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AB213781

Human Folate Binding Protein / FBP ELISA Kit

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Human Folate Binding Protein / FBP ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human Folate Binding Protein / FBP in Human in Cell Culture Media, Biofluids samples.

View Alternative Names

FOLR, FOLR1, Folate receptor alpha, FR-alpha, Adult folate-binding protein, Folate receptor 1, KB cells FBP, Ovarian tumor-associated antigen MOv18, FBP

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Sandwich ELISA - Human Folate Binding Protein / FBP ELISA Kit (AB213781)
  • sELISA

Supplier Data

Sandwich ELISA - Human Folate Binding Protein / FBP ELISA Kit (AB213781)

Human Folate Binding Protein / FBP ELISA Kit (ab213781) Standard Curve.

Key facts

Detection method

Colorimetric

Sample types

Cell culture supernatant, EDTA Plasma, Heparin Plasma, Milk, Saliva, Serum, Urine

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

< 10 pg/mL

Range

46.9 - 3000 pg/mL

Assay time

3h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

The Human Folate Binding Protein / FBP Enzyme-Linked Immunosorbent Assay (ELISA) kit (ab213781) is designed for the quantitative measurement of Human Folate Binding Protein / FBP in cell culture supernatants, serum, plasma (heparin, EDTA), saliva, urine and Human milk.

The ELISA kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Folate Binding Protein / FBP has been pre-coated onto 96-well plates. Standards (Expression system for standard: E.coli; Immunogen sequence: R25-S234) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Folate Binding Protein / FBP is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB is used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human Folate Binding Protein / FBP amount of sample captured in plate.

Folate Binding Protein / FBP adult is a protein that in humans is encoded by the Folate Binding Protein / FBP gene. The protein encoded by this gene is a member of the folate receptor (FOLR) family. It is mapped to 11q13.4. Folate Binding Protein / FBP expression in Jurkat cells facilitated MBG or EBO entry, and FR-blocking reagents inhibited infection of MBG or EBO. This gene product is a secreted protein that either anchors to membranes via a glycosyl-phosphatidylinositol linkage or exists in a soluble form. The Folate Binding Protein / FBP gene encodes the adult folate receptor, or folate-binding protein (FBP), which has a high affinity for folic acid and for several reduced folic acid derivatives, and mediates delivery of 5-methyltetrahydrofolate to the interior of cells. Folate Binding Protein / FBP is also an important regulator of milk protein synthesis.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Sample 1", "replicates": 24, "mean": "108 pg/mL", "standardDeviation": "8.53", "coefficientOfVariability": "7.9" }, { "reproducibilityType": "Inter", "sample": "Sample 2", "replicates": 24, "mean": "312 pg/mL", "standardDeviation": "24.96", "coefficientOfVariability": "8" }, { "reproducibilityType": "Inter", "sample": "Sample 3", "replicates": 24, "mean": "1388 pg/mL", "standardDeviation": "119.36", "coefficientOfVariability": "8.6" }, { "reproducibilityType": "Intra", "sample": "Sample 1", "replicates": 16, "mean": "103 pg/mL", "standardDeviation": "7.41", "coefficientOfVariability": "7.2" }, { "reproducibilityType": "Intra", "sample": "Sample 2", "replicates": 16, "mean": "333 pg/mL", "standardDeviation": "22.31", "coefficientOfVariability": "6.7" }, { "reproducibilityType": "Intra", "sample": "Sample 3", "replicates": 16, "mean": "1306 pg/mL", "standardDeviation": "88.8", "coefficientOfVariability": "6.8" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Folate Binding Protein (FBP) also known as FolR1 protein or FOLR-1 is a significant protein with a mass of about 32-38 kDa. It binds folate with high affinity and facilitates its transport into the cell. FBP can be found in various tissues with notable expression in the placenta kidney and certain epithelial tissues. The protein mediates folate uptake by capturing folates in the extracellular matrix and channeling them through receptor-mediated endocytosis.
Biological function summary

FBP plays a critical role in cellular growth and replication by ensuring sufficient folate supply. It belongs to a family of receptors not forming part of a larger protein complex. FBP's primary function is delivering folate an important B vitamin which acts as a one-carbon donor in DNA synthesis and repair. Optimal folate transport by FBP supports cellular functions particularly in rapidly dividing cells.

Pathways

FBP significantly contributes to the folate-mediated one-carbon metabolic pathway critical for nucleotide biosynthesis. This pathway interconnects with the homocysteine remethylation cycle. FBP associates with other enzymes such as dihydrofolate reductase (DHFR) and methylenetetrahydrofolate reductase (MTHFR) which also play key roles in these pathways. These interactions ensure efficient folate metabolism and maintenance of normal cellular functions.

FBP's relation to cancer and neural tube defects is well documented. Overexpression of FBP has been observed in various cancers including ovarian and breast cancer which indicates its potential role in tumor progression. In neural tube defects altered folate transport due to variations in FBP expression impacts neural development. FBP connects to other markers like HER2 in cancer which may form part of a therapeutic target strategy.
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Product protocols

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Target data

Binds to folate and reduced folic acid derivatives and mediates delivery of 5-methyltetrahydrofolate and folate analogs into the interior of cells (PubMed : 19074442, PubMed : 23851396, PubMed : 23934049, PubMed : 2527252, PubMed : 8033114, PubMed : 8567728). Has high affinity for folate and folic acid analogs at neutral pH (PubMed : 23851396, PubMed : 23934049, PubMed : 2527252, PubMed : 8033114, PubMed : 8567728). Exposure to slightly acidic pH after receptor endocytosis triggers a conformation change that strongly reduces its affinity for folates and mediates their release (PubMed : 8567728). Required for normal embryonic development and normal cell proliferation (By similarity).
See full target information FOLR1
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websiteProtocolBooklet
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