JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB309188

Human Filamin B ELISA Kit - Extracellular

Be the first to review this product! Submit a review

|

(0 Publication)

Human Filamin B ELISA Kit - Extracellular is a single-wash 90-min Simplestep used to quantify Human Filamin B with a sensitivity of 11.816 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader

View Alternative Names

FLN1L, FLN3, TABP, TAP, FLNB, Filamin-B, FLN-B, ABP-278, ABP-280 homolog, Actin-binding-like protein, Beta-filamin, Filamin homolog 1, Filamin-3, Thyroid autoantigen, Truncated actin-binding protein, Fh1, Truncated ABP

3 Images
Sandwich ELISA - Human Filamin B ELISA Kit - Extracellular (AB309188)
  • sELISA

Supplier Data

Sandwich ELISA - Human Filamin B ELISA Kit - Extracellular (AB309188)

Serum of eight individual healthy human donors was measured in duplicate. Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Filamin B concentration was determined to be 1641.5 pg/mL with a range of 857.7 – 5105.4 pg/mL.

Sandwich ELISA - Human Filamin B ELISA Kit - Extracellular (AB309188)
  • sELISA

Supplier Data

Sandwich ELISA - Human Filamin B ELISA Kit - Extracellular (AB309188)

Interpolated concentration of native Filamin B was measured in duplicate at different sample concentrations. Undiluted samples are 50% serum, plasma (EDTA), plasma (citrate), and plasma (heparin). The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

Sandwich ELISA - Human Filamin B ELISA Kit - Extracellular (AB309188)
  • sELISA

Supplier Data

Sandwich ELISA - Human Filamin B ELISA Kit - Extracellular (AB309188)

Example of human Filamin B standard curve. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Serum, EDTA Plasma, Citrate plasma, Heparin Plasma

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 11.816 pg/mL

Range

39.063 - 2500 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

style
left-column

Complementary Products

Primary Antibodies

AB282106

Anti-Filamin B antibody [EPR24525-3]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant|Trial Size

Flow Cytometry (Intracellular) - Anti-Filamin B antibody [EPR24525-3] (AB282106)

  • 0
  • 0
Proteins & Peptides

AB158452

Recombinant Human Filamin B protein (GST tag N-Terminus)

SDS-PAGE - Recombinant Human Filamin B protein (GST tag N-Terminus) (AB158452)

  • 0
  • 0
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

Product details

Human Filamin B SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Filamin B protein in human serum, plasma, saliva and cell culture supernatant. Quantitate Human Filamin B with 11.816 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

style
left-column

Precision

[ { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "6.1" }, { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.02" } ]
style
left-column

Recovery

[ { "sample": "Serum", "range": "78 - 94 %", "average": "= 85" }, { "sample": "EDTA Plasma", "range": "102 - 119 %", "average": "= 109" }, { "sample": "Citrate plasma", "range": "85 - 87 %", "average": "= 86" }, { "sample": "Heparin Plasma", "range": "89 - 103 %", "average": "= 94" } ]
style
left-column

What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab309188-1x96Tests", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB309188-CMP09", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB309188-CMP08", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB309188-CMP05", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB309188-CMP07", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB309188-CMP06", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human Filamin B Lyophilized Recombinant Protein", "number":"AB309188-CMP03", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 5BI", "number":"AB309188-CMP04", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Filamin B Capture Antibody", "number":"AB309188-CMP01", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB309188-CMP10", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Filamin B Detector Antibody", "number":"AB309188-CMP02", "productcode":"" } ] } } }
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
style
left-column
style
left-column

Product protocols

style
left-column

Target data

Connects cell membrane constituents to the actin cytoskeleton. May promote orthogonal branching of actin filaments and links actin filaments to membrane glycoproteins. Anchors various transmembrane proteins to the actin cytoskeleton. Interaction with FLNA may allow neuroblast migration from the ventricular zone into the cortical plate. Various interactions and localizations of isoforms affect myotube morphology and myogenesis. Isoform 6 accelerates muscle differentiation in vitro.
See full target information FLNB
style
left-column
style
left-column
websiteProtocolBooklet
en
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com