JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB213779

Human Ficolin-3 ELISA Kit

Be the first to review this product! Submit a review

|

(1 Publication)

Human Ficolin-3 ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human Ficolin-3 in Human in Cell Culture Media, Biofluids samples.

View Alternative Names

FCNH, HAKA1, FCN3, Ficolin-3, Collagen/fibrinogen domain-containing lectin 3 p35, Collagen/fibrinogen domain-containing protein 3, Hakata antigen

1 Images
Sandwich ELISA - Human Ficolin-3 ELISA Kit (AB213779)
  • sELISA

Supplier Data

Sandwich ELISA - Human Ficolin-3 ELISA Kit (AB213779)

Human Ficolin-3 ELISA Kit (ab213779) Standard Curve.

Key facts

Detection method

Colorimetric

Sample types

Cell culture supernatant, Heparin Plasma, Serum

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

< 20 pg/mL

Range

0.78 - 50 ng/mL

Assay time

3h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

Product details

The Human Ficolin-3 Enzyme-Linked Immunosorbent Assay (ELISA) kit (ab213779) is designed for the quantitative measurement of Human Ficolin-3 in cell culture supernatants, serum and plasma (heparin).

The ELISA kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Ficolin-3 has been pre-coated onto 96-well plates. Standards (Expression system for standard: NSO; Immunogen sequence: Q24-R299) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Ficolin-3 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB is used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human Ficolin-3 amount of sample captured in plate.

Ficolin-3, also known as HAKA1, is a protein that in humans is encoded by the FCN3 gene. It is mapped to 1p36.11. The protein can activate the complement pathway in association with MASPs and sMAP, thereby aiding in host defense through the activation of the lectin pathway. Ficolin-3 is expressed as a 35-kD protein that reacts with systemic lupus erythematosus sera. Ficolin-3 does not bind fibronectin (FN1), elastin (ELN), or zymosan, and the lectin activity of the Ficolin-3 is calcium independent. Homozygosity for the variant in the FCN3 gene results in a recessive complement deficiency syndrome.

style
left-column

Precision

[ { "reproducibilityType": "Inter", "sample": "Sample 3", "replicates": 24, "mean": "25.739 ng/mL", "standardDeviation": "1.853", "coefficientOfVariability": "= 7.2" }, { "reproducibilityType": "Intra", "sample": "Sample 1", "replicates": 16, "mean": "1.216 ng/mL", "standardDeviation": "0.08", "coefficientOfVariability": "= 6.6" }, { "reproducibilityType": "Inter", "sample": "Sample 2", "replicates": 24, "mean": "6.253 ng/mL", "standardDeviation": "0.45", "coefficientOfVariability": "= 7.2" }, { "reproducibilityType": "Intra", "sample": "Sample 3", "replicates": 16, "mean": "26.48 ng/mL", "standardDeviation": "1.403", "coefficientOfVariability": "= 5.3" }, { "reproducibilityType": "Intra", "sample": "Sample 2", "replicates": 16, "mean": "5.741 ng/mL", "standardDeviation": "0.396", "coefficientOfVariability": "= 6.9" }, { "reproducibilityType": "Inter", "sample": "Sample 1", "replicates": 24, "mean": "1.219 ng/mL", "standardDeviation": "0.08", "coefficientOfVariability": "= 6.6" } ]
style
left-column

What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab213779-1x96Tests", "assetComponentDetails": [ { "size":"1 x 100 µL", "name":"Avidin-Biotin-Peroxidase Complex (ABC)", "number":"AB213779-CMP10", "productcode":"" }, { "size":"1 x 100 µL", "name":"Biotinylated anti- Human Ficolin-3 antibody", "number":"AB213779-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Anti-Human Ficolin-3 coated Microplate (12 x 8 wells)", "number":"AB213779-CMP09", "productcode":"" }, { "size":"1 x 10 mL", "name":"TMB Color Developing Agent", "number":"AB213779-CMP04", "productcode":"" }, { "size":"1 x 12 mL", "name":"Antibody Diluent Buffer", "number":"AB213779-CMP08", "productcode":"" }, { "size":"1 x 10 mL", "name":"TMB Stop Solution", "number":"AB213779-CMP05", "productcode":"" }, { "size":"1 x 30 mL", "name":"Sample Diluent Buffer", "number":"AB213779-CMP02", "productcode":"" }, { "size":"4 x 1 Unit", "name":"Adhesive Plate Seal", "number":"AB213779-CMP06", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Lyophilized recombinant Human Ficolin-3 standard", "number":"AB213779-CMP01", "productcode":"" }, { "size":"1 x 20 mL", "name":"Wash Buffer (25X)", "number":"AB213779-CMP11", "productcode":"" }, { "size":"1 x 12 mL", "name":"ABC Diluent Buffer", "number":"AB213779-CMP03", "productcode":"" } ] } } }
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

FCN3 also known as ficolin-3 is a multifunctional protein recognized for its role in the immune system. Ficolin-3 is approximately 35 kDa in mass and is mainly expressed in the liver though it can also be found in various other tissues such as the spleen and lungs. It belongs to the ficolin family of proteins which are involved in innate immunity. The protein features a collagen-like domain and a fibrinogen-like domain facilitating its binding to sugars on microbial surfaces.
Biological function summary

Ficolin-3 acts as an essential player in the immune response contributing to pathogen recognition and clearance. It forms a complex with other components to activate the complement pathway particularly the lectin pathway. Ficolin-3 binds to carbohydrate patterns on the surface of pathogens leading to opsonization and clearance. This activity highlights its function as a defense mechanism against infections and its role in recognizing altered cells.

Pathways

Ficolin-3 facilitates the activation of the lectin complement pathway. This pathway is important for innate immune defense and involves several other proteins including mannose-binding lectin (MBL) and MASP-2 which are connected with ficolin-3 in mediating complement activation. The engagement of ficolin-3 in this pathway highlights its importance in identifying and eliminating pathogens effectively.

Mutations or deficiencies in the function of ficolin-3 are associated with an increased risk of infections and autoimmune diseases such as systemic lupus erythematosus (SLE). The protein's role in immune defense makes it a critical factor in maintaining a balanced immune response. Additionally related proteins like MBL can also influence the progression or predisposition to these conditions further emphasizing the interconnectedness of ficolin-3 within immune regulatory networks.
style
left-column

Product protocols

style
left-column

Target data

May function in innate immunity through activation of the lectin complement pathway. Calcium-dependent and GlcNAc-binding lectin. Has affinity with GalNAc, GlcNAc, D-fucose, as mono/oligosaccharide and lipopolysaccharides from S.typhimurium and S.minnesota.
See full target information FCN3
style
left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Molecular medicine (Cambridge, Mass.) 27:129 PubMed34663207

2021

CXCL10 levels at hospital admission predict COVID-19 outcome: hierarchical assessment of 53 putative inflammatory biomarkers in an observational study.

Applications

Unspecified application

Species

Unspecified reactive species

Nicola I Lorè,Rebecca De Lorenzo,Paola M V Rancoita,Federica Cugnata,Alessandra Agresti,Francesco Benedetti,Marco E Bianchi,Chiara Bonini,Annalisa Capobianco,Caterina Conte,Angelo Corti,Roberto Furlan,Paola Mantegani,Norma Maugeri,Clara Sciorati,Fabio Saliu,Laura Silvestri,Cristina Tresoldi,Fabio Ciceri,Patrizia Rovere-Querini,Clelia Di Serio,Daniela M Cirillo,Angelo A Manfredi
View all publications
style
left-column
style
left-column
websiteProtocolBooklet
en
style
right-column

Complementary Products

Assay Kits

AB278121

Human MASP2 ELISA Kit

Recombinant|SimpleStep

ELISA - Human MASP2 ELISA Kit (AB278121)

  • 0
  • 0
Assay Kits

AB269372

Human Fetuin-A ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Human Fetuin-A ELISA Kit (AB269372)

  • 0
  • 0
Assay Kits

AB213832

Human Tetranectin ELISA Kit (CLEC3B)

Sandwich ELISA - Human Tetranectin ELISA Kit (CLEC3B) (AB213832)

  • 0
  • 0
Assay Kits

AB196264

Human Retinol Binding Protein 4 ELISA Kit

Recombinant|SimpleStep

ELISA - Human Retinol Binding Protein 4 ELISA Kit (AB196264)

  • 0
  • 0

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com