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AB309393

Human FKBP51 ELISA Kit

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Human FKBP51 ELISA Kit is a Sandwich ELISA for the measurement of Human FKBP51 in Human in Cell Culture Media, Biofluids samples.

View Alternative Names

AIG6, FKBP51, FKBP5, Peptidyl-prolyl cis-trans isomerase FKBP5, PPIase FKBP5, 51 kDa FK506-binding protein, 54 kDa progesterone receptor-associated immunophilin, Androgen-regulated protein 6, FF1 antigen, FK506-binding protein 5, FKBP54, HSP90-binding immunophilin, Rotamase, 51 kDa FKBP, FKBP-51, FKBP-5, p54

1 Images
Sandwich ELISA - Human FKBP51 ELISA Kit (AB309393)
  • sELISA

Supplier Data

Sandwich ELISA - Human FKBP51 ELISA Kit (AB309393)

Example of standard curve using ab309393. This standard curve is for demonstrative purposes only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Cell culture supernatant, Plasma, Serum

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 0.2 ng/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Complementary Products

Primary Antibodies

AB126715

Anti-FKBP51 antibody [EPR6617]

RabMAb|Recombinant|KO Validated|20ul selling size

Immunocytochemistry/ Immunofluorescence - Anti-FKBP51 antibody [EPR6617] (AB126715)

  • 5
  • 2
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human FKBP51 ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human FKBP51 in serum, plasma, and cell culture supernatants. This assay employs an antibody specific for human FKBP51 coated on a 96-well plate.
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Recovery

[ { "sample": "Plasma", "range": "70 - 100 %", "average": "= 78.97" }, { "sample": "Serum", "range": "70 - 98 %", "average": "= 86.14" }, { "sample": "Cell culture media", "range": "82 - 95 %", "average": "= 87.98" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab309393-1x96Tests", "assetComponentDetails": [ { "size":"1 x 30 mL", "name":"Assay Diluent C (Item L)", "number":"AB309393-CMP06", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Lyophilized Human FKBP51 Protein Standard", "number":"AB309393-CMP11", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B (Item E)", "number":"AB309393-CMP05", "productcode":"" }, { "size":"1 x 200 µL", "name":"150X HRP-Streptavidin", "number":"AB309393-CMP12", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Human FKBP51 Antibody-coated ELISA Plate", "number":"AB309393-CMP09", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB309393-CMP08", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Human FKBP51 Detection Antibody", "number":"AB309393-CMP10", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution; 0.2M Sulfuric Acid", "number":"AB309393-CMP07", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB309393-CMP04", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

FKBP51 also known as FKBP5 is an immunophilin protein with a molecular mass around 51 kDa. It functions as a peptidyl-prolyl isomerase that assists protein folding by catalyzing the cis-trans isomerization of prolyl bonds. FKBP51 is expressed in various tissues including the brain liver kidney and skeletal muscle. The protein serves multiple mechanical roles including the modulation of protein receptor complexes and interaction with other molecular chaperones.
Biological function summary

FKBP51 plays a role in cellular stress responses and steroid receptor signaling. It is part of large complexes such as the glucocorticoid receptor complex where it modulates receptor sensitivity and translocation to the nucleus. FKBP51 influences the stress hormone pathway by affecting the receptor's binding affinity and contributing to the regulation of inflammatory responses. By playing a regulatory role in hormonal signaling FKBP51 impacts several biological processes including development and metabolism.

Pathways

FKBP51 interacts with the hypothalamus-pituitary-adrenal (HPA) axis and the AKT/mTOR signaling pathway. It interacts with proteins like Hsp90 and PHLPP impacting stress response and cellular growth regulation. The modulation of these pathways affects stress-related behaviors and cellular homeostasis. FKBP51's interaction with the HPA axis plays a significant role in stress response regulation making it an essential component of the body's adaptation mechanisms.

FKBP51 has strong associations with mood disorders and cancer. Its upregulation correlates with stress-related psychiatric conditions such as depression impacting the function of other stress-related proteins like glucocorticoid receptors. In cancer FKBP51 modulates tumor growth and survival pathways interacting with proteins involved in cell proliferation and death such as the AKT protein. Understanding FKBP51's role in these conditions helps identify potential therapeutic targets for treatment.
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Product protocols

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Target data

Immunophilin protein with PPIase and co-chaperone activities (PubMed : 11350175). Component of unligated steroid receptors heterocomplexes through interaction with heat-shock protein 90 (HSP90). Plays a role in the intracellular trafficking of heterooligomeric forms of steroid hormone receptors maintaining the complex into the cytoplasm when unliganded (PubMed : 12538866). Acts as a regulator of Akt/AKT1 activity by promoting the interaction between Akt/AKT1 and PHLPP1, thereby enhancing dephosphorylation and subsequent activation of Akt/AKT1 (PubMed : 28147277, PubMed : 28363942). Interacts with IKBKE and IKBKB which facilitates IKK complex assembly leading to increased IKBKE and IKBKB kinase activity, NF-kappa-B activation, and IFN production (PubMed : 26101251, PubMed : 31434731).
See full target information FKBP5
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websiteProtocolBooklet
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Product promise

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