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AB322428

Human FRS2 ELISA Kit

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Human FRS2 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human FRS2 with a sensitivity of 11.95 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 10x96 plates for bulk orders

View Alternative Names

Fibroblast growth factor receptor substrate 2, FGFR substrate 2, FGFR-signaling adaptor SNT, Suc1-associated neurotrophic factor target 1, SNT-1, FRS2

2 Images
Sandwich ELISA - Human FRS2 ELISA Kit (AB322428)
  • sELISA

Supplier Data

Sandwich ELISA - Human FRS2 ELISA Kit (AB322428)

Interpolated concentration of native FRS2 was measured in duplicate at different sample concentrations. Undiluted samples are 500 µg/mL MCF7 cell extract, 200 µg/mL NIH/3T3 cell extract, and 250 µg/mL PC-12 cell extract. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer SSW.

Sandwich ELISA - Human FRS2 ELISA Kit (AB322428)
  • sELISA

Supplier Data

Sandwich ELISA - Human FRS2 ELISA Kit (AB322428)

Example of human FRS2 standard curve. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Cell Lysate, Tissue Extracts

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 11.95 pg/mL

Range

37.5 - 2400 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human FRS2 SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of FRS2 protein in Cell Lysate and Tissue extracts. Quantitate Human FRS2 with 11.95 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips, available in 10-pack (10 x 96-well plates)

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Extract", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.7" }, { "reproducibilityType": "Inter", "sample": "Extract", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.5" } ]
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Recovery

[ { "sample": "Cell Lysate", "range": "96 - 103 %", "average": "= 100" }, { "sample": "Tissue Extracts", "range": "94 - 97 %", "average": "= 95" } ]
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What's included?

{ "values": { "10x96Tests": { "sellingSize": "10 x 96 Tests", "publicAssetCode":"ab322428-10x96Tests", "assetComponentDetails": [ { "size":"2 x 50 mL", "name":"Cell Extraction Buffer SSW", "number":"AB322428-CMP07" }, { "size":"1 x 120 mL", "name":"Stop Solution", "number":"AB322428-CMP04" }, { "size":"1 x 120 mL", "name":"TMB Development Solution", "number":"AB322428-CMP03" }, { "size":"10 x 600 µL", "name":"10X Human FRS2 Detector Antibody", "number":"AB322428-CMP08" }, { "size":"10 x 600 µL", "name":"10X Human FRS2 Capture Antibody", "number":"AB322428-CMP01" }, { "size":"20 x 1 Vial", "name":"Human FRS2 Lyophilized Recombinant Protein", "number":"AB322428-CMP09" }, { "size":"1 x 200 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB322428-CMP11" }, { "size":"10 x 6 mL", "name":"Antibody Diluent 4BR", "number":"AB322428-CMP10" }, { "size":"10 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB322428-CMP05" }, { "size":"10 x 1 Unit", "name":"Plate Seal", "number":"AB322428-CMP06" } ] }, "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab322428-1x96Tests", "assetComponentDetails": [ { "size":"1 x 10 mL", "name":"Cell Extraction Buffer SSW", "number":"AB322428-CMP07", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB322428-CMP04", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB322428-CMP03", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human FRS2 Detector Antibody", "number":"AB322428-CMP08", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human FRS2 Capture Antibody", "number":"AB322428-CMP01", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human FRS2 Lyophilized Recombinant Protein", "number":"AB322428-CMP09", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB322428-CMP02", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BR", "number":"AB322428-CMP10", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB322428-CMP11", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB322428-CMP05", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seal", "number":"AB322428-CMP06", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

Adapter protein that links activated FGR and NGF receptors to downstream signaling pathways. Plays an important role in the activation of MAP kinases and in the phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, in response to ligand-mediated activation of FGFR1. Modulates signaling via SHC1 by competing for a common binding site on NTRK1.
See full target information FRS2
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websiteProtocolBooklet
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com