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AB213782

Human FSTL1 ELISA Kit

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(1 Publication)

Human FSTL1 ELISA Kit is a Sandwich ELISA for the measurement of Human FSTL1 in Human in Cell/Tissue Extracts, Cell Culture Media, Biofluids samples.

View Alternative Names

FRP, FSTL1, Follistatin-related protein 1, Follistatin-like protein 1

1 Images
Sandwich ELISA - Human FSTL1 ELISA Kit (AB213782)
  • sELISA

Supplier Data

Sandwich ELISA - Human FSTL1 ELISA Kit (AB213782)

Human FSTL1 ELISA Kit (ab213782) Standard Curve.

Key facts

Detection method

Colorimetric

Sample types

Cell Lysate, Cell culture supernatant, EDTA Plasma, Heparin Plasma, Serum, Tissue Homogenate

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

< 10 pg/mL

Range

312 - 20000 pg/mL

Assay time

3h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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AB174443

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

The Human FSTL1 Enzyme-Linked Immunosorbent Assay (ELISA) kit (ab213782) is designed for the quantitative measurement of Human FSTL1 in cell culture supernatants, cell lysates, tissue homogenates, serum and plasma (heparin, EDTA).

The ELISA kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for FSTL1 has been pre-coated onto 96-well plates. Standards (Expression system for standard: NSO; Immunogen sequence: M1-I308) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for FSTL1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB is used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human FSTL1 amount of sample captured in plate.

Follistatin-related protein 1, also called FSTL1 or FRP is a protein that in humans is encoded by the FSTL1 gene. FSTL1 gene is mapped to chromosome 3q13.33 based on an alignment of FSTL1 sequence with the genomic sequence (GRCh37). This gene encodes a protein with similarity to follistatin, an activin-binding protein. It contains an FS module, a follistatin-like sequence containing 10 conserved cysteine residues. This gene product is thought to be an autoantigen associated with rheumatoid arthritis. This gene may modulate the action of some growth factors on cell proliferation and differentiation. It was suggested that fibroblast-secreted FSTL1 acts on macrophages to increase TNF and IL1B production and IL6 activity.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Sample 1", "replicates": 24, "mean": "1.25 ng/mL", "standardDeviation": "0.094", "coefficientOfVariability": "7.5" }, { "reproducibilityType": "Inter", "sample": "Sample 2", "replicates": 24, "mean": "7.5 ng/mL", "standardDeviation": "0.48", "coefficientOfVariability": "6.4" }, { "reproducibilityType": "Inter", "sample": "Sample 3", "replicates": 24, "mean": "14.4 ng/mL", "standardDeviation": "1.123", "coefficientOfVariability": "7.8" }, { "reproducibilityType": "Intra", "sample": "Sample 1", "replicates": 16, "mean": "1.34 ng/mL", "standardDeviation": "0.056", "coefficientOfVariability": "4.2" }, { "reproducibilityType": "Intra", "sample": "Sample 2", "replicates": 16, "mean": "7.2 ng/mL", "standardDeviation": "0.382", "coefficientOfVariability": "5.3" }, { "reproducibilityType": "Intra", "sample": "Sample 3", "replicates": 16, "mean": "13.9 ng/mL", "standardDeviation": "0.89", "coefficientOfVariability": "6.4" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

FSTL1 also known as FRP is a protein that plays an important role in various cellular functions. The protein is a glycoprotein with a molecular mass of approximately 32 kDa and is expressed in a variety of tissues including heart lung and placenta. FSTL1 contains several domains that facilitate its interaction with other proteins and cellular components. It is located both intracellularly and secreted in the extracellular matrix contributing to its role in cell signaling and structural integrity.
Biological function summary

FSTL1 serves as a modulator in inflammatory and developmental processes. It is a part of a complex that regulates immune responses and cell growth. The protein has been shown to affect the activity of multiple growth factors including Bone Morphogenetic Proteins (BMPs) revealing its regulatory capacity in tissue development and repair. FSTL1 can influence cell differentiation proliferation and survival highlighting its significance in maintaining cellular homeostasis.

Pathways

FSTL1 has important roles in the regulation of the BMP signaling and Wnt pathways. In the BMP pathway FSTL1 interacts with other signaling molecules to modulate pathway intensity and cellular outcomes. The protein links with BMP proteins to influence processes like bone formation and tissue repair. In the Wnt pathway FSTL1 can modulate key interactions that affect cellular proliferation and development. Through these pathways FSTL1 also relates to proteins such as TGF-beta which participate in various cellular processes.

FSTL1 has relevance in conditions such as rheumatoid arthritis and pulmonary hypertension. The protein shows increased expression in inflammatory environments indicating its involvement in rheumatoid arthritis where it engages with immune response pathways. The connection with pulmonary hypertension also positions FSTL1 as a significant factor in cardiovascular pathologies. In both conditions FSTL1 interacts with cytokines and other immune-related proteins influencing disease progression and response to treatment.
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Product protocols

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Target data

Secreted glycoprotein that is involved in various physiological processes, such as angiogenesis, regulation of the immune response, cell proliferation and differentiation (PubMed : 22265692, PubMed : 29212066). Plays a role in the development of the central nervous system, skeletal system, lungs, and ureter (By similarity). Promotes endothelial cell survival, migration and differentiation into network structures in an AKT-dependent manner. Also promotes survival of cardiac myocytes (By similarity). Initiates various signaling cascades by activating different receptors on the cell surface such as DIP2A, TLR4 or BMP receptors (PubMed : 20054002, PubMed : 22265692).
See full target information FSTL1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in cardiovascular medicine 9:1050310 PubMed36684598

2023

Role of follistatin-like 1 levels and functions in calcific aortic stenosis.

Applications

Unspecified application

Species

Unspecified reactive species

Qianru Zhang,Jiawen Ye,Gan Yang,Ling Yang,Zhongli Chen,Ke Yang,Jia Teng Sun,Yan Liu
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Product promise

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