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AB267613

Human FOLR3 ELISA Kit

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(1 Publication)

Human FOLR3 ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human FOLR3 in Human in Biofluids, Cell Culture Media samples.

View Alternative Names

Folate receptor gamma, FR-gamma, Folate receptor 3, FOLR3

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Sandwich ELISA - Human FOLR3 ELISA Kit (AB267613)
  • sELISA

Supplier Data

Sandwich ELISA - Human FOLR3 ELISA Kit (AB267613)

Human FOLR3 ELISA kit (ab267613) Standard curve.

Data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture media, Serum

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 32 pg/mL

Range

32.77 - 8000 pg/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human FOLR3 ELISA Kit is designed for the quantitative determination of FOLR3 in cell culture supernatants, plasma and serum samples.

This assay employs an antibody specific for human FOLR3 coated on a 96-well plate. Standards and samples are pipetted into the wells and FOLR3 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human FOLR3 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of FOLR3 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" }, { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 12" } ]
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Recovery

[ { "sample": "Serum", "range": "96 - 110 %", "average": "= 103" }, { "sample": "Plasma", "range": "86 - 108 %", "average": "= 96" }, { "sample": "Cell culture media", "range": "89 - 95 %", "average": "= 91" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab267613-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB267613-CMP06", "productcode":"" }, { "size":"1 x 200 µL", "name":"100X HRP-Streptavidin Concentrate", "number":"AB267613-CMP05", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB267613-CMP03", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Substrate Solution", "number":"AB267613-CMP04", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Anti-Human FOLR3 coated Microplate (12 x 8 wells)", "number":"AB267613-CMP08", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Anti-Human FOLR3 Detection Antibody", "number":"AB267613-CMP07", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent", "number":"AB267613-CMP02", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human FOLR3 Standard (Lyophilized)", "number":"AB267613-CMP01", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

FOLR3 also known as folate receptor gamma is a member of the folate receptor family responsible for binding and transporting folate across cell membranes. This protein has a molecular mass of about 32 kDa. FOLR3 is primarily found in certain tissues including the placenta and liver and it exhibits limited expression in several epithelial tissues. Folate receptors including FOLR3 function by binding folates and delivering them into cells via endocytosis a process essential for the maintenance of regular cellular activity and function.
Biological function summary

Folate receptor gamma plays a significant role in the transport and uptake of folate an important vitamin necessary for DNA synthesis and methylation. The receptor assists in various cellular processes including nucleotide biosynthesis. FOLR3 works as part of a larger complex of folate receptors which ensure cells have adequate folate levels for normal metabolic functions. By doing so it helps facilitate normal cell growth and division critical in rapidly dividing cells.

Pathways

FOLR3 is notably involved in the folate metabolism pathway contributing to DNA synthesis and repair processes. This pathway connects with the one-carbon metabolism pathway where folate is an essential donor of carbons necessary for methylation reactions. In these pathways FOLR3 interacts with other folate receptors like FOLR1 and FOLR2 to maintain efficient folate transport and cellular homeostasis. Ensuring proper folate availability supports homocysteine metabolism which is another critical aspect of these pathways.

FOLR3 relates notably to conditions such as cancer and inflammatory disorders. Cancer cells often exhibit elevated folate receptor expression including FOLR3 due to their increased requirement for folate in rapid cell proliferation. Inflammatory disorders also see changes in folate receptor expression which might affect immune cell functions and inflammation regulation. In these contexts FOLR3 interacts with tumor-associated proteins and immune modulators highlighting its potential as a therapeutic target in medical research and treatment development.
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Product protocols

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Target data

Binds to folate and reduced folic acid derivatives and mediates delivery of 5-methyltetrahydrofolate to the interior of cells. Isoform Short does not bind folate.
See full target information Folate receptor gamma
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 23: PubMed36361563

2022

iTRAQ Proteomics Identified the Potential Biomarkers of Coronary Artery Lesion in Kawasaki Disease and In Vitro Studies Demonstrated That S100A4 Treatment Made HCAECs More Susceptible to Neutrophil Infiltration.

Applications

Unspecified application

Species

Unspecified reactive species

Ken-Pen Weng,Kuang-Jen Chien,Shih-Hui Huang,Lien-Hung Huang,Pei-Hsien Lin,Yuyu Lin,Wei-Hsiang Chang,Chun-Yu Chen,Sung-Chou Li
View all publications
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