Human Furin ELISA Kit
- Recombinant
- SimpleStep
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Human Furin ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Furin with a sensitivity of 0.05 ng/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
View Alternative Names
FUR, PACE, PCSK3, FURIN, Furin, Dibasic-processing enzyme, Paired basic amino acid residue-cleaving enzyme
- sELISA
Supplier Data
Sandwich ELISA - Human Furin ELISA Kit (AB282292)
Serum from eight individual healthy human female donors was measured in duplicate.
Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Furin concentration was determined to be 3.8 ng/mL with a range of 2.2 – 8.3 ng/mL.
- sELISA
Supplier Data
Sandwich ELISA - Human Furin ELISA Kit (AB282292)
Interpolated concentrations of native Furin in human plasma (EDTA), plasma (Citrate), plasma (Heparin), serum, and saliva.
The concentrations of Furin were measured in duplicates, interpolated from the target standard curves and corrected for sample dilution. Undiluted samples are as follows : 50% plasma (EDTA), 50% plasma (Citrate), 50% Plasma (Heparin), 25% serum, and 7.5% saliva. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean target concentration was determined to be 4.8 ng/mL in plasma (EDTA), 4.1 ng/mL in plasma (Citrate) and 4 ng/mL in and plasma (Heparin), 4.6 ng/mL in serum, and 76 ng/mL in saliva.
- sELISA
Supplier Data
Sandwich ELISA - Human Furin ELISA Kit (AB282292)
PBMC cells were stimulated with 1.5% PHA-M or vehicle control in RPMI containing 10 % FBS and incubated for 46 hours.
The concentrations of Furin were measured in duplicate and interpolated from the Furin standard curves. Undiluted samples are PHA-M stimulated PBMC cell culture supernatant 50% and unstimulated PBMC cell culture supernatant 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Furin concentration was calculated to be 2.24 ng/mL in PHA-M stimulated PBMC cell culture supernatant and undetectable in the unstimulated PBMC control.
- sELISA
Supplier Data
Sandwich ELISA - Human Furin ELISA Kit (AB282292)
Example of human Furin standard curve in Sample Diluent NS.
The Furin standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
Reactivity data
Product details
Human Furin ELISA kit (ab282292) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human Furin protein in human plasma, serum, saliva and cell culture supernatant samples. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Furin with 0.05 ng/mL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Furin, also referred to as dibasic-processing enzyme and Paired basic amino acid residue-cleaving enzyme (PACE), is a calcium-dependent serine endoprotease enzyme encoded by the FURIN gene. Furin belongs to the subtilisin-like proprotein convertase family S8. An 81 amino acid long inhibition peptide acts as a chaperone, is autocatalytically removed in the endoplasmic reticulum, and remains non-covalently bound until a second cleavage occurs in the trans Golgi. Furin acts on many substrates including but not limited to proparathroid hormone, transforming growth factor beta 1, proalbumin, and von Willebrand factor. Furin also acts on virus and is thought to activate the HIV envelope glycoproteins gp160 and gp140, and is thought to cleave the S1/S2 site of the spike protein of the SARS-CoV-2 coronavirus.
Precision
Recovery
What's included?
Properties and storage information
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
Furin processes precursor proteins by cleaving at specific sequence motifs. This activity is essential for the maturation of many hormones growth factors and receptors. Furin is not part of a larger complex; it functions independently in its proteolytic activity. The processing by furin ensures that proteins become functional affecting numerous biological processes across different cell types and tissues.
Pathways
The activity of furin impacts the regulation of the Notch signaling pathway and the MAPK/ERK pathway. In the Notch signaling pathway furin processes Notch receptors facilitating their activation and downstream signaling. In the MAPK/ERK pathway furin activates several key proteins including growth factors and receptors essential for cell proliferation and differentiation. Furin-related proteins such as convertase enzymes are part of a similar family having roles in overlapping or complementary pathways.
Product protocols
- Visit the General protocols
- Visit the Troubleshooting
- Download websiteProtocolBooklet|en
Target data
Complementary Products
Primary Antibodies
AB281204
Anti-Furin antibody [EPR23363-111] - BSA and Azide free (Capture)
RabMAb|Recombinant
![Sandwich ELISA - Anti-Furin antibody [EPR23363-111] - BSA and Azide free (Capture) (AB281204)](https://content.abcam.com/products/images/furin-antibody-epr23363-111-bsa-and-azide-free-capture-ab281204--sandwich-elisa-img165426.jpg)
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Product promise
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