JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB241383

Human Fibrinogen ELISA Kit - high sensitivity

Be the first to review this product! Submit a review

|

(6 Publications)

Human Fibrinogen (FGA) ELISA kit is a single-wash, 90-minute SimpleStep ELISA® used to quantify fibrinogen levels with a sensitivity of 29 pg/mL. The assay employs a simple Mix-Wash-Read protocol with just one incubation and wash step.

- Colorimetric assay – 450 nm readout, compatible with any standard plate reader
- Different formats for different needs – 10 x 96 well plates for bulk orders and 384-well format available for high-throughput applications
- Design your own immunoassay: we also offer the conjugation-ready antibody pair in a carrier-free format

View Alternative Names

Fibrinogen alpha chain, FGA

12 Images
ELISA - Human Fibrinogen ELISA Kit - high sensitivity (AB241383)
  • ELISA

Supplier Data

ELISA - Human Fibrinogen ELISA Kit - high sensitivity (AB241383)
Sandwich ELISA - Human Fibrinogen ELISA Kit - high sensitivity (AB241383)
  • sELISA

Lab

Sandwich ELISA - Human Fibrinogen ELISA Kit - high sensitivity (AB241383)

Interpolated concentration of native Fibrinogen was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 1 : 670 serum. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

Sandwich ELISA - Human Fibrinogen ELISA Kit - high sensitivity (AB241383)
  • sELISA

Lab

Sandwich ELISA - Human Fibrinogen ELISA Kit - high sensitivity (AB241383)

Example of human Fibrinogen standard curve in Sample Diluent NS in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human Fibrinogen ELISA Kit - high sensitivity (AB241383)
  • sELISA

Supplier Data

Sandwich ELISA - Human Fibrinogen ELISA Kit - high sensitivity (AB241383)

Serum from ten individual healthy human female donors was measured in duplicate.

Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Fibrinogen concentration was determined to be 1,070 ng/mL with a range of 647 – 2,027 ng/mL.

Sandwich ELISA - Human Fibrinogen ELISA Kit - high sensitivity (AB241383)
  • sELISA

Supplier Data

Sandwich ELISA - Human Fibrinogen ELISA Kit - high sensitivity (AB241383)

Human Fibrinogen Standard Curve Comparison.

Standard Curve comparison between human Fibrinogen current (new) SimpleStep ELISA kit and original ELISA kit (ab208036). The current SimpleStep ELISA kit shows increased sensitivity.

Sandwich ELISA - Human Fibrinogen ELISA Kit - high sensitivity (AB241383)
  • sELISA

Supplier Data

Sandwich ELISA - Human Fibrinogen ELISA Kit - high sensitivity (AB241383)

Linearity of dilution is determined based on interpolated values from the standard curve. Linearity of dilution defines a sample concentration interval in which interpolated target concentrations are directly proportional to sample dilution.

Native Fibrinogen was measured in the following biological samples in a 2-fold dilution series. Sample dilutions are made in Sample Diluent NS.

Sandwich ELISA - Human Fibrinogen ELISA Kit - high sensitivity (AB241383)
  • sELISA

Supplier Data

Sandwich ELISA - Human Fibrinogen ELISA Kit - high sensitivity (AB241383)

Linearity of dilution is determined based on interpolated values from the standard curve. Linearity of dilution defines a sample concentration interval in which interpolated target concentrations are directly proportional to sample dilution.

Native Fibrinogen was measured in the following biological samples in a 2-fold dilution series. Sample dilutions are made in Sample Diluent NS.

Sandwich ELISA - Human Fibrinogen ELISA Kit - high sensitivity (AB241383)
  • sELISA

Supplier Data

Sandwich ELISA - Human Fibrinogen ELISA Kit - high sensitivity (AB241383)

Example of human Fibrinogen standard curve in Sample Diluent NS.

The Fibrinogen standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human Fibrinogen ELISA Kit - high sensitivity (AB241383)
  • sELISA

Supplier Data

Sandwich ELISA - Human Fibrinogen ELISA Kit - high sensitivity (AB241383)

Example of human Fibrinogen standard curve in Sample Diluent NS.

The Fibrinogen standard curve was prepared as described. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human Fibrinogen ELISA Kit - high sensitivity (AB241383)
  • sELISA

Supplier Data

Sandwich ELISA - Human Fibrinogen ELISA Kit - high sensitivity (AB241383)

Interpolated concentrations of native Fibrinogen in human urine, saliva, milk and cell culture supernatant samples.

The concentrations of Fibrinogen were measured in duplicates, interpolated from the Fibrinogen standard curves and corrected for sample dilution. Undiluted samples are as follows : urine 1 : 1.7, saliva 1 : 133, milk 1 : 133 and HepG2 supernatant 1 : 500. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Fibrinogen concentration was determined to be 5.93 ng/mL in urine, 934.5 ng/mL in saliva, 199.7 ng/mL in milk and 1.35 μg/mL in HepG2 supernatant.

Sandwich ELISA - Human Fibrinogen ELISA Kit - high sensitivity (AB241383)
  • sELISA

Supplier Data

Sandwich ELISA - Human Fibrinogen ELISA Kit - high sensitivity (AB241383)

Percent reactivity against related fibrinogen proteins.

The following recombinant/native proteins were prepared at 50 ng/mL and assayed for cross-reactivity. Percent reactivity is determined by interpolating the protein concentrations from the standard curve and expressing the interpolated concentrations as a percentage of the protein concentration assayed at the same concentration.

Sandwich ELISA - Human Fibrinogen ELISA Kit - high sensitivity (AB241383)
  • sELISA

Supplier Data

Sandwich ELISA - Human Fibrinogen ELISA Kit - high sensitivity (AB241383)

Interpolated concentrations of native Fibrinogen in human serum and plasma samples.

The concentrations of Fibrinogen were measured in duplicates, interpolated from the Fibrinogen standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 1 : 670, plasma (citrate) 1 : 5x105, plasma (EDTA) 1 : 5x105, and plasma (heparin) 1 : 5x105. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Fibrinogen concentration was determined to be 3.45 μg/mL in serum, 2.86 mg/mL in plasma (citrate), 2.96 mg/mL in plasma (EDTA), and 2.64 mg/mL in plasma (heparin).

Key facts

Detection method

Colorimetric

Sample types

Saliva, Urine, Heparin Plasma, Citrate plasma, Cell culture supernatant, Milk, Serum, EDTA Plasma

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 29 pg/mL

Range

125 - 8000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

You may be interested in:

AB256605

Human Fibrinogen Antibody Pair - BSA and Azide free - high sensitivity

0

0 Reviews

View product

We recommend this product because it’s often used in the same experiment or related research.

We advise that you always check the datasheet to ensure it fits your experiments, or contact ourtechnical teamfor help.

style
left-column

Product details

Human Fibrinogen ELISA Kit - high sensitivity ab241383 is a rapid single-wash 90-min ELISA kit to measure Human Fibrinogen in saliva, urine, cell culture supernatant, milk, plasma and serum. This SimpleStep ELISA® sensitivity is 29 pg/mL.

How the assay works
Human Fibrinogen SimpleStep ELISA® employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Assay Specificity
This kit recognizes both native and recombinant human Fibrinogen protein in serum, plasma, urine, saliva, milk, and cell culture supernatant only. Cell and tissue extract samples have not been tested with this kit.

Human Fibrinogen ELISA Kit - high sensitivity ab241383 protocol summary:
1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate for 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add stop solution
5. Read the results on a plate reader at 450nm

How other researchers are using Human Fibrinogen ELISA Kit - high sensitivity ab241383
Human Fibrinogen ELISA Kit - high sensitivity ab241383 has been used to study fibrinogen function and the effects of a novel mutation on fibinogen synthesis, secretion, and polymerization(1), extracorporeal bioengineered liver grafts for treating acute liver failure(2), and CRSPR/Cas9 to upregulate expression of coagulation factors II, VII, IX, X, and fibrinogen(3).

References:
(1)Fei Chen et al. 2024, PMID: 38233949,
(2)Jeff J Ross et al. 2023, PMID: 37892931,
(3)Mark J Osborn et al. 2022, PMID: 35563479

Save your precious samples
Our 384-well Human Fibrinogen ELISA Kit - high sensitivity only requires a maxmum of 12.5µL

Design your own immunoassay
We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation
- Human Fibrinogen Antibody Pair - BSA and Azide free - high sensitivity ab256605
- Anti-Fibrinogen antibody [EPR19765-189] - BSA and Azide free (Capture) ab244636
- Anti-Fibrinogen antibody [EPR19765-63] - BSA and Azide free (Detector) ab244897

Related and recommended products
Human Fibrinogen ELISA Kit - high sensitivity ab241383 is commonly used to study fibrinogen levels in coagulation, fibrinolysis, and liver function.

Other ELISA kits to measure coagulation and liver health include:
- Human AST ELISA Kit (Aspartate Aminotransferase) ab263881
- Human PAI1 ELISA Kit (SERPINE1) ab269373
- Human Plasminogen ELISA Kit (PLG) ab108893

style
left-column

Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "11.7" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.4" } ]
style
left-column

Recovery

[ { "sample": "Cell culture supernatant", "range": "93 - 95 %", "average": "= 94" }, { "sample": "Milk", "range": "102 - 105 %", "average": "= 103" }, { "sample": "Serum", "range": "90 - 94 %", "average": "= 91" }, { "sample": "EDTA Plasma", "range": "91 - 94 %", "average": "= 93" }, { "sample": "Urine", "range": "99 - 100 %", "average": "= 100" }, { "sample": "Saliva", "range": "98 - 104 %", "average": "= 101" }, { "sample": "Heparin Plasma", "range": null, "average": "= 95" }, { "sample": "Citrate plasma", "range": "91 - 96 %", "average": "= 94" } ]
style
left-column

What's included?

{ "values": { "1x384Tests": { "sellingSize": "1 x 384 Tests", "publicAssetCode":"ab241383-1x384Tests", "assetComponentDetails": [ { "size":"2 x 12 mL", "name":"Stop Solution", "number":"AB241383-CMP03" }, { "size":"2 x 1 Vial", "name":"Human Fibrinogen Lyophilized Purified Protein", "number":"AB241383-CMP07" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB241383-CMP10" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BI", "number":"AB241383-CMP08" }, { "size":"1 x 250 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB241383-CMP01" }, { "size":"1 x 600 µL", "name":"10X Human Fibrinogen Capture Antibody", "number":"AB241383-CMP05" }, { "size":"1 x 1 Unit", "name":"384 well CaptSure™ microplates", "number":"AB241383-CMP11" }, { "size":"2 x 12 mL", "name":"TMB Development Solution", "number":"AB241383-CMP04" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB241383-CMP09" }, { "size":"1 x 600 µL", "name":"10X Human Fibrinogen Detector Antibody", "number":"AB241383-CMP06" } ] }, "10x96Tests": { "sellingSize": "10 x 96 Tests", "publicAssetCode":"ab241383-10x96Tests", "assetComponentDetails": [ { "size":"1 x 120 mL", "name":"Stop Solution", "number":"AB241383-CMP03" }, { "size":"1 x 200 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB241383-CMP10" }, { "size":"1 x 120 mL", "name":"TMB Development Solution", "number":"AB241383-CMP04" }, { "size":"10 x 6 mL", "name":"Antibody Diluent 4BI", "number":"AB241383-CMP08" }, { "size":"2 x 10 Vial", "name":"Human Fibrinogen Lyophilized Purified Protein", "number":"AB241383-CMP07" }, { "size":"1 x 6000 µL", "name":"10X Human Fibrinogen Capture Antibody", "number":"AB241383-CMP05" }, { "size":"2 x 250 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB241383-CMP01" }, { "size":"1 x 6000 µL", "name":"10X Human Fibrinogen Detector Antibody", "number":"AB241383-CMP06" }, { "size":"10 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB241383-CMP02" }, { "size":"10 x 1 Unit", "name":"Plate Seals", "number":"AB241383-CMP09" } ] }, "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab241383-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB241383-CMP03", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Fibrinogen Capture Antibody", "number":"AB241383-CMP05", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB241383-CMP10", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB241383-CMP01", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BI", "number":"AB241383-CMP08", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human Fibrinogen Lyophilized Purified Protein", "number":"AB241383-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB241383-CMP02", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB241383-CMP09", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Fibrinogen Detector Antibody", "number":"AB241383-CMP06", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB241383-CMP04", "productcode":"" } ] } } }
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
style
left-column
style
left-column

Product protocols

style
left-column

Target data

Cleaved by the protease thrombin to yield monomers which, together with fibrinogen beta (FGB) and fibrinogen gamma (FGG), polymerize to form an insoluble fibrin matrix. Fibrin has a major function in hemostasis as one of the primary components of blood clots. In addition, functions during the early stages of wound repair to stabilize the lesion and guide cell migration during re-epithelialization. Was originally thought to be essential for platelet aggregation, based on in vitro studies using anticoagulated blood. However, subsequent studies have shown that it is not absolutely required for thrombus formation in vivo. Enhances expression of SELP in activated platelets via an ITGB3-dependent pathway. Maternal fibrinogen is essential for successful pregnancy. Fibrin deposition is also associated with infection, where it protects against IFNG-mediated hemorrhage. May also facilitate the immune response via both innate and T-cell mediated pathways.
See full target information FGA
style
left-column

Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Hereditas 161:4 PubMed38233949

2024

A novel missense mutation (FGG c.1168G > T) in the gamma chain of fibrinogen causing congenital hypodysfibrinogenemia with bleeding phenotype.

Applications

Unspecified application

Species

Unspecified reactive species

Nuo Xu,Liping Zheng,Zhehao Dai,Jun Zhu,Peng Xie,Shun Yang,Fei Chen

Bioengineering (Basel, Switzerland) 10: PubMed37892931

2023

Manufacturing and Functional Characterization of Bioengineered Liver Grafts for Extracorporeal Liver Assistance in Acute Liver Failure.

Applications

Unspecified application

Species

Unspecified reactive species

Victoria L Nelson,Aron R Stumbras,R Noelle Palumbo,Shawn A Riesgraf,Marie S Balboa,Zachary A Hannah,Isaac J Bergstrom,Christopher J Fecteau,John R Lake,John J Barry,Jeff J Ross

International journal of molecular sciences 24: PubMed37239814

2023

Engineering Human Cells Expressing CRISPR/Cas9-Synergistic Activation Mediators for Recombinant Protein Production.

Applications

Unspecified application

Species

Unspecified reactive species

Colby J Feser,James M Williams,Daniel T Lammers,Jason R Bingham,Matthew J Eckert,Jakub Tolar,Mark J Osborn

International journal of molecular sciences 23: PubMed36142345

2022

SARS-CoV-2 Spike Proteins and Cell-Cell Communication Inhibits TFPI and Induces Thrombogenic Factors in Human Lung Microvascular Endothelial Cells and Neutrophils: Implications for COVID-19 Coagulopathy Pathogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Biju Bhargavan,Georgette D Kanmogne

International journal of hematology 116:937-946 PubMed35994163

2022

Plasma biomarkers associated with survival and thrombosis in hospitalized COVID-19 patients.

Applications

Unspecified application

Species

Unspecified reactive species

David Cabrera-Garcia,Andrea Miltiades,Peter Yim,Samantha Parsons,Katerina Elisman,Mohammad Taghi Mansouri,Gebhard Wagener,Neil L Harrison

International journal of molecular sciences 23: PubMed35563479

2022

Engineering CRISPR/Cas9 for Multiplexed Recombinant Coagulation Factor Production.

Applications

Unspecified application

Species

Unspecified reactive species

Colby J Feser,Christopher J Lees,Daniel T Lammers,Megan J Riddle,Jason R Bingham,Matthew J Eckert,Jakub Tolar,Mark J Osborn
View all publications
style
left-column
style
left-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
style
left-column
websiteProtocolBooklet
en
style
right-column
style
bg-slate-100

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com