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AB100522

Human Fractalkine ELISA Kit (CX3CL1)

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(2 Publications)

Human Fractalkine ELISA Kit (CX3CL1) is a Sandwich ELISA for the measurement of Human Fractalkine (CX3CL1) in Human in Biofluids, Cell Culture Media samples.

View Alternative Names

FKN, NTT, SCYD1, A-152E5.2, CX3CL1, Fractalkine, C-X3-C motif chemokine 1, CX3C membrane-anchored chemokine, Neurotactin, Small-inducible cytokine D1

2 Images
Sandwich ELISA - Human Fractalkine ELISA Kit (CX3CL1) (AB100522)
  • sELISA

Supplier Data

Sandwich ELISA - Human Fractalkine ELISA Kit (CX3CL1) (AB100522)

Representative Standard Curve using ab100522.

Sandwich ELISA - Human Fractalkine ELISA Kit (CX3CL1) (AB100522)
  • sELISA

Supplier Data

Sandwich ELISA - Human Fractalkine ELISA Kit (CX3CL1) (AB100522)

Representative Standard Curve using ab100522.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

< 300 pg/mL

Range

0.25 - 60 ng/mL

Assay Platform

Microplate

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Primary Antibodies

AB25088

Anti-CX3CL1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CX3CL1 antibody (AB25088)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's CX3CL1 (Fraktaline) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human CX3CL1 in plasma and cell culture supernatants. (Human CX3CL1 concentration is pretty low in normal plasma, it may not be detected in this assay).

This assay employs an antibody specific for human CX3CL1 coated on a 96-well plate. Standards and samples are pipetted into the wells and CX3CL1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human CX3CL1 antibody is added. After washing away unbound biotinylated antibody, HRPconjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CX3CL1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

We have not been able to detect endogenous Human Fractalkine in normal serum with ab100522, only in serum spiked with Human Fractalkine.

Get higher sensitivity in only 90 minutes with Human Fractalkine ELISA Kit (CX3Cl1) (ab192145) from our SimpleStep ELISA® range.

Optimization may be required with urine samples

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Recovery

[ { "sample": "Cell culture supernatant", "range": "83 - 102 %", "average": "= 94.47" }, { "sample": "Plasma", "range": "83 - 103 %", "average": "= 93.54" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab100522-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB100522-CMP01", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB100522-CMP05", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B", "number":"AB100522-CMP04", "productcode":"" }, { "size":"1 x 30 mL", "name":"Assay Diluent A", "number":"AB100522-CMP03", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Recombinant Human CX3CL1 Standard (lyophilized)", "number":"AB100522-CMP08", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated anti-Human CX3CL1", "number":"AB100522-CMP09", "productcode":"" }, { "size":"1 x 200 µL", "name":"320X HRP-Streptavidin Concentrate", "number":"AB100522-CMP02", "productcode":"" }, { "size":"1 x 1 Unit", "name":"CX3CL1 Microplate (12 x 8 well strips)", "number":"AB100522-CMP07", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB100522-CMP06", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CX3CL1 also known as fractalkine is a chemokine protein characterized mechanically by its dual function as both a chemoattractant and adhesion molecule. It exhibits a unique membrane-bound structure and can exist in a soluble form after proteolytic cleavage. The mass of CX3CL1 is approximately 42-45 kDa. This protein is highly expressed in endothelial cells neurons and tissues with substantial vascularization. Considerable interest surrounds the potential of CX3CL1 assessment in various studies often involving applications like fractalkine ELISA or assays on mouse models.
Biological function summary

CX3CL1 is involved in the immune system and nervous system functions influencing leukocyte adhesion and migration. It is not known to be part of a large multiprotein complex but directly interacts with its fractalkine receptor CX3CR1 on the surface of immune cells. This interaction regulates communication between neurons and microglia which highlights its role in neuroinflammation. CX3CL1's expression and function suggest a significant influence over inflammatory responses and homeostatic balance within these systems.

Pathways

CX3CL1 plays a role in the MAPK and PI3K-Akt pathways vital for cell survival proliferation and migration. Within these pathways CX3CL1 influences and interacts with several proteins thereby modulating inflammatory responses and cellular migration. These pathways are essential for immune system regulation where CX3CL1 acts as a mediator in signal transduction processes that connect immune responses to cellular activities such as proliferation and survival.

CX3CL1 has associations with neurodegenerative disorders like Alzheimer's disease and inflammatory diseases such as rheumatoid arthritis. Alterations in CX3CL1 expression or its fractalkine receptor function can influence disease progression by affecting how immune cells communicate and migrate to inflamed or damaged tissues. CX3CR1 the receptor for CX3CL1 represents an important link in these connections driving research efforts to explore therapies targeting these interactions to alleviate disease symptoms.
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Product protocols

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Target data

Chemokine that acts as a ligand for both CX3CR1 and integrins ITGAV : ITGB3 and ITGA4 : ITGB1 (PubMed : 12055230, PubMed : 21829356, PubMed : 23125415, PubMed : 9782118, PubMed : 9931005). The CX3CR1-CX3CL1 signaling exerts distinct functions in different tissue compartments, such as immune response, inflammation, cell adhesion and chemotaxis (PubMed : 12055230, PubMed : 9024663, PubMed : 9177350, PubMed : 9782118). Regulates leukocyte adhesion and migration processes at the endothelium (PubMed : 9024663, PubMed : 9177350). Can activate integrins in both a CX3CR1-dependent and CX3CR1-independent manner (PubMed : 23125415, PubMed : 24789099). In the presence of CX3CR1, activates integrins by binding to the classical ligand-binding site (site 1) in integrins (PubMed : 23125415, PubMed : 24789099). In the absence of CX3CR1, binds to a second site (site 2) in integrins which is distinct from site 1 and enhances the binding of other integrin ligands to site 1 (PubMed : 23125415, PubMed : 24789099).. Processed fractalkine. The soluble form is chemotactic for T-cells and monocytes, but not for neutrophils.. Fractalkine. The membrane-bound form promotes adhesion of those leukocytes to endothelial cells.. (Microbial infection) Mediates the cytoadherence of erythrocytes infected with parasite P.falciparum (strain 3D7) with endothelial cells by interacting with P.falciparum CBP1 and CBP2 expressed at the surface of erythrocytes (PubMed : 27653778). The adhesion prevents the elimination of infected erythrocytes by the spleen (Probable).
See full target information CX3CL1
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Computational and mathematical methods in medicine 2022:1949344 PubMed36118839

2022

CX3CL1 Derived from Bone Marrow Mesenchymal Stem Cells Inhibits A -Induced SH-SY5Y Cell Pathological Damage through TXNIP/NLRP3 Signaling Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Chuan Guo,Qinxuan Li,Jiujia Xiao,Xuhui Zhou,Meichen Tian,Lei Xie,Xun Xia

Journal of immunology research 2020:2932696 PubMed32884948

2020

Comparative Analysis of the Occurrence and Role of CX3CL1 (Fractalkine) and Its Receptor CX3CR1 in Hemophilic Arthropathy and Osteoarthritis.

Applications

Unspecified application

Species

Unspecified reactive species

Piotr Wojdasiewicz,Łukasz A Poniatowski,Andrzej Kotela,Marta Skoda,Michał Pyzlak,Aleksandra Stangret,Ireneusz Kotela,Dariusz Szukiewicz
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