Human Fractalkine (CX3CL1) ELISA Kit- Extracellular is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human Fractalkine (CX3CL1) - Extracellular in Serum, Citrate plasma, EDTA Plasma, Heparin Plasma, Cell culture supernatant, Urine samples.
Images
Key facts
- Detection method
- Colorimetric
- Sample types
- Serum, Citrate plasma, EDTA Plasma, Heparin Plasma, Cell culture supernatant, Urine
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 54.688 - 3500 pg/mL
- Assay time
- 1h 30m
- Sensitivity
- = 13.41 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Chemokine that acts as a ligand for both CX3CR1 and integrins ITGAV:ITGB3 and ITGA4:ITGB1 (PubMed:12055230, PubMed:21829356, PubMed:23125415, PubMed:9782118, PubMed:9931005). The CX3CR1-CX3CL1 signaling exerts distinct functions in different tissue compartments, such as immune response, inflammation, cell adhesion and chemotaxis (PubMed:12055230, PubMed:9024663, PubMed:9177350, PubMed:9782118). Regulates leukocyte adhesion and migration processes at the endothelium (PubMed:9024663, PubMed:9177350). Can activate integrins in both a CX3CR1-dependent and CX3CR1-independent manner (PubMed:23125415, PubMed:24789099). In the presence of CX3CR1, activates integrins by binding to the classical ligand-binding site (site 1) in integrins (PubMed:23125415, PubMed:24789099). In the absence of CX3CR1, binds to a second site (site 2) in integrins which is distinct from site 1 and enhances the binding of other integrin ligands to site 1 (PubMed:23125415, PubMed:24789099). Processed fractalkine. The soluble form is chemotactic for T-cells and monocytes, but not for neutrophils. Fractalkine. The membrane-bound form promotes adhesion of those leukocytes to endothelial cells. (Microbial infection) Mediates the cytoadherence of erythrocytes infected with parasite P.falciparum (strain 3D7) with endothelial cells by interacting with P.falciparum CBP1 and CBP2 expressed at the surface of erythrocytes (PubMed:27653778). The adhesion prevents the elimination of infected erythrocytes by the spleen (Probable).
Alternative names
FKN, NTT, SCYD1, A-152E5.2, CX3CL1, Fractalkine, C-X3-C motif chemokine 1, CX3C membrane-anchored chemokine, Neurotactin, Small-inducible cytokine D1
What's included?
Human Fractalkine (CX3CL1) ELISA Kit- Extracellular is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human Fractalkine (CX3CL1) - Extracellular in Serum, Citrate plasma, EDTA Plasma, Heparin Plasma, Cell culture supernatant, Urine samples.
Key facts
- Detection method
- Colorimetric
- Sample types
- Serum, Citrate plasma, EDTA Plasma, Heparin Plasma, Cell culture supernatant, Urine
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 54.688 - 3500 pg/mL
- Assay time
- 1h 30m
- Assay Platform
- Microplate (12 x 8 well strips)
- Sensitivity
- = 13.41 pg/mL
- General recovery
- 97%
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Serum | n 8 | mean - | SD - | C.V. = 2.2 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Serum | n 3 | mean - | SD - | C.V. = 1.6 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Cell Lysate | Average % = 103 | Range 101 - 107 % |
Sample type Tissue Extracts | Average % = 109 | Range 106 - 112 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Human Fractalkine (CX3CL1) SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Fractalkine (CX3CL1) protein in Serum, Cit Plasma, EDTA Plasma, Hep Plasma, Cell culture supernatant, Urine, Milk, Cerebral Spinal Fluid. Quantitate Human Fractalkine (CX3CL1) with 13.41 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.
Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips, available in 10-pack (10 x 96-well plates)
-Also available in a fully validated 384-well format.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
8 product images
Sandwich ELISA - Human Fractalkine (CX3CL1) ELISA Kit- Extracellular (ab322378)
Standard curve comparison between Human Fractalkine SimpleStep ELISA kit (ab322378) and traditional ELISA kit from leading competitor. The SimpleStep ELISA kit shows increased sensitivity.
Sandwich ELISA - Human Fractalkine (CX3CL1) ELISA Kit- Extracellular (ab322378)
Interpolated concentration of native Fractalkine was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 12.5% HepG2 supernatant. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.
Sandwich ELISA - Human Fractalkine (CX3CL1) ELISA Kit- Extracellular (ab322378)
Example of human Fractalkine standard curve in Sample Diluent NS in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human Fractalkine (CX3CL1) ELISA Kit- Extracellular (ab322378)
Supernatant from HUVEC cells grown in the presence and absence of 40 ng/ml TNF alpha and 50 ng/mL IFN gamma for 24 hours was measured in duplicate. Interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD n=2). The mean Fractalkine concentration was determined to be 95.58 ng/ml in treated HUVEC supernatant and not detectable in untreated HUVEC supernatant.
Sandwich ELISA - Human Fractalkine (CX3CL1) ELISA Kit- Extracellular (ab322378)
Serum of ten individual healthy human male donors and three individual type 2 diabetes (T2D) donors was measured in duplicate. Interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). The mean Fractalkine concentration in healthy donors was determined to be 710.5 pg/mL with a range of 517.6 – 1,053.6 pg/mL. The mean Fractalkine concentration in type 2 diabetes donors was determined to be 875.4 pg/mL with a range of 828.0 – 926.5 pg/mL.
Sandwich ELISA - Human Fractalkine (CX3CL1) ELISA Kit- Extracellular (ab322378)
Interpolated concentration of native Fractalkine was measured in duplicate at different sample concentrations. Undiluted samples are as follows: HepG2 supernatant 12.5% and milk 25%. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.
Sandwich ELISA - Human Fractalkine (CX3CL1) ELISA Kit- Extracellular (ab322378)
Interpolated concentration of native Fractalkine was measured in duplicate at different sample concentrations. Undiluted samples are serum 50%, plasma (citrate) 50%, plasma (EDTA) 50%, plasma (heparin) 50%, and urine (pooled gender) 25%. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.
Sandwich ELISA - Human Fractalkine (CX3CL1) ELISA Kit- Extracellular (ab322378)
Example of human Fractalkine standard curve in Sample Diluent NS. Background-subtracted data values (mean +/- SD) are graphed.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com