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AB302766

Human FSTL1/FRP ELISA Kit

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Human FSTL1/FRP ELISA Kit is a single-wash 90-min Simplestep used to quantify Human FSTL1/FRP with a sensitivity of 11.761 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader

View Alternative Names

FRP, FSTL1, Follistatin-related protein 1, Follistatin-like protein 1

3 Images
Sandwich ELISA - Human FSTL1/FRP ELISA Kit (AB302766)
  • sELISA

Supplier Data

Sandwich ELISA - Human FSTL1/FRP ELISA Kit (AB302766)

Serum of ten individual healthy human male donors was measured in duplicate. Interpolated dilution factor corrected values are plotted (mean +- SD, n=2). The mean FSTL1/FRP concentration was determined to be 35.10 ng/mL with a range of 24.29 – 48.74 ng/mL

Sandwich ELISA - Human FSTL1/FRP ELISA Kit (AB302766)
  • sELISA

Supplier Data

Sandwich ELISA - Human FSTL1/FRP ELISA Kit (AB302766)

Example of human FSTL1/FRP standard curve. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human FSTL1/FRP ELISA Kit (AB302766)
  • sELISA

Supplier Data

Sandwich ELISA - Human FSTL1/FRP ELISA Kit (AB302766)

Interpolated concentration of native FSTL1/FRP was measured in duplicate at different sample concentrations. Undiluted samples are 15% serum, plasma (heparin), plasma (EDTA) and, plasma (citrate). The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

Key facts

Detection method

Colorimetric

Sample types

Serum, EDTA Plasma, Citrate plasma, Heparin Plasma, Cell culture media

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 11.761 pg/mL

Range

62.5 - 4000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human FSTL1/FRP SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human FSTL1/FRP protein in human serum, plasma (heparin), plasma (EDTA), plasma (citrate), and cell culture supernatant. Quantitate Human FSTL1/FRP with 11.761 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 4, "mean": null, "standardDeviation": null, "coefficientOfVariability": "12.8" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "13" } ]
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Recovery

[ { "sample": "Cell culture media", "range": "110 - 115 %", "average": "= 113" }, { "sample": "Citrate plasma", "range": "102 - 112 %", "average": "= 107" }, { "sample": "EDTA Plasma", "range": "99 - 110 %", "average": "= 104" }, { "sample": "Heparin Plasma", "range": "101 - 104 %", "average": "= 102" }, { "sample": "Serum", "range": "90 - 104 %", "average": "= 96" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

FSTL1 also known as FRP is a protein that plays an important role in various cellular functions. The protein is a glycoprotein with a molecular mass of approximately 32 kDa and is expressed in a variety of tissues including heart lung and placenta. FSTL1 contains several domains that facilitate its interaction with other proteins and cellular components. It is located both intracellularly and secreted in the extracellular matrix contributing to its role in cell signaling and structural integrity.
Biological function summary

FSTL1 serves as a modulator in inflammatory and developmental processes. It is a part of a complex that regulates immune responses and cell growth. The protein has been shown to affect the activity of multiple growth factors including Bone Morphogenetic Proteins (BMPs) revealing its regulatory capacity in tissue development and repair. FSTL1 can influence cell differentiation proliferation and survival highlighting its significance in maintaining cellular homeostasis.

Pathways

FSTL1 has important roles in the regulation of the BMP signaling and Wnt pathways. In the BMP pathway FSTL1 interacts with other signaling molecules to modulate pathway intensity and cellular outcomes. The protein links with BMP proteins to influence processes like bone formation and tissue repair. In the Wnt pathway FSTL1 can modulate key interactions that affect cellular proliferation and development. Through these pathways FSTL1 also relates to proteins such as TGF-beta which participate in various cellular processes.

FSTL1 has relevance in conditions such as rheumatoid arthritis and pulmonary hypertension. The protein shows increased expression in inflammatory environments indicating its involvement in rheumatoid arthritis where it engages with immune response pathways. The connection with pulmonary hypertension also positions FSTL1 as a significant factor in cardiovascular pathologies. In both conditions FSTL1 interacts with cytokines and other immune-related proteins influencing disease progression and response to treatment.
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Product protocols

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Target data

Secreted glycoprotein that is involved in various physiological processes, such as angiogenesis, regulation of the immune response, cell proliferation and differentiation (PubMed : 22265692, PubMed : 29212066). Plays a role in the development of the central nervous system, skeletal system, lungs, and ureter (By similarity). Promotes endothelial cell survival, migration and differentiation into network structures in an AKT-dependent manner. Also promotes survival of cardiac myocytes (By similarity). Initiates various signaling cascades by activating different receptors on the cell surface such as DIP2A, TLR4 or BMP receptors (PubMed : 20054002, PubMed : 22265692).
See full target information FSTL1
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websiteProtocolBooklet
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