Human FKBP51 ELISA Kit- Intracellular is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human FKBP51 - Intracellular in Cell Lysate, Tissue Extracts samples.
Images
Key facts
- Detection method
Colorimetric
- Sample types
Cell Lysate, Tissue Extracts
- Assay type
Sandwich (quantitative)
- Reactive species
Human
- Range
125 - 8000 pg/mL
- Assay time
1h 30m
- Sensitivity
= 21.132 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Immunophilin protein with PPIase and co-chaperone activities (PubMed:11350175). Component of unligated steroid receptors heterocomplexes through interaction with heat-shock protein 90 (HSP90). Plays a role in the intracellular trafficking of heterooligomeric forms of steroid hormone receptors maintaining the complex into the cytoplasm when unliganded (PubMed:12538866). Acts as a regulator of Akt/AKT1 activity by promoting the interaction between Akt/AKT1 and PHLPP1, thereby enhancing dephosphorylation and subsequent activation of Akt/AKT1 (PubMed:28147277, PubMed:28363942). Interacts with IKBKE and IKBKB which facilitates IKK complex assembly leading to increased IKBKE and IKBKB kinase activity, NF-kappa-B activation, and IFN production (PubMed:26101251, PubMed:31434731).
Alternative names
AIG6, FKBP51, FKBP5, Peptidyl-prolyl cis-trans isomerase FKBP5, PPIase FKBP5, 51 kDa FK506-binding protein, 54 kDa progesterone receptor-associated immunophilin, Androgen-regulated protein 6, FF1 antigen, FK506-binding protein 5, FKBP54, HSP90-binding immunophilin, Rotamase, 51 kDa FKBP, FKBP-51, FKBP-5, p54
What's included?
Human FKBP51 ELISA Kit- Intracellular is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human FKBP51 - Intracellular in Cell Lysate, Tissue Extracts samples.
Key facts
- Detection method
Colorimetric
- Sample types
Cell Lysate, Tissue Extracts
- Assay type
Sandwich (quantitative)
- Reactive species
Human
- Range
125 - 8000 pg/mL
- Assay time
1h 30m
- Assay Platform
Microplate (12 x 8 well strips)
- Sensitivity
= 21.132 pg/mL
- General recovery
103%
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Extract | n 8 | mean - | SD - | C.V. = 3.5 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Extract | n 3 | mean - | SD - | C.V. = 2.5 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Serum | Average % = 87 | Range 84 - 90 % |
Sample type Citrate plasma | Average % = 85 | Range 84 - 86 % |
Sample type EDTA Plasma | Average % = 81 | Range 80 - 82 % |
Sample type Heparin Plasma | Average % = 90 | Range 88 - 91 % |
Sample type Cell culture supernatant | Average % = 106 | Range 94 - 115 % |
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
+4°C
- Storage information
+4°C
Notes
Human FKBP51 SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of FKBP51 protein in Cell Lysate, Tissue extracts. Quantitate Human FKBP51 with 21.132 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.
Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips, available in 10-pack (10 x 96-well plates)
-Also available in a fully validated 384-well format.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
Sandwich ELISA - Human FKBP51 ELISA Kit- Intracellular (ab322377)
Interpolated concentration of native FKBP51 was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 6.25 ug/mL HepG2 cell extract. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer SSW.
Sandwich ELISA - Human FKBP51 ELISA Kit- Intracellular (ab322377)
Example of Human FKBP51 standard curve in 1X Cell Extraction Buffer SSW in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human FKBP51 ELISA Kit- Intracellular (ab322377)
Interpolated concentration of native FKBP51 was measured in duplicate at different sample concentrations. Undiluted samples are as follows: HeLa cell extract 50 ug/mL, HepG2 cell extract 12.5 ug/mL, human testes tissue extract 30 ug/mL, and human lung tissue extract 15 ug/mL. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer SSW.
Sandwich ELISA - Human FKBP51 ELISA Kit- Intracellular (ab322377)
Example of Human FKBP51 standard curve in 1X Cell Extraction Buffer SSW. Background-subtracted data values (mean +/- SD) are graphed.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
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