Human Fibronectin ELISA Kit, Fluorescent

• sELISA

Supplier Data

Sandwich ELISA - Human Fibronectin ELISA Kit, Fluorescent (AB229398)

Interpolated concentrations of native Fibronectin in human cell and tissue extract samples.

The concentrations of Fibronectin were measured in three different dilutions in duplicate and interpolated from the Fibronectin standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted in ng of Fibronectin per mg of extract (mean +/- SD, n=3). Fibronectin concentration was determined to be 271.8 ng/mg in HepG2 cell extract, 65.52 ng/mg in SW 480 cell extract, 359.6 ng/mg in U87-MG cell extract, 75.22 ng/mg in liver tissue extract, 47.20 ng/mg in heart tissue extract, 43.52 ng/mg in skeletal muscle tissue extract, 557.9 ng/mg in colon tissue extract, and 998.8 ng/mg in placenta tissue extract samples.

• sELISA

Supplier Data

Sandwich ELISA - Human Fibronectin ELISA Kit, Fluorescent (AB229398)

Example of human Fibronectin standard curve in Sample Diluent NS.

Background-subtracted data values (mean +/- SD) are graphed.

• sELISA

Supplier Data

Sandwich ELISA - Human Fibronectin ELISA Kit, Fluorescent (AB229398)

Serum from ten individual healthy male human donors was measured in duplicate.

Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Fibronectin concentration was determined to be 146.2 μg/mL with a range of 35.99 – 319.2 μg/mL.

• sELISA

Unknown

Sandwich ELISA - Human Fibronectin ELISA Kit, Fluorescent (AB229398)

Interpolated concentrations of native Fibronectin in human HepG2 cell extract samples based on a 25 μg/mL extract load, SW 480 cell extract samples based on a 100 μg/mL extract load, and U87-MG cell extract samples based on a 5 μg/mL extract load. The concentrations of Fibronectin were measured in duplicate and interpolated from the Fibronectin standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Fibronectin concentration was determined to be 7085 pg/mL in HepG2 cell extract, 6314 pg/mL in SW 480 cell extract, and 1749 pg/mL in U87-MG cell extract.

• sELISA

Supplier Data

Sandwich ELISA - Human Fibronectin ELISA Kit, Fluorescent (AB229398)

Interpolated concentrations of native Fibronectin in human breast milk and HepG2 cell culture supernatant (4 days) samples.

The concentrations of Fibronectin were measured in duplicates, interpolated from the Fibronectin standard curves and corrected for sample dilution. Undiluted samples are as follows : milk 1/100, and HepG2 cell culture supernatant 1/2000. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Fibronectin concentration was determined to be 502.9 ng/mL in milk, and 9683 ng/mL in HepG2 cell culture supernatant.

• sELISA

Supplier Data

Sandwich ELISA - Human Fibronectin ELISA Kit, Fluorescent (AB229398)

Interpolated concentrations of native Fibronectin in human serum and plasma samples.

The concentrations of Fibronectin were measured in duplicates, interpolated from the Fibronectin standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 1/20,000, plasma (citrate) 1/20,000, and plasma (EDTA) 1/40,000. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Fibronectin concentration was determined to be 126.5 μg/mL in serum, 141.5 μg/mL in plasma (citrate), and 232.1 μg/mL in plasma (EDTA).

• sELISA

Supplier Data

Sandwich ELISA - Human Fibronectin ELISA Kit, Fluorescent (AB229398)

Interpolated concentrations of native Fibronectin in human liver tissue extract based on a 100 μg/mL extract load, heart tissue extract based on a 100 μg/mL extract load, skeletal muscle based on a 100 μg/mL extract load, colon tissue extract based on a 5 μg/mL extract load, and placenta tissue extract based on a 1.25 μg/mL extract load. The concentrations of Fibronectin were measured in duplicate and interpolated from the Fibronectin standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Fibronectin concentration was determined to be 7634 pg/mL in liver tissue extract, 4910 pg/mL in heart tissue extract, 4278 pg/mL in skeletal muscle tissue extract, 2684 pg/mL in colon tissue extract, and 1224 pg/mL in placenta tissue extract.