from the standard curve. Linearity of dilution defines a sample concentration interval in which interpolated target concentrations are directly proportional to sample dilution.
Native Fibrinogen was measured in the following biological samples in a 2-fold dilution series. Sample dilutions are made in Sample Diluent NS.
Sandwich ELISA - Human Fibrinogen ELISA Kit - high sensitivity (ab241383)
Linearity of dilution is determined based on interpolated values from the standard curve. Linearity of dilution defines a sample concentration interval in which interpolated target concentrations are directly proportional to sample dilution.
Native Fibrinogen was measured in the following biological samples in a 2-fold dilution series. Sample dilutions are made in Sample Diluent NS.
Sandwich ELISA - Human Fibrinogen ELISA Kit - high sensitivity (ab241383)
Example of human Fibrinogen standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human Fibrinogen ELISA Kit - high sensitivity (ab241383)
Interpolated concentration of native Fibrinogen was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 1:670 serum. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.
Sandwich ELISA - Human Fibrinogen ELISA Kit - high sensitivity (ab241383)
Percent reactivity against related fibrinogen proteins.
The following recombinant/native proteins were prepared at 50 ng/mL and assayed for cross-reactivity. Percent reactivity is determined by interpolating the protein concentrations from the standard curve and expressing the interpolated concentrations as a percentage of the protein concentration assayed at the same concentration.