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AB267653

Human FRZB ELISA Kit (sFRP-3)

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Human FRZB ELISA Kit (sFRP-3) is a Sandwich (quantitative) ELISA for the measurement of Human FRZB (sFRP-3) in Human in Biofluids, Cell Culture Media samples.

View Alternative Names

FIZ, FRE, FRP, FRZB1, SFRP3, FRZB, Secreted frizzled-related protein 3, sFRP-3, Frezzled, Fritz, Frizzled-related protein 1, FrzB-1

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Sandwich ELISA - Human FRZB ELISA Kit (sFRP-3) (AB267653)
  • sELISA

Supplier Data

Sandwich ELISA - Human FRZB ELISA Kit (sFRP-3) (AB267653)

This standard curve is for demonstration only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 190 pg/mL

Range

190 - 12000 pg/mL

Assay Platform

Microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human FRZB ELISA Kit (sFRP-3) is designed for the quantitative determination of FRZB in cell culture supernatants, plasma and serum samples.

This assay employs an antibody specific for human FRZB coated on a 96-well plate. Standards and samples are pipetted into the wells and FRZB present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human FRZB antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of FRZB bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

This ELISA kit shows no cross-reactivity with the following cytokines tested: Human 2B4, ADAM-9, APRIL, BMP-2, BMP-9, DcR3, FABP-2, FAP, FGF-19, Galectin-3, HGFR, IFN-alpha/betaR2, IGF-2R, IL-1R6, IL-24, IL-33, Kallikrein 14, Legumain, LOX-1, MBL, Neprilysin, Notch-1, NOV, Osteoactivin, PD-1, PGRPs, Serpin A4, Thrombomodulin, TLP2, Transferrin, WIF-1.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 12" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" } ]
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Recovery

[ { "sample": "Serum", "range": "91 - 108 %", "average": "= 100.1" }, { "sample": "Plasma", "range": "71 - 89 %", "average": "= 76.99" }, { "sample": "Cell culture media", "range": "78 - 102 %", "average": "= 91.66" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab267653-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB267653-CMP06", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB267653-CMP03", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Anti-Human FRZB coated Microplate (12 x 8 wells)", "number":"AB267653-CMP08", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human FRZB Standard (Lyophilized)", "number":"AB267653-CMP01", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Anti-Human FRZB Detection Antibody", "number":"AB267653-CMP07", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Substrate Solution", "number":"AB267653-CMP04", "productcode":"" }, { "size":"1 x 200 µL", "name":"200X HRP-Streptavidin Concentrate", "number":"AB267653-CMP05", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent", "number":"AB267653-CMP02", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

FRZB also known as secreted frizzled-related protein 3 (SFRP3) functions as a modulator of Wnt signaling pathways. This protein has a mass of approximately 36 kDa and serves as an antagonist to the Wnt proteins by binding to them preventing their interaction with cell surface receptors. FRZB is expressed in several tissues with notable levels in cartilage bone and the heart.
Biological function summary

The modulation of Wnt signaling by FRZB plays a significant role in embryonic development and tissue homeostasis. FRZB is not part of a protein complex but has important interactions with other components of the signaling cascade. It influences cell differentiation proliferation and apoptosis which are critical processes during development and tissue repair.

Pathways

FRZB influences both Wnt/β-catenin and non-canonical Wnt pathways. Its interaction with these pathways regulates signal transmission that affects cellular behaviors. Related proteins include Wnt ligands and frizzled receptors which are central to these signaling mechanisms.

Abnormalities in FRZB expression or function show a connection to osteoarthritis and certain cancers. In osteoarthritis FRZB interacts with Wnt proteins which contribute to the degradation of cartilage. In cancer dysregulation of Wnt signaling involving FRZB can lead to unchecked cell proliferation. Understanding its role in these diseases can aid in developing therapeutic strategies.
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Product protocols

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Target data

Soluble frizzled-related proteins (sFRPS) function as modulators of Wnt signaling through direct interaction with Wnts. They have a role in regulating cell growth and differentiation in specific cell types. SFRP3/FRZB appears to be involved in limb skeletogenesis. Antagonist of Wnt8 signaling. Regulates chondrocyte maturation and long bone development.
See full target information FRZB
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