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AB309310

Human Fibrinogen gamma chain ELISA Kit - Extracellular

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Human Fibrinogen gamma chain ELISA Kit - Extracellular is a Sandwich ELISA for the measurement of Human Fibrinogen gamma chain - Extracellular in Human in Biofluids, Cell Culture Media samples.
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Sandwich ELISA - Human Fibrinogen gamma chain ELISA Kit - Extracellular (AB309310)
  • sELISA

Supplier Data

Sandwich ELISA - Human Fibrinogen gamma chain ELISA Kit - Extracellular (AB309310)

Interpolated concentrations of Fibrinogen gamma chain in normal Human serum donors

Serum of ten individual healthy Human female donors was measured in duplicate. Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Fibrinogen gamma chain concentration was determined to be 1145.5 ng/mL with a range of 255 – 2726 ng/mL.

Sandwich ELISA - Human Fibrinogen gamma chain ELISA Kit - Extracellular (AB309310)
  • sELISA

Supplier Data

Sandwich ELISA - Human Fibrinogen gamma chain ELISA Kit - Extracellular (AB309310)

Interpolated concentrations of Human Fibrinogen gamma chain in Human plasma (citrate), plasma (EDTA), and plasma (heparin)

Interpolated concentration of native Fibrinogen gamma chain was measured in duplicate at different sample concentrations. Undiluted samples are 1 : 800,000 plasma (citrate), plasma (EDTA), and plasma (heparin). The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

Sandwich ELISA - Human Fibrinogen gamma chain ELISA Kit - Extracellular (AB309310)
  • sELISA

Supplier Data

Sandwich ELISA - Human Fibrinogen gamma chain ELISA Kit - Extracellular (AB309310)

Interpolated concentrations of Human Fibrinogen gamma chain in serum and HepG2 cell culture supernatant

Interpolated concentration of native Fibrinogen gamma chain was measured in duplicate at different sample concentrations. Undiluted samples are as follows : serum 1 : 1,000 and HepG2 supernatant 1 : 250. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

Sandwich ELISA - Human Fibrinogen gamma chain ELISA Kit - Extracellular (AB309310)
  • sELISA

Supplier Data

Sandwich ELISA - Human Fibrinogen gamma chain ELISA Kit - Extracellular (AB309310)

Example of Human Fibrinogen gamma chain standard curve in Sample Diluent NS. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Serum, Citrate plasma, EDTA Plasma, Heparin Plasma, Cell culture supernatant

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 8.7 pg/mL

Range

35.938 - 2300 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human Fibrinogen gamma chain - Extracellular SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Fibrinogen gamma chain - Extracellular protein in human serum, plasma and cell culture supernatant. Quantitate Human Fibrinogen gamma chain - Extracellular with 8.7 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

CROSS  REACTIVITY

2,300 pg/mL of recombinant Human Fibrinogen alpha was tested for cross reactivity. No cross reactivity was observed.

2,300 pg/mL of recombinant Human Fibrinogen beta was tested for cross reactivity. <3% cross reactivity was observed.

INTERFERENCE

575 pg/mL of recombinant Human Fibrinogen alpha and 575 pg/mL of recombinant Human Fibrinogen beta was tested for interference with 575 pg/mL of recombinant Human Fibrinogen gamma. No interference was observed. 575 pg/mL of recombinant Human Fibrinogen beta increased interpolated value of 575 pg/mL of recombinant Human Fibrinogen gamma 5.7%.

SPECIES REACTIVITY

Other species reactivity was determined by measuring 1:1000 serum samples of various species, interpolating the protein concentrations from the species standard curve, and expressing the interpolated concentrations as a percentage of the protein concentration in species serum assayed at the same dilution.

Reactivity of 77% was determined for primate serum.

No signal was observed in 1:1000 serum samples from the following species: Mouse, Rat, Bovine

Other species reactivity not determined.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Plasma", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "1.9" }, { "reproducibilityType": "Inter", "sample": "Plasma", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "1" } ]
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Recovery

[ { "sample": "Serum", "range": "98 - 110 %", "average": "= 103" }, { "sample": "Citrate plasma", "range": "85 - 96 %", "average": "= 90" }, { "sample": "EDTA Plasma", "range": "95 - 103 %", "average": "= 100" }, { "sample": "Heparin Plasma", "range": "95 - 110 %", "average": "= 103" }, { "sample": "Cell culture supernatant", "range": "101 - 113 %", "average": "= 106" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

Together with fibrinogen alpha (FGA) and fibrinogen beta (FGB), polymerizes to form an insoluble fibrin matrix. Has a major function in hemostasis as one of the primary components of blood clots. In addition, functions during the early stages of wound repair to stabilize the lesion and guide cell migration during re-epithelialization. Was originally thought to be essential for platelet aggregation, based on in vitro studies using anticoagulated blood. However, subsequent studies have shown that it is not absolutely required for thrombus formation in vivo. Enhances expression of SELP in activated platelets via an ITGB3-dependent pathway. Maternal fibrinogen is essential for successful pregnancy. Fibrin deposition is also associated with infection, where it protects against IFNG-mediated hemorrhage. May also facilitate the antibacterial immune response via both innate and T-cell mediated pathways.
See full target information FGG

Alternative Names

PRO2061, FGG, Fibrinogen gamma chain
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websiteProtocolBooklet
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Product promise

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