JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB108847

Human Fibronectin ELISA Kit

Be the first to review this product! Submit a review

|

(6 Publications)

Human Fibronectin ELISA Kit is a Sandwich ELISA for the measurement of Human Fibronectin in Human in Biofluids samples.

View Alternative Names

FN, FN1, Fibronectin, Cold-insoluble globulin, CIG

2 Images
Sandwich ELISA - Human Fibronectin ELISA Kit (AB108847)
  • sELISA

Unknown

Sandwich ELISA - Human Fibronectin ELISA Kit (AB108847)

Fibronectin measured in biological fluids and cell culture supernatants showing quantity (ug) per mL of tested sample. Human urine diluted 1-3 fold. Human saliva diluted 10-100 fold. Human milk diluted 100-1000 fold. Cell supernatants diluted 10-1000 fold.

Sandwich ELISA - Human Fibronectin ELISA Kit (AB108847)
  • sELISA

Unknown

Sandwich ELISA - Human Fibronectin ELISA Kit (AB108847)

Standard curve : mean of duplicates (+/- SD) with background reads subtracted

Key facts

Detection method

Colorimetric

Sample types

Cerebral Spinal Fluid, Saliva, Urine, Milk

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 4 ng/mL

Range

0.004 - 1 µg/mL

Assay time

3h 30m

Assay Platform

Microplate

style
left-column

Complementary Products

Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Assay Kits

AB210966

Human Pro-Collagen I alpha 1 ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Human Pro-Collagen I alpha 1 ELISA Kit (AB210966)

  • 5
  • 1
Assay Kits

AB214025

Human IL-1 beta ELISA Kit

KO Validated|Recombinant|SimpleStep

Sandwich ELISA - Human IL-1 beta ELISA Kit (AB214025)

  • 4
  • 1
Assay Kits

AB241383

Human Fibrinogen ELISA Kit - high sensitivity

Recombinant|SimpleStep

ELISA - Human Fibrinogen ELISA Kit - high sensitivity (AB241383)

  • 0
  • 0
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

Product details

Abcam's Fibronectin Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Fibronectin levels in milk, urine, saliva, CSF, cell culture, cell lysate, and tissue samples.

A Fibronectin specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Fibronectin specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Fibronectin captured in plate.

Get results in 90 minutes with Human Fibronectin ELISA Kit (ab181419) from our SimpleStep ELISA® range.

The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.

style
left-column

Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "7" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.9" } ]
style
left-column

What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab108847-1x96Tests", "assetComponentDetails": [ { "size":"2 x 30 mL", "name":"20X Wash Buffer Concentrate", "number":"AB108847-CMP06", "productcode":"" }, { "size":"1 x 30 mL", "name":"10X Diluent N Concentrate", "number":"AB108847-CMP02", "productcode":"" }, { "size":"1 x 80 µL", "name":"100X Streptavidin-Peroxidase Conjugate", "number":"AB108847-CMP09", "productcode":"" }, { "size":"1 x 7 mL", "name":"Chromogen Substrate", "number":"AB108847-CMP03", "productcode":"" }, { "size":"3 x 1 Unit", "name":"Sealing Tapes", "number":"AB108847-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Fibronectin Microplate (12 x 8 well strips)", "number":"AB108847-CMP04", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Fibronectin Standard", "number":"AB108847-CMP05", "productcode":"" }, { "size":"1 x 11 mL", "name":"Stop Solution", "number":"AB108847-CMP08", "productcode":"" }, { "size":"1 x 120 µL", "name":"50X Biotinylated Human Fibronectin Antibody", "number":"AB108847-CMP01", "productcode":"" } ] } } }
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
Multi
Storage information
Please refer to protocols
style
left-column
style
left-column

Product protocols

style
left-column

Target data

Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin (PubMed : 3024962, PubMed : 3593230, PubMed : 3900070, PubMed : 7989369). Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape (PubMed : 3024962, PubMed : 3593230, PubMed : 3900070, PubMed : 7989369). Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization (By similarity). Participates in the regulation of type I collagen deposition by osteoblasts (By similarity). Acts as a ligand for the LILRB4 receptor, inhibiting FCGR1A/CD64-mediated monocyte activation (PubMed : 34089617).. Anastellin. Binds fibronectin and induces fibril formation. This fibronectin polymer, named superfibronectin, exhibits enhanced adhesive properties. Both anastellin and superfibronectin inhibit tumor growth, angiogenesis and metastasis. Anastellin activates p38 MAPK and inhibits lysophospholipid signaling.. Secreted by contracting muscle, induces liver autophagy, a degradative pathway for nutrient mobilization and damage removal, and systemic insulin sensitization via hepatic ITGA5 : ITGB1 integrin receptor signaling.
See full target information FN1
style
left-column

Publications (6)

Recent publications for all applications. Explore the full list and refine your search

NPJ Parkinson's disease 11:1 PubMed39747089

2025

Plasma fibronectin is a prognostic biomarker of disability in Parkinson's disease: a prospective, multicenter cohort study.

Applications

Unspecified application

Species

Unspecified reactive species

Shuzhen Zhu,Hualin Li,Zifeng Huang,Yiheng Zeng,Jianmin Huang,Guixia Li,Shujuan Yang,Hang Zhou,Zihan Chang,Zhenchao Xie,Rongfang Que,Xiaobo Wei,Minzi Li,Yanran Liang,Wenbiao Xian,Mengyan Li,Ying Pan,Fanheng Huang,Lin Shi,Chengwu Yang,Chao Deng,Lucia Batzu,Karolina Poplawska-Domaszewicz,Shuhan Chen,Ling-Ling Chan,K Ray Chaudhuri,Eng-King Tan,Qing Wang

Cancer science 109:3285-3293 PubMed30024080

2018

Curcumin analog, GO-Y078, overcomes resistance to tumor angiogenesis inhibitors.

Applications

Unspecified application

Species

Unspecified reactive species

Kazuhiro Shimazu,Masahiro Inoue,Shunsuke Sugiyama,Koji Fukuda,Taichi Yoshida,Daiki Taguchi,Yoshihiko Uehara,Sei Kuriyama,Masamitsu Tanaka,Masatomo Miura,Hiroshi Nanjyo,Yoshiharu Iwabuchi,Hiroyuki Shibata

Scientific reports 7:42461 PubMed28198381

2017

Bio-functionalized magnetic nanoparticles for the immunoassay of fetal fibronectin: a feasibility study for the prediction of preterm birth.

Applications

ELISA

Species

Human

Chian-Huey Wong,Chie-Pein Chen,Chia-Chen Chang,Chen-Yu Chen

Molecular medicine reports 12:1356-62 PubMed25815703

2015

Fetuin-A 742 (C/T) and 766 (C/G) polymorphic sites are associated with increased risk of myocardial infarction in older patients (≥ 40 years of age).

Applications

Unspecified application

Species

Unspecified reactive species

Ajda Coker-Gurkan,Deniz Coskun,Elif Damla Arisan,Pinar Obakan,Özer Soylu,Narcin Palavan Unsal

International journal of cosmetic science 37:41-55 PubMed25256953

2014

Cryptomphalus aspersa mollusc eggs extract promotes migration and prevents cutaneous ageing in keratinocytes and dermal fibroblasts in vitro.

Applications

Unspecified application

Species

Unspecified reactive species

J Espada,M Matabuena,N Salazar,S Lucena,O Kourani,E Carrasco,M Calvo,C Rodríguez,E Reyes,S González,A Juarranz

Oncogene 34:455-64 PubMed24561527

2014

Transcriptional regulation of fibronectin by p21-activated kinase-1 modulates pancreatic tumorigenesis.

Applications

Unspecified application

Species

Unspecified reactive species

S Jagadeeshan,Y R Krishnamoorthy,M Singhal,A Subramanian,J Mavuluri,A Lakshmi,A Roshini,G Baskar,M Ravi,L D Joseph,K Sadasivan,A Krishnan,A S Nair,G Venkatraman,S K Rayala
View all publications
style
left-column
style
left-column
websiteProtocolBooklet
en
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com