Human Filamin B ELISA Kit - Intracellular is a Sandwich (quantitative) ELISA kit for the measurement of Human Filamin B - Intracellular in Human in Cell Lysate, Tissue Extracts samples.
Images
Key facts
- Detection method
- Colorimetric
- Sample types
- Cell Lysate, Tissue Extracts
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 39.063 - 2500 pg/mL
- Assay time
- 1h 30m
- Sensitivity
- = 12.408 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Connects cell membrane constituents to the actin cytoskeleton. May promote orthogonal branching of actin filaments and links actin filaments to membrane glycoproteins. Anchors various transmembrane proteins to the actin cytoskeleton. Interaction with FLNA may allow neuroblast migration from the ventricular zone into the cortical plate. Various interactions and localizations of isoforms affect myotube morphology and myogenesis. Isoform 6 accelerates muscle differentiation in vitro.
Alternative names
FLN1L, FLN3, TABP, TAP, FLNB, Filamin-B, FLN-B, ABP-278, ABP-280 homolog, Actin-binding-like protein, Beta-filamin, Filamin homolog 1, Filamin-3, Thyroid autoantigen, Truncated actin-binding protein, Fh1, Truncated ABP
What's included?
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Human Filamin B ELISA Kit - Intracellular is a Sandwich (quantitative) ELISA kit for the measurement of Human Filamin B - Intracellular in Human in Cell Lysate, Tissue Extracts samples.
Key facts
- Detection method
- Colorimetric
- Sample types
- Cell Lysate, Tissue Extracts
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 39.063 - 2500 pg/mL
- Assay time
- 1h 30m
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 12.408 pg/mL
- General recovery
- 105%
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Extract | n 8 | mean - | SD - | C.V. 5.3 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Extract | n 3 | mean - | SD - | C.V. 2.67 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Cell Lysate | Average % = 105 | Range 95 - 115 % |
Sample type Tissue Extracts | Average % = 104 | Range 100 - 108 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Human Filamin B - Intracellular SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Filamin B - Intracellular protein in human cell lysate and tissue extract samples. Quantitate Human Filamin B - Intracellular with 12.408 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Product promise
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Terms & Conditions.
2 product images
Sandwich ELISA - Human Filamin B ELISA Kit - Intracellular (ab309303)
Interpolated concentration of native Filamin B was measured in duplicate at different sample concentrations. Undiluted samples are as follows: A549 cell extract 2.5 μg/mL and lung tissue extract 25 μg/mL. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.
Sandwich ELISA - Human Filamin B ELISA Kit - Intracellular (ab309303)
Example of human Filamin B standard curve. Background-subtracted data values (mean +/- SD) are graphed.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
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