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Human Frataxin ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human Frataxin in Cell culture extracts, Tissue Extracts samples.

Detection method

Colorimetric

Sample types

Cell culture extracts, Tissue Extracts

Assay type

Sandwich (quantitative)

Reactive species

Human

Range

9.88 - 800 pg/mL

Assay time

1h 30m

Sensitivity

= 2.1 pg/mL

Reactivity data

Application
sELISA
Reactivity
Reacts
Dilution info
-
Notes

-

Images

Target data

What's included?

1 x 384 Tests
Components
10X Human Frataxin Capture Antibody
1 x 600 µL
10X Human Frataxin Detector Antibody
1 x 600 µL
10X Wash Buffer PT (ab206977)
1 x 20 mL
384 well CaptSure™ microplates
1 x 1 Unit
50X Cell Extraction Enhancer Solution (ab193971)
6 x 1 mL
5X Cell Extraction Buffer PTR (ab193970)
1 x 50 mL
Antibody Diluent 4BI
1 x 6 mL
Human Frataxin Lyophilized Recombinant Protein
2 x 1 Vial
Plate Seals
1 x 1 Unit
Stop Solution
2 x 12 mL
TMB Development Solution
2 x 12 mL

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Human Frataxin ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human Frataxin in Cell culture extracts, Tissue Extracts samples.

Key facts

Detection method

Colorimetric

Sample types

Cell culture extracts, Tissue Extracts

Assay type

Sandwich (quantitative)

Reactive species

Human

Range

9.88 - 800 pg/mL

Assay time

1h 30m

Assay Platform

Microplate

Sensitivity

= 2.1 pg/mL

Precision

Intra assay

Sample
HeLa
n
9
mean
-
SD
-
C.V.
2.3

Inter assay

Sample
HeLa
n
3
mean
-
SD
-
C.V.
6.3

Recovery

Sample specific recovery

Sample type
Cell culture media
Average %
= 97
Range
93 - 101 %
Sample type
EDTA Plasma
Average %
= 89
Range
88 - 92 %
Sample type
Serum
Average %
= 92
Range
86 - 98 %

Storage

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

Notes

Human Frataxin ELISA kit (ab176112) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Frataxin protein in human cell culture and tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate human Frataxin with 1.1 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpeStep ELISA® kits.

ASSAY SPECIFICITY This kit recognizes both native and recombinant human Frataxin protein in cell and tissue extract samples only. Serum, plasma, saliva, and urine samples have not been tested with this kit.

Frataxin is a 17 kDa nuclear-encoded mitochondrial protein. In humans the gene is localized on chromosome 9 and is highly conserved during evolution. The gene is expressed in every cell, although in varying levels in different tissues and during development. The specific function of frataxin is still unknown, but it has been shown to play a role in iron metabolism. Studies have demonstrated that the deletion of the frataxin gene in yeast results in iron accumulation in mitochondria and loss of respiration. Recombinant human frataxin has been shown to bind iron in vitro, and increased mitochondrial iron levels have been observed in patients with Friedreich's Ataxia (FRDA). FRDA is an autosomal recessive, progressive degenerative disease characterized by neurodegeneration and cardiomyopathy it is the most common inherited ataxia.

Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

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