Human Fibronectin ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Human Fibronectin in Human in Cerebral Spinal Fluid, Saliva, Urine, Milk samples.
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- Cancer science 109:3285-32932018Curcumin analog, GO-Y078, overcomes resistance to tumor angiogenesis inhibitors.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKazuhiro Shimazu et. al.PubMed 30024080
- Scientific reports 7:424612017Bio-functionalized magnetic nanoparticles for the immunoassay of fetal fibronectin: a feasibility study for the prediction of preterm birth.Applications:ELISAReactive species:HumanChian-Huey Wong,Chie-Pein Chen,Chia-Chen Chang,Chen-Yu ChenPubMed 28198381
- Molecular medicine reports 12:1356-622015Fetuin-A 742 (C/T) and 766 (C/G) polymorphic sites are associated with increased risk of myocardial infarction in older patients (≥ 40 years of age).Applications:Unspecified applicationReactive species:Unspecified reactive speciesAjda Coker-Gurkan et. al.PubMed 25815703
- International journal of cosmetic science 37:41-552014Cryptomphalus aspersa mollusc eggs extract promotes migration and prevents cutaneous ageing in keratinocytes and dermal fibroblasts in vitro.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJ Espada,M Matabuena,N Salazar,S Lucena,O Kourani,E Carrasco,M Calvo,C Rodríguez,E Reyes,S González,A JuarranzPubMed 25256953
- Oncogene 34:455-642014Transcriptional regulation of fibronectin by p21-activated kinase-1 modulates pancreatic tumorigenesis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesS Jagadeeshan et. al.PubMed 24561527
Key facts
- Detection method
- Colorimetric
- Sample types
- Cerebral Spinal Fluid, Saliva, Urine, Milk
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 0.004 - 1 µg/mL
- Assay time
- 3h 30m
- Sensitivity
- = 4 ng/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin (PubMed:3024962, PubMed:3593230, PubMed:3900070, PubMed:7989369). Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape (PubMed:3024962, PubMed:3593230, PubMed:3900070, PubMed:7989369). Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization (By similarity). Participates in the regulation of type I collagen deposition by osteoblasts (By similarity). Acts as a ligand for the LILRB4 receptor, inhibiting FCGR1A/CD64-mediated monocyte activation (PubMed:34089617). Anastellin. Binds fibronectin and induces fibril formation. This fibronectin polymer, named superfibronectin, exhibits enhanced adhesive properties. Both anastellin and superfibronectin inhibit tumor growth, angiogenesis and metastasis. Anastellin activates p38 MAPK and inhibits lysophospholipid signaling. Secreted by contracting muscle, induces liver autophagy, a degradative pathway for nutrient mobilization and damage removal, and systemic insulin sensitization via hepatic ITGA5:ITGB1 integrin receptor signaling.
Alternative names
FN, FN1, Fibronectin, Cold-insoluble globulin, CIG
What's included?
Recommended products
Human Fibronectin ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Human Fibronectin in Human in Cerebral Spinal Fluid, Saliva, Urine, Milk samples.
Key facts
- Detection method
- Colorimetric
- Sample types
- Cerebral Spinal Fluid, Saliva, Urine, Milk
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 0.004 - 1 µg/mL
- Assay time
- 3h 30m
- Assay Platform
- Microplate
- Sensitivity
- = 4 ng/mL
- General recovery
- 97.5%
Precision
Intra assay
| Sample | n | C.V. |
|---|---|---|
Sample Overall | n 0 | C.V. 4.9 |
Inter assay
| Sample | n | C.V. |
|---|---|---|
Sample Overall | n 0 | C.V. 7 |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- Multi
- Storage information
- Please refer to protocols
Notes
Abcam's Fibronectin Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Fibronectin levels in milk, urine, saliva, CSF, cell culture, cell lysate, and tissue samples.
A Fibronectin specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Fibronectin specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Fibronectin captured in plate.
Get results in 90 minutes with Human Fibronectin ELISA Kit (ab181419) from our SimpleStep ELISA® range.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
Sandwich ELISA - Human Fibronectin ELISA Kit (ab108847)
Standard curve: mean of duplicates (+/- SD) with background reads subtracted
Sandwich ELISA - Human Fibronectin ELISA Kit (ab108847)
Fibronectin measured in biological fluids and cell culture supernatants showing quantity (ug) per mL of tested sample. Human urine diluted 1-3 fold. Human saliva diluted 10-100 fold. Human milk diluted 100-1000 fold. Cell supernatants diluted 10-1000 fold.
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Product protocols
- Visit the general protocols
- Visit troubleshooting
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