Human Flt-3 ligand ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Flt-3 ligand with a sensitivity of 2 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 10x96 plates for bulk orders and 384-well plate for higher throughput
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
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Key facts
- Detection method
- Colorimetric
- Sample types
- Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 4.7 - 300 pg/mL
- Assay time
- 1h 30m
- Sensitivity
- = 2 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Stimulates the proliferation of early hematopoietic cells by activating FLT3. Synergizes well with a number of other colony stimulating factors and interleukins.
Alternative names
Fms-related tyrosine kinase 3 ligand, Flt3 ligand, Flt3L, SL cytokine, FLT3LG
What's included?
Recommended products
Human Flt-3 ligand ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Flt-3 ligand with a sensitivity of 2 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 10x96 plates for bulk orders and 384-well plate for higher throughput
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
Key facts
- Detection method
- Colorimetric
- Sample types
- Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 4.7 - 300 pg/mL
- Assay time
- 1h 30m
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 2 pg/mL
Precision
Intra assay
| Sample | n | C.V. |
|---|---|---|
Sample Serum | n 8 | C.V. 4.1 |
Inter assay
| Sample | n | C.V. |
|---|---|---|
Sample Serum | n 3 | C.V. 4.2 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Cell culture supernatant | Average % = 95 | Range 94 - 97 % |
Sample type Serum | Average % = 99 | Range 93 - 103 % |
Sample type EDTA Plasma | Average % = 94 | Range 87 - 97 % |
Sample type Heparin Plasma | Average % = 98 | Range 93 - 101 % |
Sample type Citrate plasma | Average % = 97 | Range 93 - 101 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Human Flt-3 ligand ELISA Kit (ab254507) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Flt-3 ligand protein in cell culture supernatant, cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Flt-3 ligand with 2 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Flt-3 Ligand binds to and activates Flt-3. Flt-3 Ligand works synergistically with other CSFs and interleukins to stimulate growth and differentiation of early hematopoietic cells. The predominant form of Flt-3 Ligand is the biologically active transmembrane form which is proteolytically cleaved to generate a soluble form that is also biologically active. Alternative spliced secreted soluble variant of Flt-3 Ligand has also been identified.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The Flt3 ligand (Flt3L) also known as Flt3-L Flt3 ligand protein or flt-3 ligand protein is a small cytokine with a molecular mass of approximately 18 to 19 kDa. It acts as a growth factor by binding to the Flt3 receptor tyrosine kinase on the surface of hematopoietic progenitor cells. Flt3L is expressed in various tissues including the bone marrow where it plays a significant role in hematopoiesis. It is critical in dendritic cell and B cell development impacting the immune response mechanism.
- Biological function summary
The Flt3 ligand plays an essential role in the immune system. It is a monomeric cytokine that induces proliferation of early lineage cells within the hematopoietic system. Flt3L does not function as part of a complex but acts independently to stimulate the proliferation differentiation and survival of hematopoietic cells. By interacting directly with its receptor it significantly influences the innate and adaptive immune systems through the maturation of dendritic and B cells.
- Pathways
The Flt3 ligand is involved in hematopoietic signaling pathways primarily the PI3K-Akt signaling pathway. This pathway is essential for cell survival and growth where Flt3L aids in transmitting growth signals necessary for hematopoietic cells. Flt3L also interacts with proteins such as FLT3 receptor and influences the downstream signaling cascade affecting cell fate decisions in hematopoiesis and immune cell maturation.
- Associated diseases and disorders
Flt3 ligand is connected to leukemia particularly acute myeloid leukemia (AML) due to dysregulation of the FLT3 signaling pathway. Mutations in the FLT3 receptor can lead to uncontrolled cell proliferation contributing to tumorigenesis. Additionally Flt3L plays a role in autoimmune disorders by influencing immune cell development. It is closely connected with the FLT3 receptor and other tyrosine kinases which when mutated can exacerbate disease pathology through abnormal cell signaling and proliferation.
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7 product images
Sandwich ELISA - Human Flt-3 ligand ELISA Kit (ab254507)
Human Fit-3 Ligand standard curve comparison.
Standard Curve comparison between human Fit-3 Ligand SimpleStep ELISA kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.
Sandwich ELISA - Human Flt-3 ligand ELISA Kit (ab254507)
Example of human Flt-3 Ligand standard curve in Sample Diluent NS.
The Flt-3 Ligand standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human Flt-3 ligand ELISA Kit (ab254507)
Interpolated concentrations of native Flt-3 Ligand in human serum and plasma samples.
The concentrations of Flt-3 Ligand were measured in duplicates, interpolated from the Flt-3 Ligand standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 50%, plasma (citrate) 50%, plasma (heparin) 50%, and plasma (EDTA) 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Flt-3 Ligand concentration was determined to be 65.35 pg/mL in neat serum, 57.01 pg/mL in neat plasma (citrate), 54.11 pg/mL in neat plasma (heparin), and 64.57 pg/mL in neat plasma (EDTA).
Sandwich ELISA - Human Flt-3 ligand ELISA Kit (ab254507)
Interpolated concentrations of native Flt-3 Ligand in human PBMC cell culture supernatant samples. Human PBMC were cultured in the presence (stimulated) or absence (unstimulated) of 1.5% PHA-M.
Human peripheral blood mononuclear cells (PBMC) were cultured in the presence (stimulated) or absence (unstimulated) of 1.5% PHA-M for 36 hours. The concentrations of Flt-3 Ligand were measured in duplicates, interpolated from the Flt-3 Ligand standard curves and corrected for sample dilution. Undiluted samples are as follows: stimulated PBMC supernatant 100% and unstimulated PBMC supernatant 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Flt-3 Ligand concentration was determined to be 22.72 pg/mL in neat PHA-M stimulated PBMC cell culture supernatant, 3.742 pg/mL in neat unstimulated PBMC cell culture supernatant, and undetectable in naïve cell culture media (data not shown).
Sandwich ELISA - Human Flt-3 ligand ELISA Kit (ab254507)
Serum from ten individual healthy human female donors was measured in duplicate.
Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Flt-3 Ligand concentration was determined to be 63.87 pg/mL with a range of 26.63 – 105.1 pg/mL.
Sandwich ELISA - Human Flt-3 ligand ELISA Kit (ab254507)
Example of human Flt-3 Ligand standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human Flt-3 ligand ELISA Kit (ab254507)
Interpolated concentration of native Flt-3 Ligand was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 50% human serum. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.
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