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AB316254

Human FOLR3 ELISA Kit

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Human FOLR3 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human FOLR3 with a sensitivity of 1.18 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

Folate receptor gamma, FR-gamma, Folate receptor 3, FOLR3

3 Images
Sandwich ELISA - Human FOLR3 ELISA Kit (AB316254)
  • sELISA

Supplier Data

Sandwich ELISA - Human FOLR3 ELISA Kit (AB316254)

Example of human FOLR3 standard curve. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human FOLR3 ELISA Kit (AB316254)
  • sELISA

Supplier Data

Sandwich ELISA - Human FOLR3 ELISA Kit (AB316254)

Serum of eight individual healthy human female donors was measured in duplicate. Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean FOLR3 concentration of detected samples was determined to be 12,258 pg/mL with a range of undetected – 37,875 pg/mL.

Sandwich ELISA - Human FOLR3 ELISA Kit (AB316254)
  • sELISA

Supplier Data

Sandwich ELISA - Human FOLR3 ELISA Kit (AB316254)

Interpolated concentration of native FOLR3 was measured in duplicate at different sample concentrations. Undiluted samples are as follows : human serum 12.5%, plasma – citrate 6.25%, plasma – EDTA 12.5%, plasma – heparin 6.25%.  The interpolated dilution factor corrected values (to neat) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

Key facts

Detection method

Colorimetric

Sample types

Serum, EDTA Plasma, Citrate plasma, Heparin Plasma

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 1.18 pg/mL

Range

5.469 - 350 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human FOLR3 SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of FOLR3 protein in Serum, EDTA Plasma, Cit Plasma, Hep Plasma. Quantitate Human FOLR3 with 1.18 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
        -High sensitivity, specificity and reproducibility from superior antibodies
        -Fully validated in biological samples
        -96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.4" }, { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "0.1" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.3" }, { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.6" } ]
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Recovery

[ { "sample": "Serum", "range": "91 - 111 %", "average": "= 102" }, { "sample": "EDTA Plasma", "range": "80 - 102 %", "average": "= 90" }, { "sample": "Citrate plasma", "range": "102 - 117 %", "average": "= 108" }, { "sample": "Heparin Plasma", "range": "100 - 115 %", "average": "= 105" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab316254-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB316254-CMP03", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB316254-CMP04", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human FOLR3 Capture Antibody", "number":"AB316254-CMP10", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 5BI", "number":"AB316254-CMP07", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human FOLR3 Lyophilized Recombinant Protein", "number":"AB316254-CMP08", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB316254-CMP06", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB316254-CMP05", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB316254-CMP01", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB316254-CMP02", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human FOLR3 Detector Antibody", "number":"AB316254-CMP09", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

FOLR3 also known as folate receptor gamma is a member of the folate receptor family responsible for binding and transporting folate across cell membranes. This protein has a molecular mass of about 32 kDa. FOLR3 is primarily found in certain tissues including the placenta and liver and it exhibits limited expression in several epithelial tissues. Folate receptors including FOLR3 function by binding folates and delivering them into cells via endocytosis a process essential for the maintenance of regular cellular activity and function.
Biological function summary

Folate receptor gamma plays a significant role in the transport and uptake of folate an important vitamin necessary for DNA synthesis and methylation. The receptor assists in various cellular processes including nucleotide biosynthesis. FOLR3 works as part of a larger complex of folate receptors which ensure cells have adequate folate levels for normal metabolic functions. By doing so it helps facilitate normal cell growth and division critical in rapidly dividing cells.

Pathways

FOLR3 is notably involved in the folate metabolism pathway contributing to DNA synthesis and repair processes. This pathway connects with the one-carbon metabolism pathway where folate is an essential donor of carbons necessary for methylation reactions. In these pathways FOLR3 interacts with other folate receptors like FOLR1 and FOLR2 to maintain efficient folate transport and cellular homeostasis. Ensuring proper folate availability supports homocysteine metabolism which is another critical aspect of these pathways.

FOLR3 relates notably to conditions such as cancer and inflammatory disorders. Cancer cells often exhibit elevated folate receptor expression including FOLR3 due to their increased requirement for folate in rapid cell proliferation. Inflammatory disorders also see changes in folate receptor expression which might affect immune cell functions and inflammation regulation. In these contexts FOLR3 interacts with tumor-associated proteins and immune modulators highlighting its potential as a therapeutic target in medical research and treatment development.
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Product protocols

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Target data

Binds to folate and reduced folic acid derivatives and mediates delivery of 5-methyltetrahydrofolate to the interior of cells. Isoform Short does not bind folate.
See full target information Folate receptor gamma
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websiteProtocolBooklet
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Product promise

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