AB277710

Human Folate Binding Protein (FBP) ELISA Kit

Recombinant
SimpleStep
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Human Folate Binding Protein (FBP) ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Folate Binding Protein (FBP) with a sensitivity of 3.08 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

FOLR, FOLR1, Folate receptor alpha, FR-alpha, Adult folate-binding protein, Folate receptor 1, KB cells FBP, Ovarian tumor-associated antigen MOv18, FBP

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Sandwich ELISA - Human Folate Binding Protein (FBP) ELISA Kit (AB277710)

sELISA

Supplier Data

Sandwich ELISA - Human Folate Binding Protein (FBP) ELISA Kit (AB277710)

Serum from ten individual healthy human female donors was measured in duplicate.

Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Folate Binding Protein concentration was determined to be 501.99 ng/mL with a range of 296.15 – 775.05 pg/mL.

sELISA

Supplier Data

Sandwich ELISA - Human Folate Binding Protein (FBP) ELISA Kit (AB277710)

Interpolated concentrations of native Folate Binding Protein in human cell culture supernatant samples.

The concentrations of Folate Binding Protein were measured in duplicates, interpolated from the Folate Binding Protein standard curves and corrected for sample dilution. Undiluted samples are as follows : HeLa supernatant 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Folate Binding Protein concentration was determined to be 151.87 pg/mL in HeLa supernatant.

sELISA

Supplier Data

Sandwich ELISA - Human Folate Binding Protein (FBP) ELISA Kit (AB277710)

Interpolated concentrations of native Folate Binding Protein in human urine, saliva, and milk samples.

The concentrations of Folate Binding Protein were measured in duplicates, interpolated from the Folate Binding Protein standard curves and corrected for sample dilution. Undiluted samples are as follows : urine 1 : 50, saliva 1 : 50, and milk 1 : 800. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Folate Binding Protein concentration was determined to be 12.17 ng/ml in urine, 20.41 ng/ml in saliva, and 499.18 ng/ml in milk.

sELISA

Supplier Data

Sandwich ELISA - Human Folate Binding Protein (FBP) ELISA Kit (AB277710)

Interpolated concentrations of native Folate Binding Protein in human serum and plasma samples.

The concentrations of Folate Binding Protein were measured in duplicates, interpolated from the Folate Binding Protein standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 100%, plasma (citrate) 100%, plasma (heparin) 100% and plasma (EDTA) 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Folate Binding Protein concentration was determined to be 504.27 pg/mL in serum, 294.18 pg/mL in plasma (citrate), 355.36 pg/mL in plasma (heparin), and 290.02 pg/mL in plasma (EDTA).

sELISA

Unknown

Sandwich ELISA - Human Folate Binding Protein (FBP) ELISA Kit (AB277710)

Example of human Folate Binding Protein standard curve in Sample Diluent NS.

The Folate Binding Protein standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Saliva, Urine, Heparin Plasma, Citrate plasma, Cell culture supernatant, Milk, Serum, EDTA Plasma

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 3.08 pg/mL

Range

10.16 - 650 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Human Folate Binding Protein (FBP) ELISA kit (ab277710) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human Folate Binding Protein (FBP) protein in human serum, plasma, cell culture supernatant, urine, salive, and milk samples. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Folate Binding Protein (FBP) with 3.08 pg/mL sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

Folate Binding Protein is a receptor involved in update of folic acid and reduced folates. FBP is primarily expressed on epithelial cells and is essential during early embryogenesis. Upregulation of FBP is seen in some cancers and can be a marker for ovarian cancer.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.6" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.6" } ]

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Recovery

[ { "sample": "Cell culture supernatant", "range": "101 - 109 %", "average": "= 104" }, { "sample": "Milk", "range": "96 - 110 %", "average": "= 105" }, { "sample": "Serum", "range": "85 - 96 %", "average": "= 91" }, { "sample": "EDTA Plasma", "range": "94 - 108 %", "average": "= 102" }, { "sample": "Urine", "range": "95 - 109 %", "average": "= 103" }, { "sample": "Saliva", "range": "95 - 103 %", "average": "= 99" }, { "sample": "Heparin Plasma", "range": "89 - 104 %", "average": "= 98" }, { "sample": "Citrate plasma", "range": "97 - 109 %", "average": "= 104" } ]

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What's included?

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Folate Binding Protein (FBP) also known as FolR1 protein or FOLR-1 is a significant protein with a mass of about 32-38 kDa. It binds folate with high affinity and facilitates its transport into the cell. FBP can be found in various tissues with notable expression in the placenta kidney and certain epithelial tissues. The protein mediates folate uptake by capturing folates in the extracellular matrix and channeling them through receptor-mediated endocytosis.

Biological function summary

FBP plays a critical role in cellular growth and replication by ensuring sufficient folate supply. It belongs to a family of receptors not forming part of a larger protein complex. FBP's primary function is delivering folate an important B vitamin which acts as a one-carbon donor in DNA synthesis and repair. Optimal folate transport by FBP supports cellular functions particularly in rapidly dividing cells.

Pathways

FBP significantly contributes to the folate-mediated one-carbon metabolic pathway critical for nucleotide biosynthesis. This pathway interconnects with the homocysteine remethylation cycle. FBP associates with other enzymes such as dihydrofolate reductase (DHFR) and methylenetetrahydrofolate reductase (MTHFR) which also play key roles in these pathways. These interactions ensure efficient folate metabolism and maintenance of normal cellular functions.

FBP's relation to cancer and neural tube defects is well documented. Overexpression of FBP has been observed in various cancers including ovarian and breast cancer which indicates its potential role in tumor progression. In neural tube defects altered folate transport due to variations in FBP expression impacts neural development. FBP connects to other markers like HER2 in cancer which may form part of a therapeutic target strategy.

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Product protocols

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Target data

Binds to folate and reduced folic acid derivatives and mediates delivery of 5-methyltetrahydrofolate and folate analogs into the interior of cells (PubMed : 19074442, PubMed : 23851396, PubMed : 23934049, PubMed : 2527252, PubMed : 8033114, PubMed : 8567728). Has high affinity for folate and folic acid analogs at neutral pH (PubMed : 23851396, PubMed : 23934049, PubMed : 2527252, PubMed : 8033114, PubMed : 8567728). Exposure to slightly acidic pH after receptor endocytosis triggers a conformation change that strongly reduces its affinity for folates and mediates their release (PubMed : 8567728). Required for normal embryonic development and normal cell proliferation (By similarity).

See full target information FOLR1

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websiteProtocolBooklet

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Human Folate Binding Protein (FBP) ELISA Kit

Sandwich ELISA - Human Folate Binding Protein (FBP) ELISA Kit (AB277710)

Sandwich ELISA - Human Folate Binding Protein (FBP) ELISA Kit (AB277710)

Sandwich ELISA - Human Folate Binding Protein (FBP) ELISA Kit (AB277710)

Sandwich ELISA - Human Folate Binding Protein (FBP) ELISA Kit (AB277710)

Sandwich ELISA - Human Folate Binding Protein (FBP) ELISA Kit (AB277710)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay time

Assay Platform

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

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Product promise