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AB245715

Human FLRG ELISA Kit

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Human FLRG ELISA Kit is a single-wash 90-min Simplestep used to quantify Human FLRG with a sensitivity of 8.2 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

FLRG, UNQ674/PRO1308, FSTL3, Follistatin-related protein 3, Follistatin-like protein 3, Follistatin-related gene protein

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Sandwich ELISA - Human FLRG ELISA Kit (AB245715)
  • sELISA

Supplier Data

Sandwich ELISA - Human FLRG ELISA Kit (AB245715)

Serum from eight individual healthy human female donors was measured in duplicate.

Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean FLRG concentration was determined to be 15,897 pg/mL with a range of 13,362 - 21,062 pg/mL.

Sandwich ELISA - Human FLRG ELISA Kit (AB245715)
  • sELISA

Supplier Data

Sandwich ELISA - Human FLRG ELISA Kit (AB245715)

Example of human FLRG standard curve in Sample Diluent NS. Standard Curve comparison between human FLRG SimpleStep ELISA kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.

Sandwich ELISA - Human FLRG ELISA Kit (AB245715)
  • sELISA

Supplier Data

Sandwich ELISA - Human FLRG ELISA Kit (AB245715)

Interpolated concentrations of native FLRG in human serum and plasma samples. The concentrations of FLRG were measured in duplicates, interpolated from the FLRG standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 15%, plasma (EDTA) 15%, and plasma (heparin) 15%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean FLRG concentration was determined to be 8,804 pg/mL in serum, 8,996 pg/mL in plasma (EDTA) and 9,092 pg/mL in plasma (heparin).

Sandwich ELISA - Human FLRG ELISA Kit (AB245715)
  • sELISA

Supplier Data

Sandwich ELISA - Human FLRG ELISA Kit (AB245715)

Interpolated concentrations of native FLRG in human milk and urine samples. The concentrations of FLRG were measured in duplicates, interpolated from the FLRG standard curves and corrected for sample dilution. Undiluted samples are as follows : milk 15% and urine 15%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean FLRG concentration was determined to be 3,167 pg/mL in milk and 6,392 pg/mL in urine.

Sandwich ELISA - Human FLRG ELISA Kit (AB245715)
  • sELISA

Supplier Data

Sandwich ELISA - Human FLRG ELISA Kit (AB245715)

Interpolated concentrations of native FLRG in JAR cell supernatant samples. The concentrations of FLRG were measured in duplicates, interpolated from the FLRG standard curves and corrected for sample dilution. Undiluted samples are as follows : JAR cell supernatants 1%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean FLRG concentration was determined to be 83,631 pg/mL in JAR cell supernatant.

Sandwich ELISA - Human FLRG ELISA Kit (AB245715)
  • sELISA

Supplier Data

Sandwich ELISA - Human FLRG ELISA Kit (AB245715)

Example of human FLRG standard curve in Sample Diluent NS. The FLRG standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Urine, Heparin Plasma, Cell culture supernatant, Milk, Serum, EDTA Plasma

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 8.2 pg/mL

Range

31.25 - 2000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human FLRG ELISA Kit (ab245715) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of FLRG protein in edta plasma, hep plasma, milk, serum, urine, and cell culture supernatant. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human FLRG with 8.2 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "6.4" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.7" } ]
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Recovery

[ { "sample": "Cell culture supernatant", "range": "104 - 111 %", "average": "= 107" }, { "sample": "Milk", "range": "98 - 109 %", "average": "= 103" }, { "sample": "Serum", "range": "105 - 109 %", "average": "= 106" }, { "sample": "EDTA Plasma", "range": "102 - 106 %", "average": "= 104" }, { "sample": "Urine", "range": "94 - 109 %", "average": "= 98" }, { "sample": "Heparin Plasma", "range": "99 - 105 %", "average": "= 101" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein FSTL3 also known as Follistatin-like 3 has a molecular mass of approximately 26 kDa. Mechanically FSTL3 acts by binding and neutralizing specific members of the TGF-beta superfamily influencing cellular processes like growth differentiation and apoptosis. It is mostly expressed in tissues such as the placenta pancreas and muscle where it plays a role in various developmental and physiological events.
Biological function summary

FSTL3 serves an important function in regulating cellular dynamics by modulating activity of ligands in the TGF-beta signaling pathway. It forms a complex with activins and other growth factors to inhibit their signaling capabilities. This interaction is key in processes like managing fetal development ensuring proper organogenesis and maintaining metabolic activities.

Pathways

Extensive research places FSTL3 within the TGF-beta signaling pathway and the insulin-like growth factor (IGF) system. These pathways are critical for managing cellular growth and metabolism. FSTL3 interacts with proteins such as activins and inhibins which share roles in regulating cell proliferation and differentiation linking FSTL3 directly to these essential cellular pathways.

FSTL3 links to pathologies like type 2 diabetes and muscle wasting. FSTL3 influences insulin signaling therefore its dysregulation can contribute to insulin resistance a hallmark of type 2 diabetes. It also associates with muscle protein wasting through its interaction with activin suggesting a potential therapeutic target in conditions like cachexia.
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Product protocols

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Target data

Isoform 1 or the secreted form is a binding and antagonizing protein for members of the TGF-beta family, such as activin, BMP2 and MSTN. Inhibits activin A-, activin B-, BMP2- and MSDT-induced cellular signaling; more effective on activin A than on activin B. Involved in bone formation; inhibits osteoclast differentiation. Involved in hematopoiesis; involved in differentiation of hemopoietic progenitor cells, increases hematopoietic cell adhesion to fibronectin and seems to contribute to the adhesion of hematopoietic precursor cells to the bone marrow stroma. Isoform 2 or the nuclear form is probably involved in transcriptional regulation via interaction with MLLT10.
See full target information FSTL3
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websiteProtocolBooklet
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