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AB267621

Human FOLR2 ELISA Kit

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Human FOLR2 ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human FOLR2 in Human in Cell Culture Media, Biofluids samples.

View Alternative Names

Folate receptor beta, FR-beta, Folate receptor 2, Placental folate-binding protein, FBP, FOLR2

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Sandwich ELISA - Human FOLR2 ELISA Kit (AB267621)
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Supplier Data

Sandwich ELISA - Human FOLR2 ELISA Kit (AB267621)

This standard curve is for demonstration only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Cell culture supernatant, Citrate plasma, Heparin Plasma, Serum

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 0.17 ng/mL

Range

0.156 - 10 ng/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human FOLR2 ELISA Kit is designed for the quantitative determination of FOLR2 in cell culture supernatants, plasma and serum samples. This assay employs an antibody specific for human FOLR2 coated on a 96-well plate. Standards and samples are pipetted into the wells and FOLR2 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human FOLR2 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of FOLR2 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Δ Note: Collect plasma using citrate and heparin as an anticoagulant. EDTA is not recommended as anticoagulants for use in this assay.

This ELISA kit shows no cross-reactivity with any of the cytokines tested: human ALK-1, B7- H2, BLAME, BMP-8, CD28, Common beta Chain (CD131), Contactin-1, Desmoglein-1, Desmoglein-3, EDAR, EphA1, EphB6, Ephrin-B3, Epiregulin, FGF-12, FGF-17, Galectin-8, Glypican 1, Glypican 5, GHR, IFNgamma R1, IL-22 R alpha 1, IL-22BP, IL-23 R, IL-31 RA, IL7 R alpha, Integrin alpha 5, MDM2, Nectin-1, NKp30, Nogo Receptor, Notch-3, OSM R beta, Prolactin R, RELT, Ryk, Semaphorin 6D, Semaphorin 7A, Siglec-11.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 12" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" } ]
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Recovery

[ { "sample": "Cell culture supernatant", "range": "81 - 120 %", "average": "= 103.7" }, { "sample": "Serum", "range": "84 - 134 %", "average": "= 110.8" }, { "sample": "Plasma", "range": "71 - 76 %", "average": "= 74.2" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab267621-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB267621-CMP07", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB267621-CMP05", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Substrate Solution", "number":"AB267621-CMP06", "productcode":"" }, { "size":"1 x 200 µL", "name":"150X HRP-Streptavidin Concentrate", "number":"AB267621-CMP01", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Anti-Human FOLR2 Detection Antibody", "number":"AB267621-CMP08", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B", "number":"AB267621-CMP03", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Anti-Human FOLR2 coated Microplate (12 x 8 wells)", "number":"AB267621-CMP09", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human FOLR2 Standard (Lyophilized)", "number":"AB267621-CMP02", "productcode":"" }, { "size":"1 x 30 mL", "name":"Assay Diluent C", "number":"AB267621-CMP04", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Folate receptor beta also known as FOLR2 is a protein with a molecular mass of approximately 32 kDa. It facilitates the transport of folate into cells. FOLR2 binds folate with high affinity and plays an important role in cellular metabolism. The receptor is mainly expressed in the placenta and certain myeloid cells such as macrophages and monocytes. Its expression is also notable in certain cancer cells but not in most normal tissues.
Biological function summary

Folate receptor beta plays a significant role in folate uptake contributing to DNA synthesis and repair processes. FOLR2 as a part of the cell membrane forms a complex with other molecules to capture and internalize folate from the extracellular environment. This process is essential for the proliferation and survival of cells that require large amounts of folate including developing embryos and certain immune cells.

Pathways

The function of folate receptor beta connects with the folate-mediated one-carbon metabolism pathway critical for nucleotide biosynthesis and methylation reactions. This pathway influences the synthesis of purines and pyrimidines supporting DNA replication and repair. FOLR2 interacts with folate transport proteins and enzymes involved in the methionine cycle which interlinks with amino acid metabolism and epigenetic regulation.

Folate receptor beta is associated with rheumatoid arthritis and ovarian cancer. In rheumatoid arthritis FOLR2 expression on activated macrophages indicates its potential as a biomarker and target for therapy. The overexpression of FOLR2 in ovarian cancer makes it a target for folate-linked therapeutic agents. The relationship between FOLR2 and immune proteins in inflammatory conditions highlights its role in modulating immune responses in disease contexts.
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Product protocols

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Target data

Binds to folate and reduced folic acid derivatives and mediates delivery of 5-methyltetrahydrofolate and folate analogs into the interior of cells. Has high affinity for folate and folic acid analogs at neutral pH. Exposure to slightly acidic pH after receptor endocytosis triggers a conformation change that strongly reduces its affinity for folates and mediates their release.
See full target information FOLR2
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websiteProtocolBooklet
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