AB322377

Human FKBP51 ELISA Kit- Intracellular

Recombinant
SimpleStep
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Human FKBP51 ELISA Kit- Intracellular is a Sandwich (quantitative) ELISA for the measurement of Human FKBP51 - Intracellular in Human in Cell/Tissue Extracts samples.

View Alternative Names

AIG6, FKBP51, FKBP5, Peptidyl-prolyl cis-trans isomerase FKBP5, PPIase FKBP5, 51 kDa FK506-binding protein, 54 kDa progesterone receptor-associated immunophilin, Androgen-regulated protein 6, FF1 antigen, FK506-binding protein 5, FKBP54, HSP90-binding immunophilin, Rotamase, 51 kDa FKBP, FKBP-51, FKBP-5, p54

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Sandwich ELISA - Human FKBP51 ELISA Kit- Intracellular (AB322377)

sELISA

Supplier Data

Sandwich ELISA - Human FKBP51 ELISA Kit- Intracellular (AB322377)

Interpolated concentration of native FKBP51 was measured in duplicate at different sample concentrations. Undiluted samples are as follows : HeLa cell extract 50 ug/mL, HepG2 cell extract 12.5 ug/mL, human testes tissue extract 30 ug/mL, and human lung tissue extract 15 ug/mL. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer SSW.

sELISA

Supplier Data

Sandwich ELISA - Human FKBP51 ELISA Kit- Intracellular (AB322377)

Interpolated concentration of native FKBP51 was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 6.25 ug/mL HepG2 cell extract. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer SSW.

sELISA

Supplier Data

Sandwich ELISA - Human FKBP51 ELISA Kit- Intracellular (AB322377)

Example of Human FKBP51 standard curve in 1X Cell Extraction Buffer SSW in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Human FKBP51 ELISA Kit- Intracellular (AB322377)

Example of Human FKBP51 standard curve in 1X Cell Extraction Buffer SSW. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Cell Lysate, Tissue Extracts

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 21.132 pg/mL

Range

125 - 8000 pg/mL

Assay time

1h 30m

Assay Platform

Microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Human FKBP51 SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of FKBP51 protein in Cell Lysate, Tissue extracts. Quantitate Human FKBP51 with 21.132 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less

-High sensitivity, specificity and reproducibility from superior antibodies

-Fully validated in biological samples

-96-wells plate breakable into 12 x 8 wells strips, available in 10-pack (10 x 96-well plates)

-Also available in a fully validated 384-well format.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Extract", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 3.5" }, { "reproducibilityType": "Inter", "sample": "Extract", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 2.5" } ]

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Recovery

[ { "sample": "Serum", "range": "84 - 90 %", "average": "= 87" }, { "sample": "Citrate plasma", "range": "84 - 86 %", "average": "= 85" }, { "sample": "EDTA Plasma", "range": "80 - 82 %", "average": "= 81" }, { "sample": "Heparin Plasma", "range": "88 - 91 %", "average": "= 90" }, { "sample": "Cell culture supernatant", "range": "94 - 115 %", "average": "= 106" } ]

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What's included?

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Product protocols

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Target data

Immunophilin protein with PPIase and co-chaperone activities (PubMed : 11350175). Component of unligated steroid receptors heterocomplexes through interaction with heat-shock protein 90 (HSP90). Plays a role in the intracellular trafficking of heterooligomeric forms of steroid hormone receptors maintaining the complex into the cytoplasm when unliganded (PubMed : 12538866). Acts as a regulator of Akt/AKT1 activity by promoting the interaction between Akt/AKT1 and PHLPP1, thereby enhancing dephosphorylation and subsequent activation of Akt/AKT1 (PubMed : 28147277, PubMed : 28363942). Interacts with IKBKE and IKBKB which facilitates IKK complex assembly leading to increased IKBKE and IKBKB kinase activity, NF-kappa-B activation, and IFN production (PubMed : 26101251, PubMed : 31434731).

See full target information FKBP5

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websiteProtocolBooklet

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Product promise

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