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AB192147

Human Follistatin ELISA Kit

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(1 Publication)

Human Follistatin ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Follistatin with a sensitivity of 28 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader

View Alternative Names

Follistatin, FS, Activin-binding protein, FST

5 Images
ELISA - Human Follistatin ELISA Kit (AB192147)
  • ELISA

Supplier Data

ELISA - Human Follistatin ELISA Kit (AB192147)
Sandwich ELISA - Human Follistatin ELISA Kit (AB192147)
  • sELISA

Supplier Data

Sandwich ELISA - Human Follistatin ELISA Kit (AB192147)

Interpolated values of Follistatin from HepG2 cell culture supernatants diluted within the working range of the assay (+/- SD, n =2)

Sandwich ELISA - Human Follistatin ELISA Kit (AB192147)
  • sELISA

Supplier Data

Sandwich ELISA - Human Follistatin ELISA Kit (AB192147)

Interpolated values of Follistatin from a titration of Normal Human Serum (NHS) and Normal Human Plasma (EDTA) diluted within the working range of the assay and spiked with recombinant follistatin.

(mean +/- SD, n =2).

Sandwich ELISA - Human Follistatin ELISA Kit (AB192147)
  • sELISA

Supplier Data

Sandwich ELISA - Human Follistatin ELISA Kit (AB192147)

Quantitation of Follistatin expression in serum samples diluted to within working range from individual donors.

Interpolated values of Follistatin are plotted on the bar graph. No signal was detected in Donors 6, 7 or 8.

Sandwich ELISA - Human Follistatin ELISA Kit (AB192147)
  • sELISA

Supplier Data

Sandwich ELISA - Human Follistatin ELISA Kit (AB192147)

Example of Follistatin standard curve.

Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Cell culture supernatant, Serum, EDTA Plasma

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 28 pg/mL

Range

0.08 - 5 ng/mL

Assay time

1h 30m

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human Follistatin ELISA Kit ab192147 is a rapid single-wash 90-min Sandwich ELISA to measure Human Follistatin in cell culture supernatant, EDTA plasma, serum. This SimpleStep sensitivity is 28 pg/mL.

How the assay works

Human Follistatin SimpleStep ELISA®employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human Follistatin ELISA Kit ab192147 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

Follistatin is a single-chain polypeptide consisting of about 344 amino acids. The follistatin protein is encoded by a relatively small gene size of 6 kb and 6 exons with alternative splice variants and post translational modifications to give a variety of molecular weights between 31 – 39 kDa. Follistatin forms a high affinity complex with activin sequestering it from its receptor thus having an inhibitory effect. Follistatin acts to promote muscle growth in mammals by acting as an antagonist to the protein myostatin. Myostatin acts as an inhibitor to muscle growth.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.5" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "6.3" } ]
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Recovery

[ { "sample": "Serum", "range": "99 - 125 %", "average": "= 110" }, { "sample": "EDTA Plasma", "range": "89 - 95 %", "average": "= 92" }, { "sample": "Cell culture media", "range": "85 - 86 %", "average": "= 86" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab192147-1x96Tests", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB192147-CMP07", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB192147-CMP11", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB192147-CMP08", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB192147-CMP06", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB192147-CMP09", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB192147-CMP10", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human Follistatin Lyophilized Recombinant Protein", "number":"AB192147-CMP05", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 5BI", "number":"AB192147-CMP04", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Follistatin Capture Antibody", "number":"AB192147-CMP01", "productcode":"" }, { "size":"1 x 20 mL", "name":"Sample Diluent 25BP", "number":"AB192147-CMP03", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Follistatin Detector Antibody", "number":"AB192147-CMP02", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Follistatin also known as FS or FST is a glycoprotein with an approximate mass of 32 kDa. This protein is part of the follistatin family and plays an important role in various physiological mechanisms. Follistatin synthesizes and secretes in different tissues including the liver gonads and skeletal muscles. It binds to and inhibits members of the transforming growth factor-beta (TGF-?) superfamily particularly activin. This binding prevents activin from interacting with its receptors.
Biological function summary

Follistatin modulates multiple processes such as cell growth differentiation and reproductive physiology. It acts as a binding protein for activin neutralizing its activity. By preventing activin from engaging with its receptor complexes follistatin influences developmental and regenerative processes. Despite not being a part of a transmembrane complex its interaction with activin impacts extracellular interactions.

Pathways

Follistatin plays a significant role in the regulation of the TGF-? signaling pathway and the muscle growth pathway. In the TGF-? pathway it counteracts the signaling of activin which influences cellular homeostasis and inflammatory responses. In the muscle growth pathway follistatin interferes with myostatin another member of the TGF-? family promoting muscle growth. This cross-talk with proteins such as myostatin demonstrates follistatin's influence in muscle regeneration and development.

Follistatin has clear associations with muscle-related and reproductive disorders. It is linked to muscle wasting diseases such as muscular dystrophy due to its inhibitory role on myostatin known to restrain muscle growth. Follistatin abnormalities can also contribute to conditions like polycystic ovary syndrome (PCOS) due to its regulation of reproductive processes. These ties display follistatin's vital interactions with proteins like activin and myostatin highlighting its importance in disease pathways.
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Product protocols

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Target data

Multifunctional regulatory protein whose primary function is to antagonize members of the transforming growth factor beta (TGF-beta) superfamily including activin, myostatin, GDF11 or bone morphogenetic proteins (BMPs) (PubMed : 11279126, PubMed : 16482217, PubMed : 18535106). Mechanistically, binds to these ligands in the extracellular space, blocking their type II receptor-binding site to inhibit downstream signaling (PubMed : 16482217). Plays an essential role in muscle fiber formation and growth both by preventing the repressive effects of myostatin and through SMAD3/AKT/mTOR signaling independently of myostatin (By similarity). Also promotes neural differentiation by antagonizing the action BMP4 (By similarity). Acts as a specific inhibitor of the biosynthesis and secretion of pituitary follicle stimulating hormone (FSH) by sequestering activin A/INHBA (PubMed : 11279126). On the other hand, translocates into the nucleus where it down-regulates rRNA synthesis and ribosome biogenesis to maintain cellular energy homeostasis by binding to rDNA.
See full target information FST
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

EMBO molecular medicine 12:e10895 PubMed32134197

2020

Follistatin is a novel therapeutic target and biomarker in FLT3/ITD acute myeloid leukemia.

Applications

Unspecified application

Species

Unspecified reactive species

Bai-Liang He,Ning Yang,Cheuk Him Man,Nelson Ka-Lam Ng,Chae-Yin Cher,Ho-Ching Leung,Leo Lai-Hok Kan,Bowie Yik-Ling Cheng,Stephen Sze-Yuen Lam,Michelle Lu-Lu Wang,Chun-Xiao Zhang,Hin Kwok,Grace Cheng,Rakesh Sharma,Alvin Chun-Hang Ma,Chi Wai Eric So,Yok-Lam Kwong,Anskar Yu-Hung Leung
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websiteProtocolBooklet
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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