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AB327301

Human FLT-3 Ligand ELISA Kit, Chemiluminescent

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Human FLT-3 Ligand ELISA Kit, Chemiluminescent is a 55-min single-wash SimpleStep Ignite™ ELISA for the rapid quantification of Human FLT-3 Ligand with higher sensitivity and broader measuring range.

- Single 30 min incubation and single-wash step for results in less than an hour.
- Chemiluminescent detection allows a broad dynamic range: 0.37 - 800 pg/ml with a sensitivity of 0.13 pg/mL.
- Different formats for different needs: 10x 96-well plates for bulk orders
- Design your own immunoassay: we also offer the conjugation-ready antibody pair.
- Patent pending
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Sandwich ELISA - Human FLT-3 Ligand ELISA Kit, Chemiluminescent (AB327301)
  • sELISA

Supplier Data

Sandwich ELISA - Human FLT-3 Ligand ELISA Kit, Chemiluminescent (AB327301)

Serum from ten individual healthy human female donors was measured in duplicate.

Interpolated dilution factor corrected values are plotted (mean +/- SD n=2). The mean Flt-3 Ligand concentration was determined to be 63.87 pg/mL with a range of 26.63 – 105.1 pg/mL.

Sandwich ELISA - Human FLT-3 Ligand ELISA Kit, Chemiluminescent (AB327301)
  • sELISA

Supplier Data

Sandwich ELISA - Human FLT-3 Ligand ELISA Kit, Chemiluminescent (AB327301)

Interpolated concentrations of native Flt-3 Ligand in human PBMC cell culture supernatant samples. Human PBMC were cultured in the presence (stimulated) or absence (unstimulated) of 1.5% PHA-M.

Human peripheral blood mononuclear cells (PBMC) were cultured in the presence (stimulated) or absence (unstimulated) of 1.5% PHA-M for 36 hours. The concentrations of Flt-3 Ligand were measured in duplicates interpolated from the Flt-3 Ligand standard curves and corrected for sample dilution. Undiluted samples are as follows : stimulated PBMC supernatant 100% and unstimulated PBMC supernatant 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD n=2). The mean Flt-3 Ligand concentration was determined to be 22.72 pg/mL in neat PHA-M stimulated PBMC cell culture supernatant 3.742 pg/mL in neat unstimulated PBMC cell culture supernatant and undetectable in naive cell culture media (data not shown).

Sandwich ELISA - Human FLT-3 Ligand ELISA Kit, Chemiluminescent (AB327301)
  • sELISA

Supplier Data

Sandwich ELISA - Human FLT-3 Ligand ELISA Kit, Chemiluminescent (AB327301)

Interpolated concentrations of native Flt-3 Ligand in human serum and plasma samples.

The concentrations of Flt-3 Ligand were measured in duplicates interpolated from the Flt-3 Ligand standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 50% plasma (citrate) 50% plasma (heparin) 50% and plasma (EDTA) 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD n=2). The mean Flt-3 Ligand concentration was determined to be 65.35 pg/mL in neat serum 57.01 pg/mL in neat plasma (citrate) 54.11 pg/mL in neat plasma (heparin) and 64.57 pg/mL in neat plasma (EDTA).

Sandwich ELISA - Human FLT-3 Ligand ELISA Kit, Chemiluminescent (AB327301)
  • sELISA

Lab

Sandwich ELISA - Human FLT-3 Ligand ELISA Kit, Chemiluminescent (AB327301)

Standard curve comparison between the Chemiluminescent Human Flt-3 Ligand SimpleStep ELISA and colorimetric Human Flt-3 Ligand SimpleStep ELISA (ab254507) in Sample Diluent NS. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human FLT-3 Ligand ELISA Kit, Chemiluminescent (AB327301)
  • sELISA

Lab

Sandwich ELISA - Human FLT-3 Ligand ELISA Kit, Chemiluminescent (AB327301)

Dynamic range comparison between the Chemiluminescent Human Flt-3 Ligand SimpleStep ELISA, colorimetric Human Flt-3 Ligand SimpleStep ELISA (ab254507), and leading colorimetric competitor. The Chemiluminescent SimpleStep ELISA kit shows increased sensitivity and increased dynamic range.

Key facts

Detection method

Luminescent

Sample types

Cell culture supernatant, Serum, EDTA Plasma, Heparin Plasma, Citrate plasma

Reacts with

Human, Monkey

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 0.13 pg/mL

Range

0.37 - 800 pg/mL

Assay time

55m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human FLT-3 Ligand ELISA Kit, Chemiluminescent (ab327301) is a rapid single-wash sandwich 55-min ELISA to measure Human FLT-3 Ligand in Cell culture supernatant, Serum, EDTA Plasma, Heparin Plasma, Citrate plasma with a sensitivity of 0.13 pg/mL.

How the assay works

Human FLT-3 Ligand ELISA Kit, Chemiluminescent (ab327301) SimpleStep Ignite™ ELISA employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step during a 30 min only incubation, significantly reducing assay time. By utilizing a chemiluminscent detection method this improves the sensitivity and dynamic range when compared to traditional colorimetric ELISAs.

Assay Specificity

Our SimpleStep Ignite™ ELISA kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human FLT-3 Ligand (ab327301) protocol summary

1. Mix : add samples / standards to the wells together with the capture and detector antibody cocktail. Incubate 30 min at room temperature
2. Wash
3. Add ChemiHRP development solution - incubate for 2 min
4. Read the results on a plate reader capable of luminescence detection

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-Flt-3 Ligand antibody [EPR22288-52] - BSA and Aside free (Capture) ab259725
- Anti-Flt-3 Ligand antibody [EPR22288-201] - BSA and Aside free (Detector) ab259726
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Precision

[ { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.1" }, { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.2" } ]
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Recovery

[ { "sample": "Cell culture supernatant", "range": "94 - 97 %", "average": "= 95" }, { "sample": "Serum", "range": "93 - 103 %", "average": "= 99" }, { "sample": "EDTA Plasma", "range": "87 - 97 %", "average": "= 94" }, { "sample": "Heparin Plasma", "range": "93 - 101 %", "average": "= 98" }, { "sample": "Citrate plasma", "range": "93 - 101 %", "average": "= 97" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

Stimulates the proliferation of early hematopoietic cells by activating FLT3. Synergizes well with a number of other colony stimulating factors and interleukins. Required for the development of B cells, and dendritic cells (DCs).
See full target information FLT3LG

Alternative Names

Fms-related tyrosine kinase 3 ligand, Flt3 ligand, Flt3L, SL cytokine, FLT3LG
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websiteProtocolBooklet
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com