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AB192145

Human Fractalkine ELISA Kit (CX3Cl1)

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(4 Publications)

Human Fractalkine ELISA Kit (CX3Cl1) is a single-wash 90-min Simplestep used to quantify Human Fractalkine (CX3Cl1) with a sensitivity of 1.18 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Validated on a number of sample types including cerebrospinal fluid (CSF)
- Cited in over 5 citations

View Alternative Names

FKN, NTT, SCYD1, A-152E5.2, CX3CL1, Fractalkine, C-X3-C motif chemokine 1, CX3C membrane-anchored chemokine, Neurotactin, Small-inducible cytokine D1

5 Images
Sandwich ELISA - Human Fractalkine ELISA Kit (CX3Cl1) (AB192145)
  • sELISA

Unknown

Sandwich ELISA - Human Fractalkine ELISA Kit (CX3Cl1) (AB192145)

Interpolated concentrations of native Fractalkine in human serum, plasma samples.

The concentrations of Fractalkine were measured in duplicates, interpolated from the Fractalkine standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 25%, plasma (citrate) 12.5%, plasma (heparin) 25% and plasma (EDTA) 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Fractalkine concentration was determined to be 107 pg/mL in serum, 67 pg/mL in plasma (citrate), 86 pg/mL in plasma (heparin) and 100 pg/mL in plasma (EDTA).

Sandwich ELISA - Human Fractalkine ELISA Kit (CX3Cl1) (AB192145)
  • sELISA

Supplier Data

Sandwich ELISA - Human Fractalkine ELISA Kit (CX3Cl1) (AB192145)

Example of Fractalkine standard curve.

Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human Fractalkine ELISA Kit (CX3Cl1) (AB192145)
  • sELISA

Unknown

Sandwich ELISA - Human Fractalkine ELISA Kit (CX3Cl1) (AB192145)

Interpolated concentrations of spike Fractalkine in human cell culture media sample.

The concentrations of Fractalkine were measured in duplicates, interpolated from the Fractalkine standard curves and corrected for sample dilution. Undiluted samples are as follows : cell culture media 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

Sandwich ELISA - Human Fractalkine ELISA Kit (CX3Cl1) (AB192145)
  • sELISA

Unknown

Sandwich ELISA - Human Fractalkine ELISA Kit (CX3Cl1) (AB192145)

Interpolated concentrations of native Fractalkine in human urine, milk and CSF samples.

The concentrations of Fractalkine were measured in duplicates, interpolated from the Fractalkine standard curves and corrected for sample dilution. Undiluted samples are as follows : urine 25%, milk 12.5, and CSF 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Fractalkine concentration was determined to be 71 pg/mL in urine, 127 pg/mL in milk, and 13 pg/mL in CSF.

Sandwich ELISA - Human Fractalkine ELISA Kit (CX3Cl1) (AB192145)
  • sELISA

Supplier Data

Sandwich ELISA - Human Fractalkine ELISA Kit (CX3Cl1) (AB192145)

Interpolated concentrations of Fractalkine in Human serum from 10 donors.

Serum from 10 apparently healthy male donors was measured in duplicate. The mean Fractalkine concentration was determined to be 83 pg/mL with a range of 50-118 pg/mL.

Key facts

Detection method

Colorimetric

Sample types

Cerebral Spinal Fluid, Urine, Heparin Plasma, Citrate plasma, Cell culture supernatant, Milk, Serum, EDTA Plasma

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 1.18 pg/mL

Range

3.91 - 250 pg/mL

Assay time

1h 30m

Assay Platform

Microplate

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Reactivity data

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Product details

Human Fractalkine ELISA Kit (CX3Cl1) (ab192145) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Fractalkine (CX3Cl1) protein in cell culture supernatant, cit plasma, edta plasma, hep plasma, milk, serum, and urine. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Fractalkine (CX3Cl1) with 1.18 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Human milk", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.7" }, { "reproducibilityType": "Intra", "sample": "Human milk", "replicates": 5, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3" } ]
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Recovery

[ { "sample": "Cell culture supernatant", "range": "95 - 98 %", "average": "= 97" }, { "sample": "Serum", "range": "88 - 98 %", "average": "= 94" }, { "sample": "Milk", "range": "75 - 97 %", "average": "= 86" }, { "sample": "EDTA Plasma", "range": "84 - 91 %", "average": "= 88" }, { "sample": "Cerebral Spinal Fluid", "range": "95 - 110 %", "average": "= 102" }, { "sample": "Urine", "range": "92 - 95 %", "average": "= 93" }, { "sample": "Heparin Plasma", "range": "83 - 86 %", "average": "= 84" }, { "sample": "Citrate plasma", "range": "90 - 102 %", "average": "= 97" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CX3CL1 also known as fractalkine is a chemokine protein characterized mechanically by its dual function as both a chemoattractant and adhesion molecule. It exhibits a unique membrane-bound structure and can exist in a soluble form after proteolytic cleavage. The mass of CX3CL1 is approximately 42-45 kDa. This protein is highly expressed in endothelial cells neurons and tissues with substantial vascularization. Considerable interest surrounds the potential of CX3CL1 assessment in various studies often involving applications like fractalkine ELISA or assays on mouse models.
Biological function summary

CX3CL1 is involved in the immune system and nervous system functions influencing leukocyte adhesion and migration. It is not known to be part of a large multiprotein complex but directly interacts with its fractalkine receptor CX3CR1 on the surface of immune cells. This interaction regulates communication between neurons and microglia which highlights its role in neuroinflammation. CX3CL1's expression and function suggest a significant influence over inflammatory responses and homeostatic balance within these systems.

Pathways

CX3CL1 plays a role in the MAPK and PI3K-Akt pathways vital for cell survival proliferation and migration. Within these pathways CX3CL1 influences and interacts with several proteins thereby modulating inflammatory responses and cellular migration. These pathways are essential for immune system regulation where CX3CL1 acts as a mediator in signal transduction processes that connect immune responses to cellular activities such as proliferation and survival.

CX3CL1 has associations with neurodegenerative disorders like Alzheimer's disease and inflammatory diseases such as rheumatoid arthritis. Alterations in CX3CL1 expression or its fractalkine receptor function can influence disease progression by affecting how immune cells communicate and migrate to inflamed or damaged tissues. CX3CR1 the receptor for CX3CL1 represents an important link in these connections driving research efforts to explore therapies targeting these interactions to alleviate disease symptoms.
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Product protocols

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Target data

Chemokine that acts as a ligand for both CX3CR1 and integrins ITGAV : ITGB3 and ITGA4 : ITGB1 (PubMed : 12055230, PubMed : 21829356, PubMed : 23125415, PubMed : 9782118, PubMed : 9931005). The CX3CR1-CX3CL1 signaling exerts distinct functions in different tissue compartments, such as immune response, inflammation, cell adhesion and chemotaxis (PubMed : 12055230, PubMed : 9024663, PubMed : 9177350, PubMed : 9782118). Regulates leukocyte adhesion and migration processes at the endothelium (PubMed : 9024663, PubMed : 9177350). Can activate integrins in both a CX3CR1-dependent and CX3CR1-independent manner (PubMed : 23125415, PubMed : 24789099). In the presence of CX3CR1, activates integrins by binding to the classical ligand-binding site (site 1) in integrins (PubMed : 23125415, PubMed : 24789099). In the absence of CX3CR1, binds to a second site (site 2) in integrins which is distinct from site 1 and enhances the binding of other integrin ligands to site 1 (PubMed : 23125415, PubMed : 24789099).. Processed fractalkine. The soluble form is chemotactic for T-cells and monocytes, but not for neutrophils.. Fractalkine. The membrane-bound form promotes adhesion of those leukocytes to endothelial cells.. (Microbial infection) Mediates the cytoadherence of erythrocytes infected with parasite P.falciparum (strain 3D7) with endothelial cells by interacting with P.falciparum CBP1 and CBP2 expressed at the surface of erythrocytes (PubMed : 27653778). The adhesion prevents the elimination of infected erythrocytes by the spleen (Probable).
See full target information CX3CL1
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Experimental and therapeutic medicine 26:359 PubMed37324510

2023

CX3CL1/CX3CR1 axis alleviates inflammation and apoptosis in human nucleus pulpous cells via M2 macrophage polarization.

Applications

Unspecified application

Species

Unspecified reactive species

Xiao-Wen Gao,Han-Lin Hu,Ming-Hua Xie,Cai-Xia Tang,Jun Ou,Zheng-Hao Lu

EMBO reports 23:e55871 PubMed36200589

2022

Endothelial-immune crosstalk contributes to vasculopathy in nonalcoholic fatty liver disease.

Applications

Unspecified application

Species

Unspecified reactive species

Chun-Yi Ng,Khang Leng Lee,Mark Dhinesh Muthiah,Kan Xing Wu,Florence Wen Jing Chioh,Konstanze Tan,Gwyneth Shook Ting Soon,Asim Shabbir,Wai Mun Loo,Zun Siong Low,Qingfeng Chen,Walter Wahli,Nguan Soon Tan,Huck Hui Ng,Yock Young Dan,Christine Cheung

EMBO reports 23:e54271 PubMed35403791

2022

Endothelial-immune crosstalk contributes to vasculopathy in nonalcoholic fatty liver disease.

Applications

Unspecified application

Species

Unspecified reactive species

Chun-Yi Ng,Khang Leng Lee,Mark Dhinesh Muthiah,Kan Xing Wu,Florence Wen Jing Chioh,Konstanze Tan,Gwyneth Shook Ting Soon,Asim Shabbir,Wai Mun Loo,Zun Siong Low,Qingfeng Chen,Nguan Soon Tan,Huck Hui Ng,Yock Young Dan,Christine Cheung

Frontiers in immunology 11:601639 PubMed33552057

2021

Evaluation of Proteoforms of the Transmembrane Chemokines CXCL16 and CX3CL1, Their Receptors, and Their Processing Metalloproteinases ADAM10 and ADAM17 in Proliferative Diabetic Retinopathy.

Applications

Unspecified application

Species

Unspecified reactive species

Ahmed M Abu El-Asrar,Mohd Imtiaz Nawaz,Ajmal Ahmad,Alexandra De Zutter,Mohammad Mairaj Siddiquei,Marfa Blanter,Eef Allegaert,Priscilla W Gikandi,Gert De Hertogh,Jo Van Damme,Ghislain Opdenakker,Sofie Struyf
View all publications
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Product promise

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For full details, please see our Terms & Conditions
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Associated Products

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